ID AP1_ARATH Reviewed; 256 AA. AC P35631; Q8LA98; Q9LQA8; DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot. DT 20-JUN-2002, sequence version 2. DT 03-NOV-2009, entry version 88. DE RecName: Full=Floral homeotic protein APETALA 1; DE AltName: Full=Agamous-like MADS-box protein AGL7; GN Name=AP1; Synonyms=AGL7; OrderedLocusNames=At1g69120; ORFNames=F4N2.9; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Landsberg erecta; RX MEDLINE=93063372; PubMed=1359429; DOI=10.1038/360273a0; RA Mandel M.A., Gustafson-Brown C., Savidge B., Yanofsky M.F.; RT "Molecular characterization of the Arabidopsis floral homeotic gene RT APETALA1."; RL Nature 360:273-277(1992). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., RA White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., RA Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., RA Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., RA Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., RA Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., RA Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., RA Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., RA Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., RA Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., RA Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., RA Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., RA Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., RA Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., RA Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis RT thaliana."; RL Nature 408:816-820(2000). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE OF 1-251, AND VARIANTS. RC STRAIN=cv. Bla-1, cv. Bretagny, cv. Bs-1, cv. Bu-0, cv. Bu-2, RC cv. Chi-1, cv. Co-1, cv. Columbia, cv. Cvi-0, cv. Gr-3, cv. Ita-0, RC cv. Jl-1, cv. Kas-1, cv. Kent, cv. Landsberg erecta, cv. Li-3, RC cv. Li-8, cv. Lisse, and cv. Wassilewskija; RX MEDLINE=21969421; PubMed=11973317; RA Olsen K.M., Womack A., Garrett A.R., Suddith J.I., Purugganan M.D.; RT "Contrasting evolutionary forces in the Arabidopsis thaliana floral RT developmental pathway."; RL Genetics 160:1641-1650(2002). RN [6] RP FUNCTION. RA Bowman J.L., Alvarez J., Weigel D., Meyerowitz E.M., Smyth D.R.; RT "Control of flower development in Arabidopsis thaliana by APETALA1 and RT interacting genes."; RL Development 119:721-743(1993). RN [7] RP FUNCTION. RA Weigel D., Meyerowitz E.M.; RT "Activation of floral homeotic genes in Arabidopsis."; RL Science 261:1723-1726(1993). RN [8] RP CHARACTERIZATION. RX MEDLINE=96209811; PubMed=8643482; DOI=10.1073/pnas.93.10.4793; RA Riechmann J.L., Krizek B.A., Meyerowitz E.M.; RT "Dimerization specificity of Arabidopsis MADS domain homeotic proteins RT APETALA1, APETALA3, PISTILLATA, and AGAMOUS."; RL Proc. Natl. Acad. Sci. U.S.A. 93:4793-4798(1996). RN [9] RP GENETIC REGULATION. RX PubMed=9783581; DOI=10.1038/26903; RA Parcy F., Nilsson O., Busch M.A., Lee I., Weigel D.; RT "A genetic framework for floral patterning."; RL Nature 395:561-566(1998). RN [10] RP FUNCTION. RX MEDLINE=21178805; PubMed=11283333; DOI=10.1105/tpc.13.4.739; RA Ng M., Yanofsky M.F.; RT "Activation of the Arabidopsis B class homeotic genes by APETALA1."; RL Plant Cell 13:739-753(2001). RN [11] RP CHARACTERIZATION. RX MEDLINE=21074934; PubMed=11206550; DOI=10.1038/35054083; RA Honma T., Goto K.; RT "Complexes of MADS-box proteins are sufficient to convert leaves into RT floral organs."; RL Nature 409:525-529(2001). CC -!- FUNCTION: Probable transcription factor that promotes early floral CC meristem identity in synergy with LEAFY. Is required subsequently CC for the transition of an inflorescence meristem into a floral CC meristem. Is indispensable for normal development of sepals and CC petals in the wild-type flower. Regulates positively the B class CC homeotic proteins APETALA3 and PISTILLATA with the cooperation of CC LEAFY and UFO. Interacts with SEPALLATA3 or AP3/PI heterodimer to CC form complexes that could be involved in genes regulation during CC floral meristem development. Regulates positively AGAMOUS in CC cooperation with LEAFY. Displays a redundant function with CC CAULIFLOWER in the up-regulation of LEAFY. CC -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences. CC Heterodimer with SEP3. Binds AP3/PI to form a ternary complex. CC -!- INTERACTION: CC O82794:AGL24; NbExp=3; IntAct=EBI-592003, EBI-592083; CC O22456:SEP3; NbExp=2; IntAct=EBI-592003, EBI-592020; CC O22456-2:SEP3; NbExp=2; IntAct=EBI-592003, EBI-592035; CC Q8W234:SEU; NbExp=1; IntAct=EBI-592003, EBI-1771131; CC O64645:SOC1; NbExp=3; IntAct=EBI-592003, EBI-592041; CC Q9FVC1:SVP; NbExp=2; IntAct=EBI-592003, EBI-592058; CC Q8RYD9:TT16; NbExp=2; IntAct=EBI-592003, EBI-621993; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- TISSUE SPECIFICITY: Expressed in young flower primordia, later CC becomes localized to sepals and petals. CC -!- DEVELOPMENTAL STAGE: Expressed at an early stage of floral CC initiation. CC -!- INDUCTION: Negatively regulated by TFL1 and by the C class floral CC homeotic protein AGAMOUS. Positively regulated by CAULIFLOWER. CC -!- MISCELLANEOUS: Mutations in the AP1 gene result in the partial CC conversion of flowers into shoots and a disruption of sepal and CC petal development. CC -!- SIMILARITY: Contains 1 K-box domain. CC -!- SIMILARITY: Contains 1 MADS-box domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z16421; CAA78909.1; -; mRNA. DR EMBL; AC008262; AAF27070.1; -; Genomic_DNA. DR EMBL; BT004113; AAO42136.1; -; mRNA. DR EMBL; BT004951; AAO50484.1; -; mRNA. DR EMBL; AY087956; AAM65504.1; -; mRNA. DR EMBL; AF466771; AAM28447.1; -; Genomic_DNA. DR EMBL; AF466772; AAM28448.1; -; Genomic_DNA. DR EMBL; AF466773; AAM28449.1; -; Genomic_DNA. DR EMBL; AF466774; AAM28450.1; -; Genomic_DNA. DR EMBL; AF466775; AAM28451.1; -; Genomic_DNA. DR EMBL; AF466776; AAM28452.1; -; Genomic_DNA. DR EMBL; AF466777; AAM28453.1; -; Genomic_DNA. DR EMBL; AF466778; AAM28454.1; -; Genomic_DNA. DR EMBL; AF466779; AAM28455.1; -; Genomic_DNA. DR EMBL; AF466780; AAM28456.1; -; Genomic_DNA. DR EMBL; AF466781; AAM28457.1; -; Genomic_DNA. DR EMBL; AF466782; AAM28458.1; -; Genomic_DNA. DR EMBL; AF466783; AAM28459.1; -; Genomic_DNA. DR EMBL; AF466784; AAM28460.1; -; Genomic_DNA. DR EMBL; AF466785; AAM28461.1; -; Genomic_DNA. DR IPI; IPI00543034; -. DR PIR; S27109; S27109. DR RefSeq; NP_177074.1; -. DR UniGene; At.10200; -. DR UniGene; At.24302; -. DR HSSP; Q02078; 1EGW. DR IntAct; P35631; 25. DR STRING; P35631; -. DR GeneID; 843244; -. DR GenomeReviews; CT485782_GR; AT1G69120. DR KEGG; ath:AT1G69120; -. DR NMPDR; fig|3702.1.peg.6371; -. DR GeneFarm; 3482; 348. DR TAIR; At1g69120; -. DR OMA; CMEKILE; -. DR Genevestigator; P35631; -. DR GermOnline; AT1G69120; Arabidopsis thaliana. DR GO; GO:0005634; C:nucleus; IDA:TAIR. DR GO; GO:0046982; F:protein heterodimerization activity; IPI:TAIR. DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro. DR GO; GO:0016563; F:transcription activator activity; IEP:TAIR. DR GO; GO:0003700; F:transcription factor activity; IEA:InterPro. DR GO; GO:0009908; P:flower development; IMP:TAIR. DR GO; GO:0010076; P:maintenance of floral meristem identity; IGI:TAIR. DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR. DR GO; GO:0045941; P:positive regulation of transcription; IGI:TAIR. DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro. DR GO; GO:0006350; P:transcription; IEA:UniProtKB-KW. DR InterPro; IPR002487; TF_Kbox. DR InterPro; IPR002100; TF_MADSbox. DR Pfam; PF01486; K-box; 1. DR Pfam; PF00319; SRF-TF; 1. DR PRINTS; PR00404; MADSDOMAIN. DR SMART; SM00432; MADS; 1. DR PROSITE; PS51297; K_BOX; 1. DR PROSITE; PS00350; MADS_BOX_1; 1. DR PROSITE; PS50066; MADS_BOX_2; 1. PE 1: Evidence at protein level; KW Activator; Coiled coil; Complete proteome; Developmental protein; KW Differentiation; DNA-binding; Flowering; Nucleus; Polymorphism; KW Transcription; Transcription regulation. FT CHAIN 1 256 Floral homeotic protein APETALA 1. FT /FTId=PRO_0000199451. FT DOMAIN 3 57 MADS-box. FT DOMAIN 88 178 K-box. FT COILED 88 185 Potential. FT VARIANT 85 85 S -> P (in strain: cv. Chi-1). FT VARIANT 93 93 M -> T (in strain: cv. Bla-1). FT VARIANT 99 99 K -> R (in strain: cv. Chi-1). FT VARIANT 100 100 A -> T (in strain: cv. Landsberg erecta). FT VARIANT 120 120 M -> V (in strain: cv. Jl-1). FT VARIANT 125 125 L -> P (in strain: cv. Bla-1). FT VARIANT 166 166 S -> G (in strain: cv. Jl-1). FT VARIANT 212 212 L -> P (in strain: cv. Chi-1). FT VARIANT 233 233 A -> V (in strain: cv. Co-1). FT CONFLICT 184 184 E -> G (in Ref. 5; AAM28458). FT CONFLICT 189 189 Q -> H (in Ref. 4; AAM65504). FT CONFLICT 236 236 Missing (in Ref. 1; CAA78909). SQ SEQUENCE 256 AA; 30182 MW; 01E26DE18CE3478C CRC64; MGRGRVQLKR IENKINRQVT FSKRRAGLLK KAHEISVLCD AEVALVVFSH KGKLFEYSTD SCMEKILERY ERYSYAERQL IAPESDVNTN WSMEYNRLKA KIELLERNQR HYLGEDLQAM SPKELQNLEQ QLDTALKHIR TRKNQLMYES INELQKKEKA IQEQNSMLSK QIKEREKILR AQQEQWDQQN QGHNMPPPLP PQQHQIQHPY MLSHQPSPFL NMGGLYQEDD PMAMRRNDLE LTLEPVYNCN LGCFAA //