ID AP1_ARATH Reviewed; 256 AA. AC P35631; Q680D4; Q8LA98; Q9LQA8; DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot. DT 20-JUN-2002, sequence version 2. DT 03-MAY-2023, entry version 179. DE RecName: Full=Floral homeotic protein APETALA 1; DE AltName: Full=Agamous-like MADS-box protein AGL7; GN Name=AP1; Synonyms=AGL7; OrderedLocusNames=At1g69120; ORFNames=F4N2.9; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Landsberg erecta; RX PubMed=1359429; DOI=10.1038/360273a0; RA Mandel M.A., Gustafson-Brown C., Savidge B., Yanofsky M.F.; RT "Molecular characterization of the Arabidopsis floral homeotic gene RT APETALA1."; RL Nature 360:273-277(1992). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., RA Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."; RL Nature 408:816-820(2000). RN [3] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., RA Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K., RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., RA Shinozaki K.; RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."; RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [7] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-251, AND VARIANTS. RC STRAIN=cv. Bla-1, cv. Bretagny, cv. Bs-1, cv. Bu-0, cv. Bu-2, cv. Chi-1, RC cv. Co-1, cv. Columbia, cv. Cvi-0, cv. Gr-3, cv. Ita-0, cv. Jl-1, RC cv. Kas-1, cv. Kent, cv. Landsberg erecta, cv. Li-3, cv. Li-8, cv. Lisse, RC and cv. Wassilewskija; RX PubMed=11973317; DOI=10.1093/genetics/160.4.1641; RA Olsen K.M., Womack A., Garrett A.R., Suddith J.I., Purugganan M.D.; RT "Contrasting evolutionary forces in the Arabidopsis thaliana floral RT developmental pathway."; RL Genetics 160:1641-1650(2002). RN [8] RP FUNCTION. RA Bowman J.L., Alvarez J., Weigel D., Meyerowitz E.M., Smyth D.R.; RT "Control of flower development in Arabidopsis thaliana by APETALA1 and RT interacting genes."; RL Development 119:721-743(1993). RN [9] RP FUNCTION. RX PubMed=17794879; DOI=10.1126/science.261.5129.1723; RA Weigel D., Meyerowitz E.M.; RT "Activation of floral homeotic genes in Arabidopsis."; RL Science 261:1723-1726(1993). RN [10] RP CHARACTERIZATION. RX PubMed=8643482; DOI=10.1073/pnas.93.10.4793; RA Riechmann J.L., Krizek B.A., Meyerowitz E.M.; RT "Dimerization specificity of Arabidopsis MADS domain homeotic proteins RT APETALA1, APETALA3, PISTILLATA, and AGAMOUS."; RL Proc. Natl. Acad. Sci. U.S.A. 93:4793-4798(1996). RN [11] RP INDUCTION. RX PubMed=9783581; DOI=10.1038/26903; RA Parcy F., Nilsson O., Busch M.A., Lee I., Weigel D.; RT "A genetic framework for floral patterning."; RL Nature 395:561-566(1998). RN [12] RP FUNCTION. RX PubMed=11283333; DOI=10.1105/tpc.13.4.739; RA Ng M., Yanofsky M.F.; RT "Activation of the Arabidopsis B class homeotic genes by APETALA1."; RL Plant Cell 13:739-753(2001). RN [13] RP CHARACTERIZATION. RX PubMed=11206550; DOI=10.1038/35054083; RA Honma T., Goto K.; RT "Complexes of MADS-box proteins are sufficient to convert leaves into RT floral organs."; RL Nature 409:525-529(2001). RN [14] RP INTERACTION WITH TT16/AGL32. RX PubMed=16080001; DOI=10.1007/s00438-005-0010-y; RA Kaufmann K., Anfang N., Saedler H., Theissen G.; RT "Mutant analysis, protein-protein interactions and subcellular localization RT of the Arabidopsis B sister (ABS) protein."; RL Mol. Genet. Genomics 274:103-118(2005). RN [15] RP INTERACTION WITH AGL15 AND AGL16. RX PubMed=15805477; DOI=10.1105/tpc.105.031831; RA de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R., RA Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B., RA Angenent G.C.; RT "Comprehensive interaction map of the Arabidopsis MADS Box transcription RT factors."; RL Plant Cell 17:1424-1433(2005). RN [16] RP INTERACTION WITH AGL24; SVP AND SEU-LUG. RX PubMed=16679456; DOI=10.1105/tpc.106.041798; RA Gregis V., Sessa A., Colombo L., Kater M.M.; RT "AGL24, SHORT VEGETATIVE PHASE, and APETALA1 redundantly control AGAMOUS RT during early stages of flower development in Arabidopsis."; RL Plant Cell 18:1373-1382(2006). RN [17] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=17428825; DOI=10.1242/dev.003103; RA Liu C., Zhou J., Bracha-Drori K., Yalovsky S., Ito T., Yu H.; RT "Specification of Arabidopsis floral meristem identity by repression of RT flowering time genes."; RL Development 134:1901-1910(2007). RN [18] RP FUNCTION. RX PubMed=19656343; DOI=10.1111/j.1365-313x.2009.03985.x; RA Gregis V., Sessa A., Dorca-Fornell C., Kater M.M.; RT "The Arabidopsis floral meristem identity genes AP1, AGL24 and SVP directly RT repress class B and C floral homeotic genes."; RL Plant J. 60:626-637(2009). CC -!- FUNCTION: Transcription factor that promotes early floral meristem CC identity in synergy with LEAFY. Is required subsequently for the CC transition of an inflorescence meristem into a floral meristem. Is CC indispensable for normal development of sepals and petals in flowers. CC Regulates positively the B class homeotic proteins APETALA3 and CC PISTILLATA with the cooperation of LEAFY and UFO. Interacts with CC SEPALLATA3 or AP3/PI heterodimer to form complexes that could be CC involved in genes regulation during floral meristem development. CC Regulates positively AGAMOUS in cooperation with LEAFY. Displays a CC redundant function with CAULIFLOWER in the up-regulation of LEAFY. CC Together with AGL24 and SVP, controls the identity of the floral CC meristem and regulates expression of class B, C and E genes. Represses CC flowering time genes AGL24, SVP and SOC1 in emerging floral meristems. CC {ECO:0000269|PubMed:11283333, ECO:0000269|PubMed:17428825, CC ECO:0000269|PubMed:17794879, ECO:0000269|PubMed:19656343, CC ECO:0000269|Ref.8}. CC -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences. CC Heterodimer with SEP3, AP1 and SVP. Binds AP3/PI to form a ternary CC complex. Interacts with the SEU-LUG corepressor complex when complexed CC to AGL24 or SVP. Interacts with AGL15 and AGL16 (PubMed:15805477, CC PubMed:16679456). Interacts with TT16/AGL32 (PubMed:16080001). CC {ECO:0000269|PubMed:15805477, ECO:0000269|PubMed:16080001, CC ECO:0000269|PubMed:16679456}. CC -!- INTERACTION: CC P35631; Q9SZJ6: AGL21; NbExp=4; IntAct=EBI-592003, EBI-621986; CC P35631; O82794: AGL24; NbExp=4; IntAct=EBI-592003, EBI-592083; CC P35631; P29382: SEP1; NbExp=4; IntAct=EBI-592003, EBI-632935; CC P35631; O22456: SEP3; NbExp=4; IntAct=EBI-592003, EBI-592020; CC P35631; Q8W234: SEU; NbExp=2; IntAct=EBI-592003, EBI-1771131; CC P35631; O64645: SOC1; NbExp=5; IntAct=EBI-592003, EBI-592041; CC P35631; Q9FVC1: SVP; NbExp=5; IntAct=EBI-592003, EBI-592058; CC P35631; Q93Z00: TCP14; NbExp=3; IntAct=EBI-592003, EBI-4424563; CC P35631; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-592003, EBI-4426144; CC P35631; Q8LPR5: TCP4; NbExp=3; IntAct=EBI-592003, EBI-15192325; CC P35631; Q2NJQ2: AYWB_224; Xeno; NbExp=3; IntAct=EBI-592003, EBI-16100490; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- TISSUE SPECIFICITY: Expressed in young flower primordia, later becomes CC localized to sepals and petals. CC -!- DEVELOPMENTAL STAGE: Expressed at an early stage of floral initiation. CC -!- INDUCTION: Negatively regulated by TFL1 and by the C class floral CC homeotic protein AGAMOUS. Positively regulated by CAULIFLOWER. CC {ECO:0000269|PubMed:9783581}. CC -!- DISRUPTION PHENOTYPE: Partial conversion of flowers into shoots and a CC disruption of sepal and petal development. CC {ECO:0000269|PubMed:17428825}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z16421; CAA78909.1; -; mRNA. DR EMBL; AC008262; AAF27070.1; -; Genomic_DNA. DR EMBL; CP002684; AEE34887.1; -; Genomic_DNA. DR EMBL; BT004113; AAO42136.1; -; mRNA. DR EMBL; BT004951; AAO50484.1; -; mRNA. DR EMBL; AK175933; BAD43696.1; -; mRNA. DR EMBL; AY087956; AAM65504.1; -; mRNA. DR EMBL; AF466771; AAM28447.1; -; Genomic_DNA. DR EMBL; AF466772; AAM28448.1; -; Genomic_DNA. DR EMBL; AF466773; AAM28449.1; -; Genomic_DNA. DR EMBL; AF466774; AAM28450.1; -; Genomic_DNA. DR EMBL; AF466775; AAM28451.1; -; Genomic_DNA. DR EMBL; AF466776; AAM28452.1; -; Genomic_DNA. DR EMBL; AF466777; AAM28453.1; -; Genomic_DNA. DR EMBL; AF466778; AAM28454.1; -; Genomic_DNA. DR EMBL; AF466779; AAM28455.1; -; Genomic_DNA. DR EMBL; AF466780; AAM28456.1; -; Genomic_DNA. DR EMBL; AF466781; AAM28457.1; -; Genomic_DNA. DR EMBL; AF466782; AAM28458.1; -; Genomic_DNA. DR EMBL; AF466783; AAM28459.1; -; Genomic_DNA. DR EMBL; AF466784; AAM28460.1; -; Genomic_DNA. DR EMBL; AF466785; AAM28461.1; -; Genomic_DNA. DR PIR; S27109; S27109. DR RefSeq; NP_177074.1; NM_105581.3. DR AlphaFoldDB; P35631; -. DR SMR; P35631; -. DR BioGRID; 28465; 57. DR DIP; DIP-33763N; -. DR IntAct; P35631; 42. DR STRING; 3702.AT1G69120.1; -. DR PaxDb; P35631; -. DR ProteomicsDB; 244444; -. DR EnsemblPlants; AT1G69120.1; AT1G69120.1; AT1G69120. DR GeneID; 843244; -. DR Gramene; AT1G69120.1; AT1G69120.1; AT1G69120. DR KEGG; ath:AT1G69120; -. DR Araport; AT1G69120; -. DR TAIR; locus:2033273; AT1G69120. DR eggNOG; KOG0014; Eukaryota. DR HOGENOM; CLU_053053_0_2_1; -. DR InParanoid; P35631; -. DR OrthoDB; 448298at2759; -. DR PhylomeDB; P35631; -. DR PRO; PR:P35631; -. DR Proteomes; UP000006548; Chromosome 1. DR ExpressionAtlas; P35631; baseline and differential. DR Genevisible; P35631; AT. DR GO; GO:0005634; C:nucleus; IDA:TAIR. DR GO; GO:0003677; F:DNA binding; IDA:TAIR. DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR. DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. DR GO; GO:0046982; F:protein heterodimerization activity; IPI:TAIR. DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central. DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR. DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW. DR GO; GO:0010582; P:floral meristem determinacy; IGI:TAIR. DR GO; GO:0009908; P:flower development; IMP:TAIR. DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR. DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IDA:TAIR. DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro. DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central. DR CDD; cd00265; MADS_MEF2_like; 1. DR Gene3D; 3.40.1810.10; Transcription factor, MADS-box; 1. DR InterPro; IPR033896; MADS_MEF2-like. DR InterPro; IPR002487; TF_Kbox. DR InterPro; IPR002100; TF_MADSbox. DR InterPro; IPR036879; TF_MADSbox_sf. DR PANTHER; PTHR48019; SERUM RESPONSE FACTOR HOMOLOG; 1. DR PANTHER; PTHR48019:SF79; TRUNCATED TRANSCRIPTION FACTOR CAULIFLOWER A; 1. DR Pfam; PF01486; K-box; 1. DR Pfam; PF00319; SRF-TF; 1. DR PRINTS; PR00404; MADSDOMAIN. DR SMART; SM00432; MADS; 1. DR SUPFAM; SSF55455; SRF-like; 1. DR PROSITE; PS51297; K_BOX; 1. DR PROSITE; PS00350; MADS_BOX_1; 1. DR PROSITE; PS50066; MADS_BOX_2; 1. PE 1: Evidence at protein level; KW Activator; Coiled coil; Developmental protein; Differentiation; KW DNA-binding; Flowering; Nucleus; Reference proteome; Transcription; KW Transcription regulation. FT CHAIN 1..256 FT /note="Floral homeotic protein APETALA 1" FT /id="PRO_0000199451" FT DOMAIN 1..61 FT /note="MADS-box" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251" FT DOMAIN 88..178 FT /note="K-box" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629" FT COILED 88..185 FT /evidence="ECO:0000255" FT VARIANT 85 FT /note="S -> P (in strain: cv. Chi-1)" FT VARIANT 93 FT /note="M -> T (in strain: cv. Bla-1)" FT VARIANT 99 FT /note="K -> R (in strain: cv. Chi-1)" FT VARIANT 100 FT /note="A -> T (in strain: cv. Landsberg erecta)" FT VARIANT 120 FT /note="M -> V (in strain: cv. Jl-1)" FT VARIANT 125 FT /note="L -> P (in strain: cv. Bla-1)" FT VARIANT 166 FT /note="S -> G (in strain: cv. Jl-1)" FT VARIANT 212 FT /note="L -> P (in strain: cv. Chi-1)" FT VARIANT 233 FT /note="A -> V (in strain: cv. Co-1)" FT CONFLICT 139 FT /note="I -> M (in Ref. 5; BAD43696)" FT /evidence="ECO:0000305" FT CONFLICT 184 FT /note="E -> G (in Ref. 7; AAM28458)" FT /evidence="ECO:0000305" FT CONFLICT 189 FT /note="Q -> H (in Ref. 6; AAM65504)" FT /evidence="ECO:0000305" FT CONFLICT 236 FT /note="Missing (in Ref. 1; CAA78909)" FT /evidence="ECO:0000305" SQ SEQUENCE 256 AA; 30182 MW; 01E26DE18CE3478C CRC64; MGRGRVQLKR IENKINRQVT FSKRRAGLLK KAHEISVLCD AEVALVVFSH KGKLFEYSTD SCMEKILERY ERYSYAERQL IAPESDVNTN WSMEYNRLKA KIELLERNQR HYLGEDLQAM SPKELQNLEQ QLDTALKHIR TRKNQLMYES INELQKKEKA IQEQNSMLSK QIKEREKILR AQQEQWDQQN QGHNMPPPLP PQQHQIQHPY MLSHQPSPFL NMGGLYQEDD PMAMRRNDLE LTLEPVYNCN LGCFAA //