ID AP1_ARATH Reviewed; 256 AA. AC P35631; Q680D4; Q8LA98; Q9LQA8; DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot. DT 20-JUN-2002, sequence version 2. DT 11-JUL-2012, entry version 113. DE RecName: Full=Floral homeotic protein APETALA 1; DE AltName: Full=Agamous-like MADS-box protein AGL7; GN Name=AP1; Synonyms=AGL7; OrderedLocusNames=At1g69120; ORFNames=F4N2.9; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Landsberg erecta; RX MEDLINE=93063372; PubMed=1359429; DOI=10.1038/360273a0; RA Mandel M.A., Gustafson-Brown C., Savidge B., Yanofsky M.F.; RT "Molecular characterization of the Arabidopsis floral homeotic gene RT APETALA1."; RL Nature 360:273-277(1992). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., RA White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., RA Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., RA Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., RA Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., RA Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., RA Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., RA Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., RA Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., RA Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., RA Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., RA Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., RA Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., RA Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., RA Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis RT thaliana."; RL Nature 408:816-820(2000). RN [3] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Resource (TAIR); RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K., RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., RA Hayashizaki Y., Shinozaki K.; RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."; RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [7] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-251, AND VARIANTS. RC STRAIN=cv. Bla-1, cv. Bretagny, cv. Bs-1, cv. Bu-0, cv. Bu-2, RC cv. Chi-1, cv. Co-1, cv. Columbia, cv. Cvi-0, cv. Gr-3, cv. Ita-0, RC cv. Jl-1, cv. Kas-1, cv. Kent, cv. Landsberg erecta, cv. Li-3, RC cv. Li-8, cv. Lisse, and cv. Wassilewskija; RX MEDLINE=21969421; PubMed=11973317; RA Olsen K.M., Womack A., Garrett A.R., Suddith J.I., Purugganan M.D.; RT "Contrasting evolutionary forces in the Arabidopsis thaliana floral RT developmental pathway."; RL Genetics 160:1641-1650(2002). RN [8] RP FUNCTION. RA Bowman J.L., Alvarez J., Weigel D., Meyerowitz E.M., Smyth D.R.; RT "Control of flower development in Arabidopsis thaliana by APETALA1 and RT interacting genes."; RL Development 119:721-743(1993). RN [9] RP FUNCTION. RX PubMed=17794879; DOI=10.1126/science.261.5129.1723; RA Weigel D., Meyerowitz E.M.; RT "Activation of floral homeotic genes in Arabidopsis."; RL Science 261:1723-1726(1993). RN [10] RP CHARACTERIZATION. RX MEDLINE=96209811; PubMed=8643482; DOI=10.1073/pnas.93.10.4793; RA Riechmann J.L., Krizek B.A., Meyerowitz E.M.; RT "Dimerization specificity of Arabidopsis MADS domain homeotic proteins RT APETALA1, APETALA3, PISTILLATA, and AGAMOUS."; RL Proc. Natl. Acad. Sci. U.S.A. 93:4793-4798(1996). RN [11] RP GENETIC REGULATION. RX PubMed=9783581; DOI=10.1038/26903; RA Parcy F., Nilsson O., Busch M.A., Lee I., Weigel D.; RT "A genetic framework for floral patterning."; RL Nature 395:561-566(1998). RN [12] RP FUNCTION. RX MEDLINE=21178805; PubMed=11283333; DOI=10.1105/tpc.13.4.739; RA Ng M., Yanofsky M.F.; RT "Activation of the Arabidopsis B class homeotic genes by APETALA1."; RL Plant Cell 13:739-753(2001). RN [13] RP CHARACTERIZATION. RX MEDLINE=21074934; PubMed=11206550; DOI=10.1038/35054083; RA Honma T., Goto K.; RT "Complexes of MADS-box proteins are sufficient to convert leaves into RT floral organs."; RL Nature 409:525-529(2001). RN [14] RP INTERACTION WITH AGL15 AND AGL16. RX PubMed=15805477; DOI=10.1105/tpc.105.031831; RA de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R., RA Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B., RA Angenent G.C.; RT "Comprehensive interaction map of the Arabidopsis MADS Box RT transcription factors."; RL Plant Cell 17:1424-1433(2005). RN [15] RP INTERACTION WITH AGL24; SVP AND SEU-LUG. RX PubMed=16679456; DOI=10.1105/tpc.106.041798; RA Gregis V., Sessa A., Colombo L., Kater M.M.; RT "AGL24, SHORT VEGETATIVE PHASE, and APETALA1 redundantly control RT AGAMOUS during early stages of flower development in Arabidopsis."; RL Plant Cell 18:1373-1382(2006). RN [16] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=17428825; DOI=10.1242/dev.003103; RA Liu C., Zhou J., Bracha-Drori K., Yalovsky S., Ito T., Yu H.; RT "Specification of Arabidopsis floral meristem identity by repression RT of flowering time genes."; RL Development 134:1901-1910(2007). RN [17] RP FUNCTION. RX PubMed=19656343; DOI=10.1111/j.1365-313X.2009.03985.x; RA Gregis V., Sessa A., Dorca-Fornell C., Kater M.M.; RT "The Arabidopsis floral meristem identity genes AP1, AGL24 and SVP RT directly repress class B and C floral homeotic genes."; RL Plant J. 60:626-637(2009). CC -!- FUNCTION: Transcription factor that promotes early floral meristem CC identity in synergy with LEAFY. Is required subsequently for the CC transition of an inflorescence meristem into a floral meristem. Is CC indispensable for normal development of sepals and petals in CC flowers. Regulates positively the B class homeotic proteins CC APETALA3 and PISTILLATA with the cooperation of LEAFY and UFO. CC Interacts with SEPALLATA3 or AP3/PI heterodimer to form complexes CC that could be involved in genes regulation during floral meristem CC development. Regulates positively AGAMOUS in cooperation with CC LEAFY. Displays a redundant function with CAULIFLOWER in the up- CC regulation of LEAFY. Together with AGL24 and SVP, controls the CC identity of the floral meristem and regulates expression of class CC B, C and E genes. Represses flowering time genes AGL24, SVP and CC SOC1 in emerging floral meristems. CC -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences. CC Heterodimer with SEP3, AP1 and SVP. Binds AP3/PI to form a ternary CC complex. Interacts with the SEU-LUG corepressor complex when CC complexed to AGL24 or SVP. Interacts with AGL15 and AGL16. CC -!- INTERACTION: CC O82794:AGL24; NbExp=4; IntAct=EBI-592003, EBI-592083; CC O22456:SEP3; NbExp=4; IntAct=EBI-592003, EBI-592020; CC Q8W234:SEU; NbExp=2; IntAct=EBI-592003, EBI-1771131; CC O64645:SOC1; NbExp=5; IntAct=EBI-592003, EBI-592041; CC Q9FVC1:SVP; NbExp=3; IntAct=EBI-592003, EBI-592058; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- TISSUE SPECIFICITY: Expressed in young flower primordia, later CC becomes localized to sepals and petals. CC -!- DEVELOPMENTAL STAGE: Expressed at an early stage of floral CC initiation. CC -!- INDUCTION: Negatively regulated by TFL1 and by the C class floral CC homeotic protein AGAMOUS. Positively regulated by CAULIFLOWER. CC -!- DISRUPTION PHENOTYPE: Partial conversion of flowers into shoots CC and a disruption of sepal and petal development. CC -!- SIMILARITY: Contains 1 K-box domain. CC -!- SIMILARITY: Contains 1 MADS-box domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z16421; CAA78909.1; -; mRNA. DR EMBL; AC008262; AAF27070.1; -; Genomic_DNA. DR EMBL; CP002684; AEE34887.1; -; Genomic_DNA. DR EMBL; BT004113; AAO42136.1; -; mRNA. DR EMBL; BT004951; AAO50484.1; -; mRNA. DR EMBL; AK175933; BAD43696.1; -; mRNA. DR EMBL; AY087956; AAM65504.1; -; mRNA. DR EMBL; AF466771; AAM28447.1; -; Genomic_DNA. DR EMBL; AF466772; AAM28448.1; -; Genomic_DNA. DR EMBL; AF466773; AAM28449.1; -; Genomic_DNA. DR EMBL; AF466774; AAM28450.1; -; Genomic_DNA. DR EMBL; AF466775; AAM28451.1; -; Genomic_DNA. DR EMBL; AF466776; AAM28452.1; -; Genomic_DNA. DR EMBL; AF466777; AAM28453.1; -; Genomic_DNA. DR EMBL; AF466778; AAM28454.1; -; Genomic_DNA. DR EMBL; AF466779; AAM28455.1; -; Genomic_DNA. DR EMBL; AF466780; AAM28456.1; -; Genomic_DNA. DR EMBL; AF466781; AAM28457.1; -; Genomic_DNA. DR EMBL; AF466782; AAM28458.1; -; Genomic_DNA. DR EMBL; AF466783; AAM28459.1; -; Genomic_DNA. DR EMBL; AF466784; AAM28460.1; -; Genomic_DNA. DR EMBL; AF466785; AAM28461.1; -; Genomic_DNA. DR IPI; IPI00543034; -. DR PIR; S27109; S27109. DR RefSeq; NP_177074.1; NM_105581.2. DR UniGene; At.10200; -. DR UniGene; At.24302; -. DR ProteinModelPortal; P35631; -. DR SMR; P35631; 2-77. DR IntAct; P35631; 22. DR STRING; P35631; -. DR EnsemblPlants; AT1G69120; AT1G69120; AT1G69120. DR GeneID; 843244; -. DR GenomeReviews; CT485782_GR; AT1G69120. DR KEGG; ath:AT1G69120; -. DR GeneFarm; 3482; 348. DR TAIR; At1g69120; -. DR eggNOG; COG5068; -. DR HOGENOM; HOG000155301; -. DR InParanoid; P35631; -. DR KO; K09264; -. DR OMA; LMYESIN; -. DR PhylomeDB; P35631; -. DR ProtClustDB; CLSN2682513; -. DR Genevestigator; P35631; -. DR GermOnline; AT1G69120; Arabidopsis thaliana. DR GO; GO:0005634; C:nucleus; IDA:TAIR. DR GO; GO:0003677; F:DNA binding; IDA:TAIR. DR GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro. DR GO; GO:0048830; P:adventitious root development; IEA:EnsemblPlants/Gramene. DR GO; GO:0010582; P:floral meristem determinacy; IGI:TAIR. DR GO; GO:0010076; P:maintenance of floral meristem identity; IGI:TAIR. DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR. DR GO; GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:TAIR. DR GO; GO:0010093; P:specification of floral organ identity; IEA:EnsemblPlants/Gramene. DR GO; GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. DR InterPro; IPR002487; TF_Kbox. DR InterPro; IPR002100; TF_MADSbox. DR Pfam; PF01486; K-box; 1. DR Pfam; PF00319; SRF-TF; 1. DR PRINTS; PR00404; MADSDOMAIN. DR SMART; SM00432; MADS; 1. DR SUPFAM; SSF55455; TF_MADSbox; 1. DR PROSITE; PS51297; K_BOX; 1. DR PROSITE; PS00350; MADS_BOX_1; 1. DR PROSITE; PS50066; MADS_BOX_2; 1. PE 1: Evidence at protein level; KW Activator; Coiled coil; Complete proteome; Developmental protein; KW Differentiation; DNA-binding; Flowering; Nucleus; Polymorphism; KW Reference proteome; Transcription; Transcription regulation. FT CHAIN 1 256 Floral homeotic protein APETALA 1. FT /FTId=PRO_0000199451. FT DOMAIN 1 61 MADS-box. FT DOMAIN 88 178 K-box. FT COILED 88 185 Potential. FT VARIANT 85 85 S -> P (in strain: cv. Chi-1). FT VARIANT 93 93 M -> T (in strain: cv. Bla-1). FT VARIANT 99 99 K -> R (in strain: cv. Chi-1). FT VARIANT 100 100 A -> T (in strain: cv. Landsberg erecta). FT VARIANT 120 120 M -> V (in strain: cv. Jl-1). FT VARIANT 125 125 L -> P (in strain: cv. Bla-1). FT VARIANT 166 166 S -> G (in strain: cv. Jl-1). FT VARIANT 212 212 L -> P (in strain: cv. Chi-1). FT VARIANT 233 233 A -> V (in strain: cv. Co-1). FT CONFLICT 139 139 I -> M (in Ref. 5; BAD43696). FT CONFLICT 184 184 E -> G (in Ref. 7; AAM28458). FT CONFLICT 189 189 Q -> H (in Ref. 6; AAM65504). FT CONFLICT 236 236 Missing (in Ref. 1; CAA78909). SQ SEQUENCE 256 AA; 30182 MW; 01E26DE18CE3478C CRC64; MGRGRVQLKR IENKINRQVT FSKRRAGLLK KAHEISVLCD AEVALVVFSH KGKLFEYSTD SCMEKILERY ERYSYAERQL IAPESDVNTN WSMEYNRLKA KIELLERNQR HYLGEDLQAM SPKELQNLEQ QLDTALKHIR TRKNQLMYES INELQKKEKA IQEQNSMLSK QIKEREKILR AQQEQWDQQN QGHNMPPPLP PQQHQIQHPY MLSHQPSPFL NMGGLYQEDD PMAMRRNDLE LTLEPVYNCN LGCFAA //