ID MTSS1_CAEEL Reviewed; 170 AA. AC P34496; DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1995, sequence version 2. DT 07-APR-2021, entry version 133. DE RecName: Full=Single-stranded DNA-binding protein, mitochondrial; DE Flags: Precursor; GN Name=mtss-1 {ECO:0000312|WormBase:PAR2.1}; GN ORFNames=PAR2.1 {ECO:0000312|WormBase:PAR2.1}; OS Caenorhabditis elegans. OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; OC Caenorhabditis. OX NCBI_TaxID=6239; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Bristol N2; RX PubMed=7906398; DOI=10.1038/368032a0; RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A., RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., RA Wilkinson-Sproat J., Wohldman P.; RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C. RT elegans."; RL Nature 368:32-38(1994). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Bristol N2; RX PubMed=9851916; DOI=10.1126/science.282.5396.2012; RG The C. elegans sequencing consortium; RT "Genome sequence of the nematode C. elegans: a platform for investigating RT biology."; RL Science 282:2012-2018(1998). RN [3] RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION RP PHENOTYPE. RX PubMed=17900564; DOI=10.1016/j.yexcr.2007.08.015; RA Sugimoto T., Mori C., Takanami T., Sasagawa Y., Saito R., Ichiishi E., RA Higashitani A.; RT "Caenorhabditis elegans par2.1/mtssb-1 is essential for mitochondrial DNA RT replication and its defect causes comprehensive transcriptional alterations RT including a hypoxia response."; RL Exp. Cell Res. 314:103-114(2008). RN [4] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=20580819; DOI=10.1016/j.bbadis.2010.05.007; RA Addo M.G., Cossard R., Pichard D., Obiri-Danso K., Rotig A., Delahodde A.; RT "Caenorhabditis elegans, a pluricellular model organism to screen new genes RT involved in mitochondrial genome maintenance."; RL Biochim. Biophys. Acta 1802:765-773(2010). RN [5] RP INTERACTION WITH NMAD-1. RX PubMed=31283754; DOI=10.1371/journal.pgen.1008252; RA Wang S.Y., Mao H., Shibuya H., Uzawa S., O'Brown Z.K., Wesenberg S., RA Shin N., Saito T.T., Gao J., Meyer B.J., Colaiacovo M.P., Greer E.L.; RT "The demethylase NMAD-1 regulates DNA replication and repair in the RT Caenorhabditis elegans germline."; RL PLoS Genet. 15:E1008252-E1008252(2019). CC -!- FUNCTION: Has a role in germline cell development and proliferation CC which affects the frequency of apoptosis. Required for mitochondrial CC DNA replication and transcription and fertility. CC {ECO:0000269|PubMed:17900564, ECO:0000269|PubMed:20580819}. CC -!- SUBUNIT: Interacts with nmad-1. {ECO:0000269|PubMed:31283754}. CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}. Mitochondrion CC matrix, mitochondrion nucleoid {ECO:0000250}. CC -!- TISSUE SPECIFICITY: Expressed in eggs. {ECO:0000269|PubMed:17900564}. CC -!- DEVELOPMENTAL STAGE: Expressed at low levels in L1 larvae and increases CC at the L3 stage to the adult stage. {ECO:0000269|PubMed:17900564}. CC -!- DISRUPTION PHENOTYPE: Reduced mitochondrial replication, CC transcriptional hypoxia response, sterility, arrest of germline cell CC proliferation, reduced frequency of apoptosis in the germline. Severe CC mitochondrial DNA depletion in somatic cells but does not result in CC major anomalies in worms development. Hypersensitive to ethidium CC bromide. {ECO:0000269|PubMed:17900564, ECO:0000269|PubMed:20580819}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BX284603; CCD72532.1; -; Genomic_DNA. DR PIR; S44857; S44857. DR RefSeq; NP_498935.1; NM_066534.3. DR SMR; P34496; -. DR BioGRID; 52193; 7. DR DIP; DIP-25867N; -. DR IntAct; P34496; 4. DR STRING; 6239.PAR2.1; -. DR EPD; P34496; -. DR PaxDb; P34496; -. DR PeptideAtlas; P34496; -. DR EnsemblMetazoa; PAR2.1.1; PAR2.1.1; WBGene00019800. DR GeneID; 187502; -. DR KEGG; cel:CELE_PAR2.1; -. DR CTD; 187502; -. DR WormBase; PAR2.1; CE00768; WBGene00019800; mtss-1. DR eggNOG; KOG1653; Eukaryota. DR GeneTree; ENSGT00390000002796; -. DR HOGENOM; CLU_135207_0_0_1; -. DR InParanoid; P34496; -. DR OMA; CMSLTSK; -. DR OrthoDB; 1409599at2759; -. DR PhylomeDB; P34496; -. DR PRO; PR:P34496; -. DR Proteomes; UP000001940; Chromosome III. DR Bgee; WBGene00019800; Expressed in material anatomical entity and 5 other tissues. DR GO; GO:0042645; C:mitochondrial nucleoid; IBA:GO_Central. DR GO; GO:0009295; C:nucleoid; IBA:GO_Central. DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central. DR GO; GO:0006264; P:mitochondrial DNA replication; IBA:GO_Central. DR GO; GO:0051096; P:positive regulation of helicase activity; IBA:GO_Central. DR CDD; cd04496; SSB_OBF; 1. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR000424; Primosome_PriB/ssb. DR InterPro; IPR011344; ssDNA-bd. DR PANTHER; PTHR10302; PTHR10302; 1. DR Pfam; PF00436; SSB; 1. DR SUPFAM; SSF50249; SSF50249; 1. DR TIGRFAMs; TIGR00621; ssb; 1. DR PROSITE; PS50935; SSB; 1. PE 1: Evidence at protein level; KW DNA replication; DNA-binding; Mitochondrion; Mitochondrion nucleoid; KW Reference proteome; Transit peptide. FT TRANSIT 1..30 FT /note="Mitochondrion" FT /evidence="ECO:0000255" FT CHAIN 31..170 FT /note="Single-stranded DNA-binding protein, mitochondrial" FT /id="PRO_0000095993" FT DOMAIN 54..160 FT /note="SSB" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00252" SQ SEQUENCE 170 AA; 19182 MW; D1FA3DEA8F94ABE1 CRC64; MLRSLSTISK STVRCMSLTS KMAAEQPSKQ EVDDLFAEKP QHHNPEQRRH AYSVNKVELV GGVALDPLYK TGRNGKPYLI FNIITNSYFK QQDGTTLDQT ERHAVSVFGK QAEILSKTIK KGSRLMVQGR LHYSGGQKDE QGNRTQRNTY IIAQTVQPLA RAARENPDQH //