ID TGL1_YEAST STANDARD; PRT; 548 AA. AC P34163; DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1994, sequence version 1. DT 25-JUL-2006, entry version 45. DE Triglyceride lipase-cholesterol esterase (EC 3.1.1.-). GN Name=TGL1; OrderedLocusNames=YKL140W; ORFNames=YKL5; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=92245761; PubMed=1574929; RA Abraham P.R., Mulder A., Van'T Riet J., Planta R.J., Raue H.A.; RT "Molecular cloning and physical analysis of an 8.2 kb segment of RT chromosome XI of Saccharomyces cerevisiae reveals five tightly linked RT genes."; RL Yeast 8:227-238(1992). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 96604 / S288c / FY1679; RX MEDLINE=94255012; PubMed=8196765; DOI=10.1038/369371a0; RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., RA Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A., RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., RA Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., RA Zimmermann J., Haasemann M., Becker I., Mewes H.-W.; RT "Complete DNA sequence of yeast chromosome XI."; RL Nature 369:371-378(1994). RN [3] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [4] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-521 AND SER-537, AND RP MASS SPECTROMETRY. RX PubMed=15665377; DOI=10.1074/mcp.M400219-MCP200; RA Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., RA Mann M., Jensen O.N.; RT "Quantitative phosphoproteomics applied to the yeast pheromone RT signaling pathway."; RL Mol. Cell. Proteomics 4:310-327(2005). CC -!- MISCELLANEOUS: Present with 1470 molecules/cell. CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z25464; CAA80958.1; -; Genomic_DNA. DR EMBL; Z28140; CAA81981.1; -; Genomic_DNA. DR PIR; S37969; S37969. DR HSSP; P80035; 1K8Q. DR IntAct; P34163; -. DR GermOnline; 139896; -. DR Ensembl; YKL140W; Saccharomyces cerevisiae. DR GenomeReviews; Y13137_GR; YKL140W. DR SGD; S000001623; TGL1. DR BioCyc; SCER-S28-01:SCER-S28-01-003619-MONOMER; -. DR LinkHub; P34163; -. DR GO; GO:0016021; C:integral to membrane; IDA. DR GO; GO:0005811; C:lipid particle; IDA. DR GO; GO:0004771; F:sterol esterase activity; IDA. DR GO; GO:0006629; P:lipid metabolism; IMP. DR GO; GO:0016125; P:sterol metabolism; IMP. DR InterPro; IPR006693; AB_hydro_lipase. DR InterPro; IPR000073; AB_hydrolase_1. DR InterPro; IPR000379; Ser_estrs. DR Pfam; PF04083; Abhydro_lipase; 1. DR Pfam; PF00561; Abhydrolase_1; 1. KW Complete proteome; Hydrolase; Lipid degradation; Phosphorylation. FT CHAIN 1 548 Triglyceride lipase-cholesterol esterase. FT /FTId=PRO_0000090379. FT MOD_RES 521 521 Phosphoserine. FT MOD_RES 537 537 Phosphoserine. SQ SEQUENCE 548 AA; 62979 MW; 32D1F230701CB083 CRC64; MYFPFLGRLS ITDYIIVVLV YIESIISSVL KLIPQPMINL FEWLINFSTS SDDNTIEEKL RSAPTIHEMC AIFDISVEDH LVRTEDNYIL TLHRIPPISK NRFNNKVVYL HHGLLMCSDV WCCNIERHKN LPFVLHDLGY DVWMGNNRGN KYSTAHLNKP PKSNKFWDFS IDEFAFFDIP NSIEFILDIT KVDKVICIGF SQGSAQMFAA FSLSEKLNRK VSHFIAIAPA MTPKGLHNRI VDTLAKSSPG FMYLFFGRKI VLPSAVIWQR TLHPTLFNLC IDIANKILFN WKSFNILPRQ KIASYAKLYS TTSVKSIVHW FQILRSQKFQ MFEESDNMLN SLTRPYQIAN FPTRTNIKIP ILLIYGGIDS LVDIDVMKKN LPFNSVFDVK VDNYEHLDLI WGKDADTLVI AKVLRFIEFF NPGNVSVKTN QLLPSASLVE ELPSTTWKTT HPTHGLSYRT HSADRSPLSV QADEADEVHN ADNSRFLRRV FSTSAIDEDN ENEHQDDTED QIHKEQQRRL SAYLESSKDL RQLDANSSTT ALDALNKE //