ID OPRM_RAT Reviewed; 398 AA. AC P33535; Q2TV20; Q2TV21; Q4VWM5; Q4VWM7; Q4VWX7; Q4VWX8; Q62846; AC Q64064; Q64120; DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1994, sequence version 1. DT 16-NOV-2011, entry version 98. DE RecName: Full=Mu-type opioid receptor; DE Short=M-OR-1; DE Short=MOR-1; DE AltName: Full=Opioid receptor B; GN Name=Oprm1; Synonyms=Ror-b; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Rattus. OX NCBI_TaxID=10116; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RC TISSUE=Brain; RX MEDLINE=93351652; PubMed=8394245; DOI=10.1016/0014-5793(93)81011-N; RA Fukuda K., Kato S., Mori K., Nishi M., Takeshima H.; RT "Primary structures and expression from cDNAs of rat opioid receptor RT delta- and mu-subtypes."; RL FEBS Lett. 327:311-314(1993). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RC TISSUE=Brain; RX MEDLINE=94052137; PubMed=8234282; DOI=10.1073/pnas.90.21.10230; RA Wang J.-B., Imai Y., Epler M.C., Gregor P., Spivak C., Uhl G.R.; RT "Mu opiate receptor: cDNA cloning and expression."; RL Proc. Natl. Acad. Sci. U.S.A. 90:10230-10234(1993). RN [3] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RC TISSUE=Brain; RX MEDLINE=93341493; PubMed=8393525; RA Chen Y., Mestek A., Liu J., Hurley J.A., Yu L.; RT "Molecular cloning and functional expression of a mu-opioid receptor RT from rat brain."; RL Mol. Pharmacol. 44:8-12(1993). RN [4] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RC STRAIN=Sprague-Dawley; TISSUE=Brain; RA Bunzow J.R., Grandy D.K., Kelly M.; RL Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RC STRAIN=Sprague-Dawley; TISSUE=Olfactory bulb; RX MEDLINE=94059560; PubMed=8240812; DOI=10.1016/0896-6273(93)90120-G; RA Thompson R.C., Mansour A., Akil H., Watson S.J.; RT "Cloning and pharmacological characterization of a rat mu opioid RT receptor."; RL Neuron 11:903-913(1993). RN [6] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RC TISSUE=Brain; RX MEDLINE=94246380; PubMed=8189219; RA Zastawny R.L., George S.R., Nguyen T., Cheng R., Tsatsos J., RA Briones-Urbina R., O'Dowd B.F.; RT "Cloning, characterization, and distribution of a mu-opioid receptor RT in rat brain."; RL J. Neurochem. 62:2099-2105(1994). RN [7] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 5). RC STRAIN=Sprague-Dawley; RA Pan Y.-X., Xu J., Pasternak G.W.; RT "Identification and characterization of two new alternatively spliced RT variants from the rat mu opioid receptor gene, Oprm."; RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. RN [8] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 6; 7 AND 8). RC STRAIN=Sprague-Dawley; RX PubMed=15525342; DOI=10.1111/j.1471-4159.2004.02767.x; RA Pasternak D.A., Pan L., Xu J., Yu R., Xu M.M., Pasternak G.W., RA Pan Y.X.; RT "Identification of three new alternatively spliced variants of the rat RT mu opioid receptor gene: dissociation of affinity and efficacy."; RL J. Neurochem. 91:881-890(2004). RN [9] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Brown Norway; RX PubMed=15057822; DOI=10.1038/nature02426; RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., RA Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., RA Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., RA Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., RA Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., RA Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., RA Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., RA Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., RA Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., RA D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., RA Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., RA Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., RA Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., RA Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., RA Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., RA Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., RA Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., RA Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., RA Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., RA Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., RA Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., RA Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., RA Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., RA Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., RA Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., RA Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., RA Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., RA Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., RA Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., RA Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., RA Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., RA Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., RA Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., RA Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., RA Collins F.S.; RT "Genome sequence of the Brown Norway rat yields insights into RT mammalian evolution."; RL Nature 428:493-521(2004). RN [10] RP NUCLEOTIDE SEQUENCE [MRNA] OF 101-340. RC TISSUE=Macrophage; RX MEDLINE=95251654; PubMed=7733926; DOI=10.1006/bbrc.1995.1538; RA Sedqi M., Roy S., Ramakrishnan S., Elde R., Loh H.H.; RT "Complementary DNA cloning of a mu-opioid receptor from rat peritoneal RT macrophages."; RL Biochem. Biophys. Res. Commun. 209:563-574(1995). RN [11] RP NUCLEOTIDE SEQUENCE [MRNA] OF 356-398 (ISOFORM 4). RX MEDLINE=95172221; PubMed=7532594; DOI=10.1016/0014-5793(95)00028-8; RA Zimprich A., Simon T., Hoellt V.; RT "Cloning and expression of an isoform of the rat mu opioid receptor RT (rMOR1B) which differs in agonist induced desensitization from RT rMOR1."; RL FEBS Lett. 359:142-146(1995). CC -!- FUNCTION: Inhibits neurotransmitter release by reducing calcium CC ion currents and increasing potassium ion conductance. Receptor CC for beta-endorphin. CC -!- SUBUNIT: Forms a complex with G(alpha)z/i2 subunits and the RGSZ CC proteins, RGSZ17 and RGSZ20. The formation of this complex results CC in mu-opioid receptor desensitization. Interacts with RGSZ17 and CC RGSZ20 (By similarity). CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=8; CC Name=1; CC IsoId=P33535-1; Sequence=Displayed; CC Name=2; Synonyms=MOR1A; CC IsoId=P33535-2; Sequence=VSP_041828; CC Name=3; Synonyms=MOR1R; CC IsoId=P33535-3; Sequence=VSP_041829; CC Name=4; Synonyms=MOR1B; CC IsoId=P33535-4; Sequence=VSP_041830; CC Name=5; Synonyms=MOR1B2; CC IsoId=P33535-5; Sequence=VSP_041831; CC Name=6; Synonyms=MOR1C1; CC IsoId=P33535-6; Sequence=VSP_041832; CC Name=7; Synonyms=MOR-1C2; CC IsoId=P33535-7; Sequence=VSP_041833; CC Name=8; Synonyms=rMOR-1D; CC IsoId=P33535-8; Sequence=VSP_041834; CC -!- TISSUE SPECIFICITY: Brain. Is expressed in the cerebral cortex, CC caudate putamen, nucleus accumbens, septal nuclei, thalamus, CC hippocampus, and habenula. Not detected in cerebellum. CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. CC -!- SEQUENCE CAUTION: CC Sequence=AAQ77387.1; Type=Frameshift; Positions=Several; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D16349; BAA03852.1; -; mRNA. DR EMBL; L20684; AAA41643.1; -; mRNA. DR EMBL; L13069; AAA41630.1; -; mRNA. DR EMBL; U02083; AAA70049.1; -; mRNA. DR EMBL; L22455; AAA16075.1; -; mRNA. DR EMBL; U35424; AAA79180.1; -; mRNA. DR EMBL; AY309003; AAQ77387.1; ALT_FRAME; mRNA. DR EMBL; AY309004; AAQ77388.1; -; mRNA. DR EMBL; AY225402; AAP44725.1; -; mRNA. DR EMBL; AY225403; AAP44726.1; -; mRNA. DR EMBL; AY309000; AAQ77384.1; -; mRNA. DR EMBL; AY309002; AAQ77386.1; -; mRNA. DR EMBL; S77863; -; NOT_ANNOTATED_CDS; mRNA. DR EMBL; S75669; AAB33530.2; -; mRNA. DR IPI; IPI00655338; -. DR PIR; I56504; I56504. DR PIR; I56517; I56517. DR PIR; S69010; S69010. DR RefSeq; NP_001033686.1; NM_001038597.2. DR RefSeq; NP_001033688.2; NM_001038599.2. DR RefSeq; NP_001033689.1; NM_001038600.2. DR RefSeq; NP_001033690.1; NM_001038601.2. DR RefSeq; NP_037203.1; NM_013071.2. DR UniGene; Rn.10118; -. DR ProteinModelPortal; P33535; -. DR STRING; P33535; -. DR PhosphoSite; P33535; -. DR Ensembl; ENSRNOT00000044912; ENSRNOP00000043569; ENSRNOG00000018191. DR Ensembl; ENSRNOT00000051837; ENSRNOP00000051290; ENSRNOG00000018191. DR Ensembl; ENSRNOT00000060637; ENSRNOP00000057371; ENSRNOG00000018191. DR Ensembl; ENSRNOT00000064376; ENSRNOP00000063119; ENSRNOG00000018191. DR Ensembl; ENSRNOT00000066989; ENSRNOP00000061636; ENSRNOG00000018191. DR GeneID; 25601; -. DR KEGG; rno:25601; -. DR UCSC; NM_013071; rat. DR CTD; 4988; -. DR RGD; 3234; Oprm1. DR eggNOG; roNOG08631; -. DR GeneTree; ENSGT00600000084010; -. DR HOVERGEN; HBG106919; -. DR OrthoDB; EOG4KD6MF; -. DR NextBio; 607309; -. DR ArrayExpress; P33535; -. DR Genevestigator; P33535; -. DR GermOnline; ENSRNOG00000018191; Rattus norvegicus. DR GO; GO:0032839; C:dendrite cytoplasm; IDA:RGD. DR GO; GO:0032590; C:dendrite membrane; IDA:RGD. DR GO; GO:0005925; C:focal adhesion; IDA:RGD. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0005887; C:integral to plasma membrane; TAS:RGD. DR GO; GO:0005624; C:membrane fraction; IDA:RGD. DR GO; GO:0045121; C:membrane raft; IDA:RGD. DR GO; GO:0043204; C:perikaryon; IDA:RGD. DR GO; GO:0004988; F:mu-opioid receptor activity; IDA:RGD. DR GO; GO:0005515; F:protein binding; IPI:RGD. DR GO; GO:0002438; P:acute inflammatory response to antigenic stimulus; IEP:RGD. DR GO; GO:0031635; P:inhibition of adenylate cyclase activity by opioid receptor signaling pathway; IDA:RGD. DR GO; GO:0032100; P:positive regulation of appetite; IMP:RGD. DR GO; GO:0060079; P:regulation of excitatory postsynaptic membrane potential; IDA:RGD. DR GO; GO:0051930; P:regulation of sensory perception of pain; IDA:RGD. DR GO; GO:0042220; P:response to cocaine; IEP:RGD. DR GO; GO:0032094; P:response to food; IEP:RGD. DR GO; GO:0070848; P:response to growth factor stimulus; IEP:RGD. DR GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD. DR GO; GO:0043278; P:response to morphine; IEP:RGD. DR GO; GO:0042060; P:wound healing; IEP:RGD. DR InterPro; IPR000276; 7TM_GPCR_Rhodpsn. DR InterPro; IPR017452; GPCR_Rhodpsn_supfam. DR InterPro; IPR000105; Mu_opioid_rcpt. DR InterPro; IPR001418; Opioid_rcpt. DR KO; K04215; -. DR Pfam; PF00001; 7tm_1; 1. DR PRINTS; PR00237; GPCRRHODOPSN. DR PRINTS; PR00537; MUOPIOIDR. DR PRINTS; PR00384; OPIOIDR. DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1. DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1. PE 2: Evidence at transcript level; KW Alternative splicing; Cell membrane; Complete proteome; KW Disulfide bond; G-protein coupled receptor; Glycoprotein; Lipoprotein; KW Membrane; Palmitate; Phosphoprotein; Receptor; Reference proteome; KW Transducer; Transmembrane; Transmembrane helix. FT CHAIN 1 398 Mu-type opioid receptor. FT /FTId=PRO_0000069978. FT TOPO_DOM 1 64 Extracellular (Potential). FT TRANSMEM 65 94 Helical; Name=1; (Potential). FT TOPO_DOM 95 103 Cytoplasmic (Potential). FT TRANSMEM 104 121 Helical; Name=2; (Potential). FT TOPO_DOM 122 143 Extracellular (Potential). FT TRANSMEM 144 163 Helical; Name=3; (Potential). FT TOPO_DOM 164 193 Cytoplasmic (Potential). FT TRANSMEM 194 209 Helical; Name=4; (Potential). FT TOPO_DOM 210 234 Extracellular (Potential). FT TRANSMEM 235 257 Helical; Name=5; (Potential). FT TOPO_DOM 258 280 Cytoplasmic (Potential). FT TRANSMEM 281 303 Helical; Name=6; (Potential). FT TOPO_DOM 304 311 Extracellular (Potential). FT TRANSMEM 312 328 Helical; Name=7; (Potential). FT TOPO_DOM 329 398 Cytoplasmic (Potential). FT LIPID 351 351 S-palmitoyl cysteine (Potential). FT CARBOHYD 9 9 N-linked (GlcNAc...) (Potential). FT CARBOHYD 31 31 N-linked (GlcNAc...) (Potential). FT CARBOHYD 38 38 N-linked (GlcNAc...) (Potential). FT CARBOHYD 46 46 N-linked (GlcNAc...) (Potential). FT CARBOHYD 53 53 N-linked (GlcNAc...) (Potential). FT DISULFID 140 217 By similarity. FT VAR_SEQ 387 398 LENLEAETAPLP -> VCAF (in isoform 2). FT /FTId=VSP_041828. FT VAR_SEQ 387 398 LENLEAETAPLP -> GAEL (in isoform 3). FT /FTId=VSP_041829. FT VAR_SEQ 387 398 LENLEAETAPLP -> KIVLF (in isoform 4). FT /FTId=VSP_041830. FT VAR_SEQ 387 398 LENLEAETAPLP -> EPQSVET (in isoform 5). FT /FTId=VSP_041831. FT VAR_SEQ 387 398 LENLEAETAPLP -> PALAVSVAQIFTGYPSPTHGEKPCK FT SYRDRPRPCGRTWSLKSRAESNVEHFHCGAALIYNNVNFI FT (in isoform 6). FT /FTId=VSP_041832. FT VAR_SEQ 387 398 LENLEAETAPLP -> PALAVSVAQIFTGYPSPTHGEKPCK FT SYRDRPRPCGRTWSLKSRAESNVEHFHCGAALIYNNELKIG FT PVSWLQMPAHVLVRPW (in isoform 7). FT /FTId=VSP_041833. FT VAR_SEQ 387 398 LENLEAETAPLP -> T (in isoform 8). FT /FTId=VSP_041834. FT CONFLICT 237 237 F -> G (in Ref. 6; AAA79180). FT CONFLICT 238 238 I -> V (in Ref. 7; AAQ77386). FT CONFLICT 245 245 V -> I (in Ref. 3; AAA41630, 4; AAA70049 FT and 10; S77863). SQ SEQUENCE 398 AA; 44494 MW; 9C916DE7C1C33743 CRC64; MDSSTGPGNT SDCSDPLAQA SCSPAPGSWL NLSHVDGNQS DPCGLNRTGL GGNDSLCPQT GSPSMVTAIT IMALYSIVCV VGLFGNFLVM YVIVRYTKMK TATNIYIFNL ALADALATST LPFQSVNYLM GTWPFGTILC KIVISIDYYN MFTSIFTLCT MSVDRYIAVC HPVKALDFRT PRNAKIVNVC NWILSSAIGL PVMFMATTKY RQGSIDCTLT FSHPTWYWEN LLKICVFIFA FIMPVLIITV CYGLMILRLK SVRMLSGSKE KDRNLRRITR MVLVVVAVFI VCWTPIHIYV IIKALITIPE TTFQTVSWHF CIALGYTNSC LNPVLYAFLD ENFKRCFREF CIPTSSTIEQ QNSTRVRQNT REHPSTANTV DRTNHQLENL EAETAPLP //