ID PRP19_YEAST STANDARD; PRT; 503 AA. AC P32523; Q07870; DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1996, sequence version 2. DT 07-MAR-2006, entry version 47. DE Pre-mRNA-splicing factor PRP19. GN Name=PRP19; Synonyms=PSO4; OrderedLocusNames=YLL036C; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=93180836; PubMed=8441419; RA Cheng S.C., Tarn W.Y., Tsao T.Y., Abelson J.; RT "PRP19: a novel spliceosomal component."; RL Mol. Cell. Biol. 13:1876-1882(1993). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=97078680; PubMed=8918805; DOI=10.1093/nar/24.20.4009; RA Grey M., Duesterhoeft A., Henriques J.A.P., Brendel M.; RT "Allelism of PSO4 and PRP19 links pre-mRNA processing with RT recombination and error-prone DNA repair in Saccharomyces RT cerevisiae."; RL Nucleic Acids Res. 24:4009-4014(1996). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=S288c / AB972; RX MEDLINE=97313267; PubMed=9169871; RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., RA Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., RA Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., RA Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., RA Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., RA Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., RA Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., RA Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., RA Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., RA Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., RA Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., RA Hani J., Hoheisel J.D.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."; RL Nature 387:87-90(1997). RN [4] RP CHARACTERIZATION. RX MEDLINE=93180837; PubMed=7680101; RA Tarn W.Y., Lee K.R., Cheng S.C.; RT "The yeast PRP19 protein is not tightly associated with small nuclear RT RNAs, but appears to associate with the spliceosome after binding of RT U2 to the pre-mRNA and prior to formation of the functional RT spliceosome."; RL Mol. Cell. Biol. 13:1883-1891(1993). RN [5] RP LEVEL OF PROTEIN EXPRESSION. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [6] RP STRUCTURE BY NMR OF 1-56. RX PubMed=12627222; DOI=10.1038/nsb906; RA Ohi M.D., Vander Kooi C.W., Rosenberg J.A., Chazin W.J., Gould K.L.; RT "Structural insights into the U-box, a domain associated with multi- RT ubiquitination."; RL Nat. Struct. Biol. 10:250-255(2003). CC -!- FUNCTION: Not known. Appears to associate with the spliceosome CC after binding of U2 to the pre-mRNA and prior to formation of the CC functional spliceosome. Involved in DNA repair. CC -!- INTERACTION: CC Q03654:CEF1; NbExp=1; IntAct=EBI-493, EBI-476; CC Q12309:CLF1; NbExp=1; IntAct=EBI-493, EBI-484; CC P21374:ISY1; NbExp=1; IntAct=EBI-493, EBI-9382; CC P38302:NTC20; NbExp=1; IntAct=EBI-493, EBI-20921; CC Q06091:SNT309; NbExp=1; IntAct=EBI-493, EBI-818; CC Q04048:SYF1; NbExp=1; IntAct=EBI-493, EBI-540; CC P53277:SYF2; NbExp=1; IntAct=EBI-493, EBI-23308; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- MISCELLANEOUS: Present with 11700 molecules/cell. CC -!- SIMILARITY: Contains 1 U-box domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; L09721; AAA34912.1; -; Genomic_DNA. DR EMBL; X99770; CAA68103.1; -; Genomic_DNA. DR EMBL; Z73142; CAA97487.1; -; Genomic_DNA. DR PIR; S64787; S64787. DR PDB; 1N87; NMR; A=1-56. DR PDB; 2BAY; X-ray; A/B/C/D/E/F=1-58. DR IntAct; P32523; -. DR GermOnline; 142031; -. DR Ensembl; YLL036C; Saccharomyces cerevisiae. DR GenomeReviews; Y13138_GR; YLL036C. DR SGD; S000003959; PRP19. DR BioCyc; SCER-S28-01:SCER-S28-01-003848-MONOMER; -. DR LinkHub; P32523; -. DR GO; GO:0005739; C:mitochondrion; IDA. DR GO; GO:0005681; C:spliceosome complex; IDA. DR GO; GO:0005515; F:protein binding; IPI. DR GO; GO:0031202; F:RNA splicing factor activity, transesterifi...; IMP. DR GO; GO:0000398; P:nuclear mRNA splicing, via spliceosome; IDA. DR GO; GO:0006511; P:ubiquitin-dependent protein catabolism; IPI. DR InterPro; IPR003613; Ubox. DR SMART; SM00504; Ubox; 1. KW 3D-structure; Complete proteome; DNA damage; DNA repair; KW mRNA processing; mRNA splicing; Nuclear protein; Spliceosome; KW Ubl conjugation pathway. FT CHAIN 1 503 Pre-mRNA-splicing factor PRP19. FT /FTId=PRO_0000058587. FT DOMAIN 1 64 U-box. FT CONFLICT 239 239 Missing (in Ref. 1). FT STRAND 4 6 FT STRAND 11 15 FT TURN 17 19 FT STRAND 21 21 FT STRAND 23 24 FT HELIX 25 34 FT TURN 35 35 FT TURN 39 41 FT STRAND 44 44 FT HELIX 47 49 FT STRAND 51 52 SQ SEQUENCE 503 AA; 56570 MW; A44D8C7DD6F64185 CRC64; MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI VEIVPSAQQA SLTESTNSAT LKANYSIPNL LTSLQNEWDA IMLENFKLRS TLDSLTKKLS TVMYERDAAK LVAAQLLMEK NEDSKDLPKS SQQAVAITRE EFLQGLLQSS RDFVARGKLK APKWPILKNL ELLQAQNYSR NIKTFPYKEL NKSMYYDKWV CMCRCEDGAL HFTQLKDSKT ITTITTPNPR TGGEHPAIIS RGPCNRLLLL YPGNQITILD SKTNKVLREI EVDSANEIIY MYGHNEVNTE YFIWADNRGT IGFQSYEDDS QYIVHSAKSD VEYSSGVLHK DSLLLALYSP DGILDVYNLS SPDQASSRFP VDEEAKIKEV KFADNGYWMV VECDQTVVCF DLRKDVGTLA YPTYTIPEFK TGTVTYDIDD SGKNMIAYSN ESNSLTIYKF DKKTKNWTKD EESALCLQSD TADFTDMDVV CGDGGIAAIL KTNDSFNIVA LTP //