ID ROH3_HUMAN STANDARD; PRT; 346 AA. AC P31942; Q9BSX1; Q9NP53; Q9NP96; Q9NPA7; Q9NPI4; Q9UFU4; Q9Y4J5; DT 01-JUL-1993 (Rel. 26, Created) DT 28-FEB-2003 (Rel. 41, Last sequence update) DT 25-JAN-2005 (Rel. 46, Last annotation update) DE Heterogeneous nuclear ribonucleoprotein H3 (hnRNP H3) (hnRNP 2H9). GN Name=HNRPH3; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE (ISOFORM 1), AND SUBCELLULAR LOCATION. RX MEDLINE=97153147; PubMed=8999868; DOI=10.1074/jbc.272.3.1827; RA Mahe D., Mahl P., Gattoni R., Fischer N., Mattei M.-G., Stevenin J., RA Fuchs J.-P.; RT "Cloning of human 2H9 heterogeneous nuclear ribonucleoproteins. RT Relation with splicing and early heat shock-induced splicing arrest."; RL J. Biol. Chem. 272:1827-1836(1997). RN [2] RP NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2; 3; 4; 5 AND 6). RX MEDLINE=20461765; PubMed=10858537; RA Honore B.; RT "The hnRNP 2H9 gene, which is involved in the splicing reaction, is a RT multiply spliced gene."; RL Biochim. Biophys. Acta 1492:108-119(2000). RN [3] RP NUCLEOTIDE SEQUENCE (ISOFORM 4). RC TISSUE=Uterus; RG The German cDNA consortium; RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE (ISOFORM 2). RC TISSUE=Ovary, and Skin; RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899; RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E., RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human RT and mouse cDNA sequences."; RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). RN [5] RP PROTEIN SEQUENCE OF 94-104 AND 288-298. RC TISSUE=Keratinocytes; RX MEDLINE=93162043; PubMed=1286667; RA Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., RA Vandekerckhove J.; RT "Microsequences of 145 proteins recorded in the two-dimensional gel RT protein database of normal human epidermal keratinocytes."; RL Electrophoresis 13:960-969(1992). RN [6] RP PROTEIN SEQUENCE OF 116-127, AND METHYLATION SITE ARG-121. RX MEDLINE=21025454; PubMed=11152131; RA Yague J., Vazquez J., Lopez de Castro J.A.; RT "A post-translational modification of nuclear proteins, N(G),N(G)- RT dimethyl-Arg, found in a natural HLA class I peptide ligand."; RL Protein Sci. 9:2210-2217(2000). CC -!- FUNCTION: Involved in the splicing process and participates in CC early heat shock-induced splicing arrest. Due to their great CC structural variations the different isoforms may possess different CC functions in the splicing reaction. CC -!- SUBCELLULAR LOCATION: Nuclear. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=6; CC Name=1; CC IsoId=P31942-1; Sequence=Displayed; CC Name=2; Synonyms=2H9A; CC IsoId=P31942-2; Sequence=VSP_005840; CC Name=3; Synonyms=2H9B; CC IsoId=P31942-3; Sequence=VSP_005838; CC Name=4; Synonyms=2H9C; CC IsoId=P31942-4; Sequence=VSP_005839; CC Name=5; Synonyms=2H9D; CC IsoId=P31942-5; Sequence=VSP_005839, VSP_005843, VSP_005844; CC Name=6; Synonyms=2H9E; CC IsoId=P31942-6; Sequence=VSP_005841, VSP_005842, VSP_005843, CC VSP_005844; CC -!- SIMILARITY: Contains 2 RNA recognition motif (RRM) domains. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; L32610; AAD45179.1; -. DR EMBL; AF132360; AAF68843.1; -. DR EMBL; AF132360; AAF68844.1; -. DR EMBL; AF132360; AAF68845.1; -. DR EMBL; AF132360; AAF68846.1; -. DR EMBL; AF132360; AAF68847.1; -. DR EMBL; AF132360; AAF68848.1; -. DR EMBL; AF132361; AAF68849.1; -. DR EMBL; AF132362; AAF68850.1; -. DR EMBL; AF132363; AAF68851.1; -. DR EMBL; AF132364; AAF68852.1; -. DR EMBL; AL117395; CAB55897.1; -. DR EMBL; BC004511; AAH04511.2; -. DR EMBL; BC039824; AAH39824.1; -. DR PIR; T17207; T17207. DR Aarhus/Ghent-2DPAGE; 2222; IEF. DR Ensembl; ENSG00000096746; Homo sapiens. DR Genew; HGNC:5043; HNRPH3. DR MIM; 602324; -. DR InterPro; IPR000504; RNA_rec_mot. DR Pfam; PF00076; RRM_1; 2. DR PROSITE; PS50102; RRM; 2. KW Alternative splicing; Direct protein sequencing; Methylation; KW mRNA processing; Nuclear protein; Polymorphism; Repeat; KW Ribonucleoprotein; RNA-binding. FT DOMAIN 16 93 RNA-binding (RRM) 1. FT DOMAIN 108 344 Gly-rich. FT DOMAIN 195 270 RNA-binding (RRM) 2. FT MOD_RES 121 121 Asymmetric dimethylarginine. FT VARSPLIC 1 49 Missing (in isoform 3). FT /FTId=VSP_005838. FT VARSPLIC 1 131 Missing (in isoform 4 and isoform 5). FT /FTId=VSP_005839. FT VARSPLIC 132 146 Missing (in isoform 2). FT /FTId=VSP_005840. FT VARSPLIC 1 137 Missing (in isoform 6). FT /FTId=VSP_005841. FT VARSPLIC 138 145 RGGDGYDG -> MCFSLNYT (in isoform 6). FT /FTId=VSP_005842. FT VARSPLIC 259 276 QHRYIELFLNSTPGGGSG -> RKWCLWHTILFPKREFIK FT (in isoform 5 and isoform 6). FT /FTId=VSP_005843. FT VARSPLIC 277 346 Missing (in isoform 5 and isoform 6). FT /FTId=VSP_005844. FT VARIANT 163 163 N -> S (in dbSNP:2273903). FT /FTId=VAR_020333. SQ SEQUENCE 346 AA; 36926 MW; F7D14C2947930E9E CRC64; MDWVMKHNGP NDASDGTVRL RGLPFGCSKE EIVQFFQGLE IVPNGITLTM DYQGRSTGEA FVQFASKEIA ENALGKHKER IGHRYIEIFR SSRSEIKGFY DPPRRLLGQR PGPYDRPIGG RGGYYGAGRG SMYDRMRRGG DGYDGGYGGF DDYGGYNNYG YGNDGFDDRM RDGRGMGGHG YGGAGDASSG FHGGHFVHMR GLPFRATEND IANFFSPLNP IRVHIDIGAD GRATGEADVE FVTHEDAVAA MSKDKNNMQH RYIELFLNST PGGGSGMGGS GMGGYGRDGM DNQGGYGSVG RMGMGNNYSG GYGTPDGLGG YGRGGGGSGG YYGQGGMSGG GWRGMY //