ID EAA2_RAT Reviewed; 573 AA. AC P31596; DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1993, sequence version 2. DT 02-JUN-2021, entry version 163. DE RecName: Full=Excitatory amino acid transporter 2; DE AltName: Full=GLT-1 {ECO:0000303|PubMed:11860269, ECO:0000303|PubMed:1448170}; DE AltName: Full=Sodium-dependent glutamate/aspartate transporter 2; DE Short=GLUT-R; DE AltName: Full=Solute carrier family 1 member 2; GN Name=Slc1a2; Synonyms=Eaat2, Glt1; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Rattus. OX NCBI_TaxID=10116; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE RP SPECIFICITY. RC TISSUE=Brain; RX PubMed=1448170; DOI=10.1038/360464a0; RA Pines G., Danbolt N.C., Bjoeraas M., Zhang Y., Bendahan A., Eide L., RA Koepsell H., Storm-Mathisen J., Seeberg E., Kanner B.I.; RT "Cloning and expression of a rat brain L-glutamate transporter."; RL Nature 360:464-467(1992). RN [2] RP ERRATUM OF PUBMED:1448170. RA Pines G., Danbolt N.C., Bjoeraas M., Zhang Y., Bendahan A., Eide L., RA Koepsell H., Storm-Mathisen J., Seeberg E., Kanner B.I.; RL Nature 360:768-768(1992). RN [3] RP SEQUENCE REVISION TO 260-289. RX PubMed=8099882; DOI=10.1016/0014-5793(93)81421-u; RA Kanner B.I.; RT "Glutamate transporters from brain. A novel neurotransmitter transporter RT family."; RL FEBS Lett. 325:95-99(1993). RN [4] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Forebrain; RA Roginski R.S., Choudhury K., Meiners S., Marone M., Basma A.N., RA Geller H.M.; RL Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GLT-1A). RC TISSUE=Hepatoma; RX PubMed=11068035; DOI=10.1016/s0014-5793(00)02114-1; RA Pollard M., McGivan J.; RT "The rat hepatoma cell line H4-II-E-C3 expresses high activities of the RT high-affinity glutamate transporter GLT-1A."; RL FEBS Lett. 484:74-76(2000). RN [6] RP PROTEIN SEQUENCE OF 158-173, AND IDENTIFICATION BY MASS SPECTROMETRY. RC STRAIN=Sprague-Dawley; TISSUE=Brain; RA Lubec G., Kang S.U.; RL Submitted (JUL-2007) to UniProtKB. RN [7] RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF LYS-298 AND HIS-326. RX PubMed=7913472; RA Zhang Y., Pines G., Kanner B.I.; RT "Histidine 326 is critical for the function of GLT-1, a (Na+ + K+)-coupled RT glutamate transporter from rat brain."; RL J. Biol. Chem. 269:19573-19577(1994). RN [8] RP INTERACTION WITH AJUBA. RX PubMed=11860269; DOI=10.1006/mcne.2001.1066; RA Marie H., Billups D., Bedford F.K., Dumoulin A., Goyal R.K., Longmore G.D., RA Moss S.J., Attwell D.; RT "The amino terminus of the glial glutamate transporter GLT-1 interacts with RT the LIM protein Ajuba."; RL Mol. Cell. Neurosci. 19:152-164(2002). RN [9] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3; SER-21; SER-24; SER-25 AND RP SER-505, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=22673903; DOI=10.1038/ncomms1871; RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., RA Olsen J.V.; RT "Quantitative maps of protein phosphorylation sites across 14 different rat RT organs and tissues."; RL Nat. Commun. 3:876-876(2012). CC -!- FUNCTION: Sodium-dependent, high-affinity amino acid transporter that CC mediates the uptake of L-glutamate and also L-aspartate and D-aspartate CC (PubMed:1448170, PubMed:7913472). Functions as a symporter that CC transports one amino acid molecule together with two or three Na(+) CC ions and one proton, in parallel with the counter-transport of one K(+) CC ion (PubMed:1448170). Mediates Cl(-) flux that is not coupled to amino CC acid transport; this avoids the accumulation of negative charges due to CC aspartate and Na(+) symport (By similarity). Essential for the rapid CC removal of released glutamate from the synaptic cleft, and for CC terminating the postsynaptic action of glutamate (By similarity). CC {ECO:0000250|UniProtKB:P43004, ECO:0000250|UniProtKB:P43006, CC ECO:0000269|PubMed:1448170, ECO:0000269|PubMed:7913472}. CC -!- SUBUNIT: Homotrimer (By similarity). Interacts with AJUBA CC (PubMed:11860269). {ECO:0000250|UniProtKB:P43004, CC ECO:0000269|PubMed:11860269}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1448170, CC ECO:0000269|PubMed:7913472}; Multi-pass membrane protein CC {ECO:0000250|UniProtKB:P43004}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=Glt1; CC IsoId=P31596-1; Sequence=Displayed; CC Name=Glt-1A; CC IsoId=P31596-2; Sequence=VSP_006266; CC -!- TISSUE SPECIFICITY: Localized in brain and is highly enriched in the CC Purkinje cell layer in cerebellum. {ECO:0000269|PubMed:1448170}. CC -!- DOMAIN: Contains eight transmembrane regions plus two helical hairpins CC that dip into the membrane. These helical hairpin structures play an CC important role in the transport process. The first enters the membrane CC from the cytoplasmic side, the second one from the extracellular side. CC During the transport cycle, the regions involved in amino acid CC transport, and especially the helical hairpins, move vertically by CC about 15-18 Angstroms, alternating between exposure to the aqueous CC phase and reinsertion in the lipid bilayer. In contrast, the regions CC involved in trimerization do not move. {ECO:0000250|UniProtKB:P43003}. CC -!- PTM: Glycosylated. {ECO:0000250|UniProtKB:P43006}. CC -!- PTM: Palmitoylation at Cys-38 is not required for correct subcellular CC localization, but is important for glutamate uptake activity. CC {ECO:0000250|UniProtKB:P43006}. CC -!- SIMILARITY: Belongs to the dicarboxylate/amino acid:cation symporter CC (DAACS) (TC 2.A.23) family. SLC1A2 subfamily. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAA93062.1; Type=Erroneous initiation; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X67857; CAA48042.1; ALT_SEQ; mRNA. DR EMBL; U15098; AAA93061.1; -; mRNA. DR EMBL; U15098; AAA93062.1; ALT_INIT; mRNA. DR EMBL; AF297648; AAG13411.1; -; mRNA. DR PIR; S28901; S28901. DR RefSeq; NP_001289018.1; NM_001302089.1. DR RefSeq; NP_058911.2; NM_017215.2. DR BioGRID; 248124; 3. DR CORUM; P31596; -. DR IntAct; P31596; 3. DR STRING; 10116.ENSRNOP00000007604; -. DR ChEMBL; CHEMBL2900; -. DR GuidetoPHARMACOLOGY; 869; -. DR TCDB; 2.A.23.2.2; the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family. DR CarbonylDB; P31596; -. DR GlyGen; P31596; 2 sites, 8 N-linked glycans (1 site). DR iPTMnet; P31596; -. DR PhosphoSitePlus; P31596; -. DR SwissPalm; P31596; -. DR PaxDb; P31596; -. DR PRIDE; P31596; -. DR GeneID; 29482; -. DR KEGG; rno:29482; -. DR UCSC; RGD:3697; rat. [P31596-1] DR CTD; 6506; -. DR RGD; 3697; Slc1a2. DR eggNOG; KOG3787; Eukaryota. DR InParanoid; P31596; -. DR OMA; WILTITP; -. DR OrthoDB; 1184392at2759; -. DR PhylomeDB; P31596; -. DR TreeFam; TF315206; -. DR Reactome; R-RNO-210455; Astrocytic Glutamate-Glutamine Uptake And Metabolism. DR Reactome; R-RNO-210500; Glutamate Neurotransmitter Release Cycle. DR Reactome; R-RNO-425393; Transport of inorganic cations/anions and amino acids/oligopeptides. DR PRO; PR:P31596; -. DR Proteomes; UP000002494; Unplaced. DR GO; GO:0097449; C:astrocyte projection; IDA:ARUK-UCL. DR GO; GO:0030673; C:axolemma; ISO:RGD. DR GO; GO:0030424; C:axon; ISO:RGD. DR GO; GO:0044297; C:cell body; IDA:ARUK-UCL. DR GO; GO:0009986; C:cell surface; ISO:RGD. DR GO; GO:0043198; C:dendritic shaft; IDA:RGD. DR GO; GO:0043197; C:dendritic spine; IDA:RGD. DR GO; GO:0098978; C:glutamatergic synapse; ISO:RGD. DR GO; GO:0005887; C:integral component of plasma membrane; IMP:UniProtKB. DR GO; GO:0099056; C:integral component of presynaptic membrane; ISO:RGD. DR GO; GO:0016020; C:membrane; ISO:RGD. DR GO; GO:0098796; C:membrane protein complex; IDA:ARUK-UCL. DR GO; GO:0045121; C:membrane raft; ISO:RGD. DR GO; GO:0043005; C:neuron projection; IDA:RGD. DR GO; GO:0044306; C:neuron projection terminus; ISO:RGD. DR GO; GO:0005886; C:plasma membrane; IDA:RGD. DR GO; GO:0042734; C:presynaptic membrane; IDA:RGD. DR GO; GO:0045202; C:synapse; IDA:RGD. DR GO; GO:0031982; C:vesicle; ISO:RGD. DR GO; GO:0008509; F:anion transmembrane transporter activity; ISO:RGD. DR GO; GO:0033229; F:cysteine transmembrane transporter activity; ISO:RGD. DR GO; GO:0015501; F:glutamate:sodium symporter activity; IDA:UniProtKB. DR GO; GO:0005314; F:high-affinity glutamate transmembrane transporter activity; IDA:UniProtKB. DR GO; GO:0005313; F:L-glutamate transmembrane transporter activity; ISO:RGD. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0030534; P:adult behavior; ISO:RGD. DR GO; GO:0098656; P:anion transmembrane transport; ISO:RGD. DR GO; GO:0071314; P:cellular response to cocaine; ISO:RGD. DR GO; GO:0031668; P:cellular response to extracellular stimulus; ISO:RGD. DR GO; GO:0070779; P:D-aspartate import across plasma membrane; ISO:RGD. DR GO; GO:0006750; P:glutathione biosynthetic process; ISO:RGD. DR GO; GO:0098712; P:L-glutamate import across plasma membrane; ISS:UniProtKB. DR GO; GO:0015813; P:L-glutamate transmembrane transport; IMP:RGD. DR GO; GO:0010259; P:multicellular organism aging; ISO:RGD. DR GO; GO:0035264; P:multicellular organism growth; ISO:RGD. DR GO; GO:0007399; P:nervous system development; ISO:RGD. DR GO; GO:0098810; P:neurotransmitter reuptake; ISO:RGD. DR GO; GO:0006836; P:neurotransmitter transport; TAS:RGD. DR GO; GO:0046326; P:positive regulation of glucose import; ISO:RGD. DR GO; GO:0070207; P:protein homotrimerization; ISS:UniProtKB. DR GO; GO:0043200; P:response to amino acid; ISO:RGD. DR GO; GO:0042493; P:response to drug; ISO:RGD. DR GO; GO:0009416; P:response to light stimulus; ISO:RGD. DR GO; GO:0009611; P:response to wounding; ISO:RGD. DR GO; GO:0021537; P:telencephalon development; ISO:RGD. DR GO; GO:0007632; P:visual behavior; ISO:RGD. DR Gene3D; 1.10.3860.10; -; 2. DR InterPro; IPR001991; Na-dicarboxylate_symporter. DR InterPro; IPR018107; Na-dicarboxylate_symporter_CS. DR InterPro; IPR036458; Na:dicarbo_symporter_sf. DR Pfam; PF00375; SDF; 1. DR SUPFAM; SSF118215; SSF118215; 1. DR PROSITE; PS00713; NA_DICARBOXYL_SYMP_1; 1. DR PROSITE; PS00714; NA_DICARBOXYL_SYMP_2; 1. PE 1: Evidence at protein level; KW Alternative splicing; Amino-acid transport; Cell membrane; Chloride; KW Direct protein sequencing; Glycoprotein; Lipoprotein; Membrane; KW Metal-binding; Palmitate; Phosphoprotein; Potassium; Reference proteome; KW Sodium; Symport; Transmembrane; Transmembrane helix; Transport. FT CHAIN 1..573 FT /note="Excitatory amino acid transporter 2" FT /id="PRO_0000202063" FT TOPO_DOM 1..44 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 45..64 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 88..108 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 121..142 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 235..258 FT /note="Helical; Name=4" FT /evidence="ECO:0000250|UniProtKB:P43003" FT TRANSMEM 268..295 FT /note="Helical; Name=5" FT /evidence="ECO:0000250|UniProtKB:P43003" FT TRANSMEM 317..338 FT /note="Helical; Name=6" FT /evidence="ECO:0000250|UniProtKB:P43003" FT INTRAMEM 344..374 FT /note="Discontinuously helical" FT /evidence="ECO:0000250|UniProtKB:P43003" FT TRANSMEM 384..410 FT /note="Helical; Name=7" FT /evidence="ECO:0000250|UniProtKB:P43003" FT INTRAMEM 424..457 FT /note="Discontinuously helical" FT /evidence="ECO:0000250|UniProtKB:P43003" FT TRANSMEM 471..492 FT /note="Helical; Name=8" FT /evidence="ECO:0000250|UniProtKB:P43003" FT REGION 1..29 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 361..363 FT /note="Aspartate binding" FT /evidence="ECO:0000250|UniProtKB:P43003" FT REGION 441..445 FT /note="Aspartate binding" FT /evidence="ECO:0000250|UniProtKB:P43003" FT COMPBIAS 15..29 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT METAL 392 FT /note="Sodium 1; via carbonyl oxygen" FT /evidence="ECO:0000250|UniProtKB:O59010" FT METAL 394 FT /note="Sodium 2; via carbonyl oxygen" FT /evidence="ECO:0000250|UniProtKB:P43003" FT METAL 396 FT /note="Sodium 1" FT /evidence="ECO:0000250|UniProtKB:O59010" FT METAL 481 FT /note="Sodium 1; via carbonyl oxygen" FT /evidence="ECO:0000250|UniProtKB:O59010" FT METAL 485 FT /note="Sodium 1" FT /evidence="ECO:0000250|UniProtKB:O59010" FT BINDING 400 FT /note="Aspartate" FT /evidence="ECO:0000250|UniProtKB:P43003" FT BINDING 474 FT /note="Aspartate" FT /evidence="ECO:0000250|UniProtKB:P43003" FT BINDING 481 FT /note="Aspartate" FT /evidence="ECO:0000250|UniProtKB:P43003" FT MOD_RES 3 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:22673903" FT MOD_RES 21 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:22673903" FT MOD_RES 24 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:22673903" FT MOD_RES 25 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:22673903" FT MOD_RES 505 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:22673903" FT MOD_RES 520 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:P43006" FT MOD_RES 531 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:P43006" FT MOD_RES 533 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:P43006" FT MOD_RES 538 FT /note="Phosphotyrosine" FT /evidence="ECO:0000250|UniProtKB:P43006" FT MOD_RES 543 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:P43006" FT MOD_RES 559 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:P43006" FT MOD_RES 563 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:P43006" FT LIPID 38 FT /note="S-palmitoyl cysteine" FT /evidence="ECO:0000250|UniProtKB:P43006" FT CARBOHYD 205 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 215 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT VAR_SEQ 1..6 FT /note="MASTEG -> MVS (in isoform Glt-1A)" FT /evidence="ECO:0000303|PubMed:11068035" FT /id="VSP_006266" FT MUTAGEN 298 FT /note="K->H,R: Normal transporter activity." FT /evidence="ECO:0000269|PubMed:7913472" FT MUTAGEN 298 FT /note="K->N,T: Reduced transporter activity." FT /evidence="ECO:0000269|PubMed:7913472" FT MUTAGEN 326 FT /note="H->N,T,K,R: No transporter activity." FT /evidence="ECO:0000269|PubMed:7913472" FT CONFLICT 521 FT /note="V -> I (in Ref. 4; AAA93061/AAA93062)" FT /evidence="ECO:0000305" SQ SEQUENCE 573 AA; 62106 MW; 8C51D30954E00E7F CRC64; MASTEGANNM PKQVEVRMHD SHLSSEEPKH RNLGMRMCDK LGKNLLLSLT VFGVILGAVC GGLLRLAAPI HPDVVMLIAF PGDILMRMLK MLILPLIISS LITGLSGLDA KASGRLGTRA MVYYMSTTII AAVLGVILVL AIHPGNPKLK KQLGPGKKND EVSSLDAFLD LIRNLFPENL VQACFQQIQT VTKKVLVAPP SEEANTTKAV ISLLNETMNE APEETKIVIK KGLEFKDGMN VLGLIGFFIA FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI FQAWITALGT ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT ALYEAVAAIF IAQMNGVILD GGQIVTVSLT ATLASIGAAS IPSAGLVTML LILTAVGLPT EDISLLVAVD WLLDRMRTSV NVVGDSFGAG IVYHLSKSEL DTIDSQHRMH EDIEMTKTQS VYDDTKNHRE SNSNQCVYAA HNSVVIDECK VTLAANGKSA DCSVEEEPWK REK //