ID EAT2_RAT STANDARD; PRT; 573 AA. AC P31596; DT 01-JUL-1993 (Rel. 26, Created) DT 01-OCT-1993 (Rel. 27, Last sequence update) DT 15-JUL-1998 (Rel. 36, Last annotation update) DE EXCITATORY AMINO ACID TRANSPORTER 2 (SODIUM-DEPENDENT DE GLUTAMATE/ASPARTATE TRANSPORTER 2) (GLUT-R) (GLT-1). GN SLC1A2 OR EAAT2 OR GLT1. OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Rattus. OX NCBI_TaxID=10116; RN [1] RP SEQUENCE FROM N.A. RC TISSUE=Brain; RX MEDLINE=93078876; PubMed=1448170; RA Pines G., Danbolt N.C., Bjoeraas M., Zhang Y., Bendahan A., Eide L., RA Koepsell H., Storm-Mathisen J., Seeberg E., Kanner B.I.; RT "Cloning and expression of a rat brain L-glutamate transporter."; RL Nature 360:464-467(1992). RN [2] RP ERRATUM. RA Pines G., Danbolt N.C., Bjoeraas M., Zhang Y., Bendahan A., Eide L., RA Koepsell H., Storm-Mathisen J., Seeberg E., Kanner B.I.; RL Nature 360:768-768(1992). RN [3] RP REVISIONS TO 260-289. RX MEDLINE=93292659; PubMed=8099882; RA Kanner B.I.; RT "Glutamate transporters from brain. A novel neurotransmitter RT transporter family."; RL FEBS Lett. 325:95-99(1993). RN [4] RP SEQUENCE FROM N.A. RC TISSUE=Forebrain; RA Roginski R.S., Choudhury K., Meiners S., Marone M., Basma A.N., RA Geller H.M.; RL Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases. RN [5] RP MUTAGENESIS OF LYS-298 AND HIS-326. RX MEDLINE=94308247; PubMed=7913472; RA Zhang Y., Pines G., Kanner B.I.; RT "Histidine 326 is critical for the function of GLT-1, a (Na+ + K+)- RT coupled glutamate transporter from rat brain."; RL J. Biol. Chem. 269:19573-19577(1994). CC -!- FUNCTION: TRANSPORTS L-GLUTAMATE AND ALSO L- AND D-ASPARTATE. CC ESSENTIAL FOR TERMINATING THE POSTSYNAPTIC ACTION OF GLUTAMATE BY CC RAPIDLY REMOVING RELEASED GLUTAMATE FROM THE SYNAPTIC CLEFT. ACTS CC AS A SYMPORT BY CO-TRANSPORTING SODIUM. CC -!- SUBCELLULAR LOCATION: INTEGRAL MEMBRANE PROTEIN. CC -!- TISSUE SPECIFICITY: LOCALIZED IN BRAIN AND IS HIGHLY ENRICHED IN CC THE PURKINJE CELL LAYER IN CEREBELLUM. CC -!- PTM: GLYCOSYLATED. CC -!- SIMILARITY: BELONGS TO THE SODIUM:DICARBOXYLATE SYMPORTER FAMILY CC (SDF). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X67857; CAA48042.1; ALT_SEQ. DR EMBL; U15098; AAA93061.1; -. DR EMBL; U15098; AAA93062.1; ALT_INIT. DR InterPro; IPR001991; -. DR Pfam; PF00375; SDF; 1. DR PRINTS; PR00173; EDTRNSPORT. DR PROSITE; PS00713; NA_DICARBOXYL_SYMP_1; 1. DR PROSITE; PS00714; NA_DICARBOXYL_SYMP_2; 1. KW Transport; Transmembrane; Glycoprotein; Symport; Multigene family. FT DOMAIN 1 44 CYTOPLASMIC (POTENTIAL). FT TRANSMEM 45 64 POTENTIAL. FT TRANSMEM 88 108 POTENTIAL. FT TRANSMEM 121 142 POTENTIAL. FT DOMAIN 143 238 EXTRACELLULAR (POTENTIAL). FT TRANSMEM 239 258 POTENTIAL. FT TRANSMEM 279 300 POTENTIAL. FT TRANSMEM 316 338 POTENTIAL. FT TRANSMEM 405 429 POTENTIAL. FT TRANSMEM 436 458 POTENTIAL. FT CARBOHYD 205 205 N-LINKED (GLCNAC...) (POTENTIAL). FT CARBOHYD 215 215 N-LINKED (GLCNAC...) (POTENTIAL). FT MUTAGEN 298 298 K->N,T: REDUCED TRANSPORTER ACTIVITY. FT MUTAGEN 298 298 K->H,R: NORMAL TRANSPORTER ACTIVITY. FT MUTAGEN 326 326 H->N,T,K,R: NO TRANSPORTER ACTIVITY. FT CONFLICT 521 521 V -> I (IN REF. 4). SQ SEQUENCE 573 AA; 62106 MW; 8C51D30954E00E7F CRC64; MASTEGANNM PKQVEVRMHD SHLSSEEPKH RNLGMRMCDK LGKNLLLSLT VFGVILGAVC GGLLRLAAPI HPDVVMLIAF PGDILMRMLK MLILPLIISS LITGLSGLDA KASGRLGTRA MVYYMSTTII AAVLGVILVL AIHPGNPKLK KQLGPGKKND EVSSLDAFLD LIRNLFPENL VQACFQQIQT VTKKVLVAPP SEEANTTKAV ISLLNETMNE APEETKIVIK KGLEFKDGMN VLGLIGFFIA FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI FQAWITALGT ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT ALYEAVAAIF IAQMNGVILD GGQIVTVSLT ATLASIGAAS IPSAGLVTML LILTAVGLPT EDISLLVAVD WLLDRMRTSV NVVGDSFGAG IVYHLSKSEL DTIDSQHRMH EDIEMTKTQS VYDDTKNHRE SNSNQCVYAA HNSVVIDECK VTLAANGKSA DCSVEEEPWK REK //