ID POLG_DEN27 Reviewed; 3391 AA. AC P29991; DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot. DT 01-APR-1993, sequence version 1. DT 03-APR-2007, entry version 63. DE Genome polyprotein [Contains: Capsid protein C (Core protein); DE Envelope protein M (Matrix protein); Major envelope protein E; Non- DE structural protein 1 (NS1); Non-structural protein 2A (NS2A); DE Flavivirin protease NS2B regulatory subunit; Flavivirin protease NS3 DE catalytic subunit (EC 3.4.21.91); Non-structural protein 4A (NS4A); DE Non-structural protein 4B (NS4B); RNA-directed RNA polymerase DE (EC 2.7.7.48) (NS5)]. OS Dengue virus type 2 (strain 16681-PDK53) (DENV-2). OC Viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; OC Flavivirus; Dengue virus group. OX NCBI_TaxID=31635; OH NCBI_TaxID=7159; Aedes aegypti (Yellowfever mosquito). OH NCBI_TaxID=299627; Aedes furcifer. OH NCBI_TaxID=299628; Aedes taylori. OH NCBI_TaxID=9538; Erythrocebus patas (Red guenon) (Cercopithecus patas). OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RX MEDLINE=92188532; PubMed=1312269; DOI=10.1016/0042-6822(92)90460-7; RA Blok J., McWilliam S.M., Butler H.C., Gibbs A.J., Weiller G., RA Herring B.L., Hemsley A.C., Aaskov J.G., Yoksan S., Bhamarapravati N.; RT "Comparison of a dengue-2 virus and its candidate vaccine derivative: RT sequence relationships with the flaviviruses and other viruses."; RL Virology 187:573-590(1992). CC -!- FUNCTION: The small proteins NS2A, NS4A and NS4B are hydrophobic, CC suggesting a possible membrane-related function. NS5 may play a CC role in the viral RNA replication. The NS2B/NS3 protease complex CC processes the viral polyprotein. CC -!- CATALYTIC ACTIVITY: Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds CC in which each of the Xaa can be either Arg or Lys and Yaa can be CC either Ser or Ala. CC -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate CC + RNA(n+1). CC -!- SUBUNIT: NS3 and NS2B form a heterodimer. NS3 is the catalytic CC subunit, whereas NS2B strongly stimulates the latter (By CC similarity). CC -!- PTM: Specific enzymatic cleavages in vivo yield mature proteins CC (By similarity). CC -!- MISCELLANEOUS: The virion of this virus is a nucleocapsid covered CC by a lipoprotein envelope. The envelope contains two proteins: the CC protein M and glycoprotein E. The nucleocapsid is a complex of CC protein C and mRNA. In immature particles, there are 60 CC icosaedrally organized trimeric spikes on the surface. Each spike CC consists of three heterodimers of envelope protein M precursor CC (prM) and envelope protein E (By similarity). CC -!- SIMILARITY: Contains 1 helicase ATP-binding domain. CC -!- SIMILARITY: Contains 1 peptidase S7 domain. CC -!- SIMILARITY: Contains 1 RdRp catalytic domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M84728; AAA73186.1; -; Genomic_RNA. DR EMBL; M84727; -; NOT_ANNOTATED_CDS; Genomic_RNA. DR PIR; B42451; GNWV26. DR HSSP; Q88653; 1OKE. DR SMR; P29991; 21-100, 281-674, 1480-1656, 1646-2093, 2498-2759. DR InterPro; IPR014001; DEAD-like_N. DR InterPro; IPR002464; DEAH_box. DR InterPro; IPR013755; Flav_glyE_cen_1. DR InterPro; IPR013756; Flav_glyE_cen_2. DR InterPro; IPR011999; Flav_glyE_cen_dm. DR InterPro; IPR013754; Flav_glyE_dim. DR InterPro; IPR001122; Flavi_capsidC. DR InterPro; IPR011492; Flavi_DEAD. DR InterPro; IPR000069; Flavi_M. DR InterPro; IPR001157; Flavi_NS1. DR InterPro; IPR000752; Flavi_NS2A. DR InterPro; IPR000487; Flavi_NS2B. DR InterPro; IPR000404; Flavi_NS4A. DR InterPro; IPR001528; Flavi_NS4B. DR InterPro; IPR000208; Flavi_NS5. DR InterPro; IPR002535; Flavi_propep. DR InterPro; IPR000336; Flv_glyE_Ig-like. DR InterPro; IPR014021; Helic_SF1/SF2_ATP_bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR001850; Peptidase_S7. DR InterPro; IPR007094; RNA_pol_PSvir. DR InterPro; IPR002877; RrmJFtsJ_mtfrase. DR InterPro; IPR011998; Vrl_glyE_cen_dim. DR Gene3D; G3DSA:3.30.387.10; Flav_glyE_cen_1; 1. DR Gene3D; G3DSA:3.30.67.10; Flav_glyE_cen_2; 1. DR Gene3D; G3DSA:2.60.98.10; Flav_glyE_dim; 1. DR Gene3D; G3DSA:2.60.40.350; Flv_glyE_Ig-like; 1. DR Pfam; PF01003; Flavi_capsid; 1. DR Pfam; PF07652; Flavi_DEAD; 1. DR Pfam; PF02832; Flavi_glycop_C; 1. DR Pfam; PF00869; Flavi_glycoprot; 1. DR Pfam; PF01004; Flavi_M; 1. DR Pfam; PF00948; Flavi_NS1; 1. DR Pfam; PF01005; Flavi_NS2A; 1. DR Pfam; PF01002; Flavi_NS2B; 1. DR Pfam; PF01350; Flavi_NS4A; 1. DR Pfam; PF01349; Flavi_NS4B; 1. DR Pfam; PF00972; Flavi_NS5; 1. DR Pfam; PF01570; Flavi_propep; 1. DR Pfam; PF01728; FtsJ; 1. DR Pfam; PF00949; Peptidase_S7; 1. DR ProDom; PD001496; Flavi_NS1; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR PROSITE; PS00690; DEAH_ATP_HELICASE; FALSE_NEG. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. KW ATP-binding; Capsid protein; Cleavage on pair of basic residues; KW Core protein; Envelope protein; Glycoprotein; Helicase; Hydrolase; KW Membrane; Nucleotide-binding; Nucleotidyltransferase; RNA replication; KW RNA-directed RNA polymerase; Transferase; Transmembrane; KW Virion protein. FT CHAIN 1 114 Capsid protein C. FT /FTId=PRO_0000037936. FT PROPEP 115 205 FT /FTId=PRO_0000037937. FT CHAIN 206 280 Envelope protein M. FT /FTId=PRO_0000037938. FT CHAIN 281 775 Major envelope protein E. FT /FTId=PRO_0000037939. FT CHAIN 776 1127 Non-structural protein 1. FT /FTId=PRO_0000037940. FT CHAIN 1128 1345 Non-structural protein 2A. FT /FTId=PRO_0000037941. FT CHAIN 1346 1475 Flavivirin protease NS2B regulatory FT subunit. FT /FTId=PRO_0000037942. FT CHAIN 1476 2093 Flavivirin protease NS3 catalytic FT subunit. FT /FTId=PRO_0000037943. FT CHAIN 2094 2243 Non-structural protein 4A. FT /FTId=PRO_0000037944. FT CHAIN 2244 2491 Non-structural protein 4B. FT /FTId=PRO_0000037945. FT CHAIN 2492 3391 RNA-directed RNA polymerase. FT /FTId=PRO_0000037946. FT TRANSMEM 50 66 Potential. FT TRANSMEM 102 118 Potential. FT TRANSMEM 268 284 Potential. FT TRANSMEM 727 743 Potential. FT TRANSMEM 757 773 Potential. FT TRANSMEM 1158 1174 Potential. FT TRANSMEM 1272 1288 Potential. FT TRANSMEM 1294 1310 Potential. FT TRANSMEM 1351 1367 Potential. FT TRANSMEM 1373 1389 Potential. FT TRANSMEM 1448 1464 Potential. FT TRANSMEM 2148 2164 Potential. FT TRANSMEM 2174 2190 Potential. FT TRANSMEM 2197 2213 Potential. FT TRANSMEM 2227 2243 Potential. FT TRANSMEM 2352 2368 Potential. FT TRANSMEM 2411 2427 Potential. FT DOMAIN 1482 1654 Peptidase S7. FT DOMAIN 1655 1811 Helicase ATP-binding. FT DOMAIN 3019 3168 RdRp catalytic. FT NP_BIND 1668 1675 ATP (Potential). FT MOTIF 1759 1762 DEAH box. FT ACT_SITE 1526 1526 Charge relay system (By similarity). FT ACT_SITE 1550 1550 Charge relay system (By similarity). FT ACT_SITE 1610 1610 Charge relay system (By similarity). FT CARBOHYD 183 183 N-linked (GlcNAc...) (Potential). FT CARBOHYD 347 347 N-linked (GlcNAc...) (Potential). FT CARBOHYD 433 433 N-linked (GlcNAc...) (Potential). FT CARBOHYD 905 905 N-linked (GlcNAc...) (Potential). FT CARBOHYD 982 982 N-linked (GlcNAc...) (Potential). FT CARBOHYD 1134 1134 N-linked (GlcNAc...) (Potential). FT CARBOHYD 2301 2301 N-linked (GlcNAc...) (Potential). FT CARBOHYD 2305 2305 N-linked (GlcNAc...) (Potential). FT CARBOHYD 2457 2457 N-linked (GlcNAc...) (Potential). FT CARBOHYD 2485 2485 N-linked (GlcNAc...) (Potential). FT CARBOHYD 2665 2665 N-linked (GlcNAc...) (Potential). FT CARBOHYD 2704 2704 N-linked (GlcNAc...) (Potential). FT CARBOHYD 2714 2714 N-linked (GlcNAc...) (Potential). FT DISULFID 283 310 By similarity. FT DISULFID 340 401 By similarity. FT DISULFID 354 385 By similarity. FT DISULFID 372 396 By similarity. FT DISULFID 465 565 By similarity. FT DISULFID 582 613 By similarity. SQ SEQUENCE 3391 AA; 379884 MW; 5EE11A74081C5177 CRC64; MNDQRKEAKN TPFNMLKRER NRVSTVQQLT KRFSLGMLQG RGPLKLYMAL VAFLRFLTIP PTAGILKRWG TIKKSKAINV LRGFRKEIGR MLNILNRRRR SAGMIIMLIP TVMAFHLTTR NGEPHMIVSR QEKGKSLLFK TEVGVNMCTL MAMDLGELCE DTITYKCPLL RQNEPEDIDC WCNSTSTWVT YGTCTTMGEH RREKRSVALV PHVGMGLETR TETWMSSEGA WKHVQRIETW ILRHPGFTMM AAILAYTIGT THFQRALILI LLTAVTPSMT MRCIGMSNRD FVEGVSGGSW VDIVLEHGSC VTTMAKNKPT LDFELIKTEA KQPATLRKYC IEAKLTNTTT ESRCPTQGEP SLNEEQDKRF VCKHSMVDRG WGNGCGLFGK GGIVTCAMFR CKKNMEGKVV QPENLEYTIV ITPHSGEEHA VGNDTGKHGK EIKITPQSSI TEAELTGYGT ITMECSPRTG LDFNEIVLLQ MENKAWLVHR QWFLDLPLPW LPGADTQGSN WIQKETLVTF KNPHAKKQDV VVLGSQEGAM HTALTGATEI QMSSGNLLFT GHLKCRLRMD KLQLKGMSYS MCTGKFKVVK EIAETQHGTI VIRVQYEGDG SPCKIPFEIM DLEKRHVLGR LITVNPIVTE KDSPVNIEAE PPFGDSYIII GVEPGQLKLN WFKKGSSIGQ MFETTMRGAK RMAILGDTAW DFGSLGGVFT SIGKALHQVF GAIYGAAFSG VSWTMKILIG VIITWIGMNS RSTSLSVTLV LVGIVTLYLG VMVQADSGCV VSWKNKELKC GSGIFITDNV HTWTEQYKFQ PESPSKLASA IQKAHEEDIC GIRSVTRLEN LMWKQITPEL NHILSENEVK LTIMTGDIKG IMQAGKRSLR PQPTELKYSW KTWGKAKMLS TESHNQTFFI DGPETAECPN TNRAWNSLEV EDYGFGVFTT NIWLKLKEKQ DVFCDSKLMS AAIKDNRAVH ADMGYWIESA LNDTWKIEKA SFIEVKNCHW PKSHTLWSNG VLESEMIIPK NLAGPVSKHN YRPGYHTQIT GPWHLGKLEM DFDFCDGTTV VVTEDCGNRG PSLRTTTASG KLITEWCCRS CTLPPLRYRG EDGCWYGMEI RPLKEKEENL VNSLVTAGHG QVDNFSLGVL GMALFLEEML RTRVGTKHAI LLVAVSFVTL IIGNRSFRDL GRVMVMVGAT MTDDIGMGVT YLALLAAFKV RPTFAAGLLL RKLTSKELMM TTIGIVLSSQ STIPETILEL TDALALGMMV LKMVRNMEKY QLAVTIMAIL CVPNAVILQN AWKVSCTILA VVSVSPLFLT SSQQKTDWIP LALTIKGLNP TAIFLTTLSR TSKKRSWPLN EAIMAVGMVS ILASSLLKND IPMTGPLVAG GLLTVCYVLT GRSADLELER AADVKWEDQA EISGSSPILS ITISEDGSMS IKNEEEEQTL TILIRRGLLV ISGLFPVSIP ITAAAWYLWE VKKQRAGVLW DVPSPPPMGK AELEDGAYRI KQKGILGYSQ IGAGVYKEGT FHTMWHVTRG AVLMHKGKRI EPSWADVKKD LISYGGGWKL EGEWKEGEEV QVLALDPGKN PRAVQTKPGL FKTNAGTIGA VSLDFSPGTS GSPIIDKKGK VVGLYGNGVV TRSGAYVSAI AQTEKSIEDN PEIEDDIFRK RRLTIMDLHP GAGKTKRYLP AIVREAIKRG LRTLILAPTR VVAAEMEEAL RGLPIRYQTP AIRAEHTGRE IVDLMCHATF TMRLLSPVRV PNYNLIIMDE AHFTDPASIA ARGYISTRVE MGEAAGIFMT ATPPGSRDPF PQSNAPIIDE EREIPERSWN SGHEWVTDFK GKTVWFVPSI KAGNDIAACL RKNGKKVIQL SRKTFDSEYV KTRTNDWDFV VTTDISEMGA NFKAERVIDP RRCMKPVILT DGEERVILAG PMPVTHSSAA QRRGRIGRNP KNENDQYIYM GEPLENDEDC AHWKEAKMLL DNINTPEGII PSMFEPEREK VDAIDGEYRL RGEARTTFVD LMRRGDLPVW LAYRVAAEGI NYADRRWCFD GVKNNQILEE NVEVEIWTKE GERKKLKPRW LDARIYSDPL ALKEFKEFAA GRKSLTLNLI TEMGRLPTFM TQKARNALDN LAVLHTAEAG GRAYNHALSE LPETLETLLL LTLLATVTGG IFLFLMSARG IGKMTLGMCC IITASILLWY AQIQPHWIAA SIILEFFLIV LLIPEPEKQR TPQDNQLTYV VIAILTVVAA TMANEMGFLE KTKKDLGLGS IATQQPESNI LDIDLRPASA WTLYAVATTF VTPMLRHSIE NSSVNVSLTA IANQATVLMG LGKGWPLSKM DIGVPLLAIG CYSQVNPTTL TAALFLLVAH YAIIGPALQA KASREAQKRA AAGIMKNPTV DGITVIDLDP IPYDPKFEKQ LGQVMLLVLC VTQVLMMRTT WALCEALTLA TGPISTLSEG NPGRFWNTTI AVSMANIFRG SYLAGAGLLF SIMKNTTNTR RVTGNIGETL GEKWKSRLNA LGKSEFQIYK KSGIQEVDRT LAKEGIKRGE TDHHAVSRGS AKLRWFVERN MVTPEGKVVD LGCGRGGWSY YCGGLKNVRE VKGLTKGGPG HEEPIPMSTY GWNLVRLQSG VDVFFIPPEK CDTLLCDIGE SSPNPTVEAG RTLRVLNLVE NWLNNNTQFC IKVLNPYMPS VIEKMEALQR KYGGALVRNP LSRNSTHEMY WVSNASGNIV SSVNMISRML INRFTMRYKK ATYEPDVDLG SGTRNIGIES EIPNLDIIGK RIEKIKQEHE TSWHYDQDHP YKTWAYHGSY ETKQTGSASS MVNGVFRLLT KPWDVVPMVT QMAMTDTTPF GQQRVFKEKV DTRTQEPKEG TKKLMKITAE WLWKELGKKK TPRMCTREEF TRKVRSNAAL GAIFTDENKW KSAREAVEDS RFWELVDKER NLHLEGKCET CVYNIMGKRE KKLGEFGKAK GSRAIWYMWL GARFLEFEAL GFLNEDHWFS RENSLSGVEG EGLHKLGYIL RDVSKKEGGA MYADDTAGWD TRITLEDLKN EEMVTNHMEG EHKKLAEAIF KLTYQNKVVR VQRPTPRGTV MDIISRRDQR GSGQVGTYGL NTFTNMEAQL IRQMEGEGVF KSIQHLTITE EIAVQNWLAR VGRERLSRMA ISGDDCVVKP LDDRLPSALT ALNDMGKIRK DIQQWEPSRG WNDWTQVPFC SHHFHELIMK DGRVLVVPCR NQDELIGRAR ISQGAGWSLR ETACLGKSYA QMWSLMYFHR RDLRLAANAI CSAVPSHWVP TSRTTWSIHA KHEWMTTEDM LTVWNRVWIQ ENPWMEDKTP VESWEEIPYL GKREDQWCGS LIGLTSRATW AKNIQAAINQ VRSLIGNEEY TDYMPSMKRF RREEEEAGVL W //