ID 1A_TAV Reviewed; 993 AA. AC P28931; DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-1992, sequence version 1. DT 29-MAY-2013, entry version 52. DE RecName: Full=Replication protein 1a; DE Includes: DE RecName: Full=ATP-dependent helicase; DE EC=3.6.4.-; DE Includes: DE RecName: Full=Methyltransferase; DE EC=2.1.1.-; GN ORFNames=ORF1a; OS Tomato aspermy virus (TAV). OC Viruses; ssRNA positive-strand viruses, no DNA stage; Bromoviridae; OC Cucumovirus. OX NCBI_TaxID=12315; OH NCBI_TaxID=4627; Canna. OH NCBI_TaxID=41568; Chrysanthemum morifolium (Florist's daisy) (Dendranthema grandiflorum). OH NCBI_TaxID=4688; Lilium. OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=V; RX PubMed=1895056; RA Bernal J.J., Moriones E., Garcia-Arenal F.; RT "Evolutionary relationships in the cucumoviruses: nucleotide sequence RT of tomato aspermy virus RNA 1."; RL J. Gen. Virol. 72:2191-2195(1991). CC -!- FUNCTION: Involved in the virus replication. Contains a helicase CC domain and a methyltransferase domain. The methyltransferase CC domain is probably involved in viral RNA capping. Involved in the CC formation of ER membrane spherular invaginations in which RNA CC replication complexes form (By similarity). CC -!- SUBUNIT: Interacts with RNA-directed RNA polymerase 2a (By CC similarity). CC -!- SUBCELLULAR LOCATION: Host endoplasmic reticulum membrane; CC Peripheral membrane protein (By similarity). CC -!- SIMILARITY: Belongs to the bromoviridae replication protein 1a CC family. CC -!- SIMILARITY: Contains 1 (+)RNA virus helicase ATP-binding domain. CC -!- SIMILARITY: Contains 1 (+)RNA virus helicase C-terminal domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D10044; BAA00936.1; -; Genomic_RNA. DR PIR; JQ1370; P1VXTA. DR RefSeq; NP_620760.1; NC_003837.1. DR GeneID; 962652; -. DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW. DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0006396; P:RNA processing; IEA:InterPro. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR021002; 1a_necrotic_phenotyp-det_dom. DR InterPro; IPR022184; CMV_1a_C. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR002588; Tymovirus_MeTrfase. DR Pfam; PF12467; CMV_1a; 1. DR Pfam; PF12503; CMV_1a_C; 1. DR Pfam; PF01443; Viral_helicase1; 1. DR Pfam; PF01660; Vmethyltransf; 1. DR SUPFAM; SSF52540; SSF52540; 2. DR PROSITE; PS51657; PSRV_HELICASE; 1. PE 3: Inferred from homology; KW ATP-binding; Complete proteome; Helicase; Host endoplasmic reticulum; KW Host membrane; Hydrolase; Membrane; Methyltransferase; KW Nucleotide-binding; Transferase. FT CHAIN 1 993 Replication protein 1a. FT /FTId=PRO_0000083266. FT DOMAIN 682 838 (+)RNA virus helicase ATP-binding. FT DOMAIN 839 993 (+)RNA virus helicase C-terminal. FT NP_BIND 714 721 ATP (Potential). FT REGION 50 408 Methyltransferase. FT REGION 711 975 ATP-dependent helicase. SQ SEQUENCE 993 AA; 112062 MW; 516926DD910FF4EA CRC64; MAASAFNIHK LVASHGDKGL LASALVDKLA HEQLEEQVQH QRRGLKVYIR NALDVKDSEI IRDRYGGKYD LHLTQQEQAP HGLAGALRLC ELLDCLDSFP RTGLRQDLVL DFGGSWVTHY LRGHNVHCCS PCLGIRDKMR HTERLMTMRK VIVNDPDTFE SRVPDFCTLP AEDCKVQAHF VISIHGGYDM GFKGLCRAMH AHGTTFLKGT MMFDGAMLFD TEGYLADLKC KWKKIKPRTY ESEDQTPLLS RISDNLTTTI KDHTLIAFDF VDESTLSYVH KWENVKSFLT DQTYSYKGMT YGLERCLIQH GIMTYKIIAV PGTCPPELIR HCIWFTSLKD YVGLKIPVSQ DLVEWKTVRI LISTLRETEE IAMRCYSDKK NWLEQFKVIL GVLSSKSSTI VINGMAMQAG ERIDTSDYHY IGLAILLHTR MKYEQLGRMY DMWNSTFIRK FFASLTRPMR VFLSACVKTL FPTLRPRDEK EFLVKLSTFV TFNEVCQVDL DAEWDVISSA AFTAEMAVED GKRLAEDRKQ KAEAASQIPV DEIPDDTAES SDDTPREADT NQKSEPSSPE LETLSTQTRS PITRLAQRAS SMLEYSAYEA QLHDNAVSNL DRMWCMAGGD KKNNRLESNV KFVFETYHIV DPLVNVHFPT GRWLYRVPEG ISYSVGFNEH GIGPKADGEL YIVNADCVIS NSKCLADTTL QYLAPTGTIS LVDGVAGCGK TTAIKKMFNP ATDVIVTANK KSALDVRQAL FNCTDSKEAT TFVRTADSIL MNDTNEVQRV LVDEVVLLHF GQLCAVMSKL KAVRAICFGD SEQIAFCSRD ASFDMRHSTI IPDETDTADT TFRSPQDVIK VVKCMASKAL KKGTHSKYAS WVSQSKVQRS VSSKAVASVT MVDLTEDRFY ITMTQADKTA LRTRARELNM SNDFIEHRIK TTHESQGVSE DHVTLVRLKT TKCDLFKAFK YCLVAVTRHK KTFRYEHVGK LDGDLIAECL ARV //