ID PUA1_MOUSE STANDARD; PRT; 457 AA. AC P28650; DT 01-DEC-1992 (REL. 24, CREATED) DT 01-FEB-1996 (REL. 33, LAST SEQUENCE UPDATE) DT 01-NOV-1997 (REL. 35, LAST ANNOTATION UPDATE) DE ADENYLOSUCCINATE SYNTHETASE, MUSCLE ISOZYME (EC 6.3.4.4) (IMP-- DE ASPARTATE LIGASE). GN ADSS1. OS MUS MUSCULUS (MOUSE). OC EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; TETRAPODA; MAMMALIA; OC EUTHERIA; RODENTIA. RN [1] RP SEQUENCE FROM N.A. RC TISSUE=MUSCLE; RX MEDLINE; 92042206. RA GUICHERIT O.M., RUDOLPH F.B., KELLEMS R.E., COOPER B.F.; RL J. BIOL. CHEM. 266:22582-22587(1991). RN [2] RP REVISIONS. RX MEDLINE; 94140883. RA GUICHERIT O.M., COOPER B.F., RUDOLPH F.B., KELLEMS R.E.; RL J. BIOL. CHEM. 269:4488-4496(1994). CC -!- FUNCTION: PLAYS AN IMPORTANT ROLE IN THE DE NOVO PATHWAY OF PURINE CC NUCLEOTIDE BIOSYNTHESIS. CC -!- CATALYTIC ACTIVITY: GTP + IMP + L-ASPARTATE = GDP + CC ORTHOPHOSPHATE + ADENYLOSUCCINATE. CC -!- PATHWAY: FIRST COMMITTED STEP IN AMP BIOSYNTHESIS. CC -!- SUBCELLULAR LOCATION: CYTOPLASMIC, AND PARTIALLY ASSOCIATED WITH CC PARTICULATE FRACTIONS. CC -!- TISSUE SPECIFICITY: HIGH LEVEL IN MUSCLE. CC -!- SIMILARITY: WITH OTHER ADENYLOSUCCINATE SYNTHETASES. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M74495; G404751; -. DR PIR; A39317; AJMSDS. DR MGD; MGI:87947; ADSS1. DR PROSITE; PS00513; ADENYLOSUCCIN_SYN_2; 1. DR PROSITE; PS01266; ADENYLOSUCCIN_SYN_1; 1. KW PURINE BIOSYNTHESIS; LIGASE; GTP-BINDING; MULTIGENE FAMILY. FT NP_BIND 42 48 GTP (POTENTIAL). FT ACT_SITE 174 174 BY SIMILARITY. FT ACT_SITE 181 181 BY SIMILARITY. SQ SEQUENCE 457 AA; 50254 MW; 26D05DAB CRC32; MSGTRASNDR PPGTGGVKRG RLQQEAAATG SRVTVVLGAQ WGDEGKGKVV DLLATDADIV SRCQGGNNAG HTVVVDGKEY DFHLLPSGII NTKAVSFIGN GVVIHLPGLF EEAEKNEKKG LKDWEKRLII SDRAHLVFDF HQAVDGLQEV QRQAQEGKNI GTTKKGIGPT YSSKAARTGL RICDLLSDFD EFSARFKNLA HQHQSMFPTL EIDVEGQLKR LKGFAERIRP MVRDGVYFMY EALHGPPKKV LVEGANAALL DIDFGTYPFV TSSNCTVGGV CTGLGIPPQN IGDVYGVVKA YTTRVGIGAF PTEQINEIGD LLQNRGHEWG VTTGRKRRCG WLDLMILRYA HMVNGFTALA LTKLDILDVL SEIKVGISYK LNGKRIPYFP ANQEILQKVE VEYETLPGWK ADTTGARKWE DLPPQAQSYV RFVENHMGVA VKWVGVGKSR ESMIQLF //