ID RAA5C_ARATH Reviewed; 214 AA. AC P28187; Q1ECF0; DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-1992, sequence version 1. DT 03-APR-2013, entry version 106. DE RecName: Full=Ras-related protein RABA5c; DE Short=AtRABA5c; DE AltName: Full=Ras-related protein Ara-4; DE AltName: Full=Ras-related protein Rab11F; DE Short=AtRab11F; GN Name=RABA5C; Synonyms=ARA-4, RAB11F; OrderedLocusNames=At2g43130; GN ORFNames=F14B2.7; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Columbia, cv. En-1, cv. Est, cv. Landsberg erecta, and RC cv. Lapalmam; TISSUE=Leaf; RX PubMed=1748311; DOI=10.1016/0378-1119(91)90442-E; RA Anai T., Hasegawa K., Watanabe Y., Uchimiya H., Ishizaki R., RA Matsui M.; RT "Isolation and analysis of cDNAs encoding small GTP-binding proteins RT of Arabidopsis thaliana."; RL Gene 108:259-264(1991). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=10617197; DOI=10.1038/45471; RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., RA Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., RA Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., RA Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., RA Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., RA Venter J.C.; RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis RT thaliana."; RL Nature 402:761-768(1999). RN [3] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Resource (TAIR); RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=19423640; DOI=10.1093/dnares/dsp009; RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., RA Seki M., Shinozaki K.; RT "Analysis of multiple occurrences of alternative splicing events in RT Arabidopsis thaliana using novel sequenced full-length cDNAs."; RL DNA Res. 16:155-164(2009). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.; RT "Arabidopsis ORF clones."; RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases. RN [6] RP FUNCTION, AND MUTAGENESIS OF GLN-71 AND ASN-125. RX PubMed=8013629; DOI=10.1016/0014-5793(94)80696-9; RA Anai T., Matsui M., Nomura N., Ishizaki R., Uchimiya H.; RT "In vitro mutation analysis of Arabidopsis thaliana small GTP-binding RT proteins and detection of GAP-like activities in plant cells."; RL FEBS Lett. 346:175-180(1994). RN [7] RP SUBCELLULAR LOCATION. RX PubMed=8676856; DOI=10.1007/s004380050106; RA Ueda T., Anai T., Tsukaya H., Hirata A., Uchimiya H.; RT "Characterization and subcellular localization of a small GTP-binding RT protein (Ara-4) from Arabidopsis: conditional expression under control RT of the promoter of the gene for heat-shock protein HSP81-1."; RL Mol. Gen. Genet. 250:533-539(1996). RN [8] RP INDUCTION BY ABSCISIC ACID. RX PubMed=10341440; DOI=10.1046/j.1365-313X.1999.00423.x; RA Jeannette E., Rona J.P., Bardat F., Cornel D., Sotta B., Miginiac E.; RT "Induction of RAB18 gene expression and activation of K+ outward RT rectifying channels depend on an extracellular perception of ABA in RT Arabidopsis thaliana suspension cells."; RL Plant J. 18:13-22(1999). RN [9] RP INDUCTION. RX PubMed=12401218; DOI=10.1016/S0167-4781(02)00502-X; RA Ciereszko I., Kleczkowski L.A.; RT "Effects of phosphate deficiency and sugars on expression of rab18 in RT Arabidopsis: hexokinase-dependent and okadaic acid-sensitive RT transduction of the sugar signal."; RL Biochim. Biophys. Acta 1579:43-49(2002). RN [10] RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY. RX PubMed=11532937; DOI=10.1093/emboj/20.17.4730; RA Ueda T., Yamaguchi M., Uchimiya H., Nakano A.; RT "Ara6, a plant-unique novel type Rab GTPase, functions in the RT endocytic pathway of Arabidopsis thaliana."; RL EMBO J. 20:4730-4741(2001). RN [11] RP GENE FAMILY, AND NOMENCLATURE. RX PubMed=12644670; DOI=10.1104/pp.013052; RA Vernoud V., Horton A.C., Yang Z., Nielsen E.; RT "Analysis of the small GTPase gene superfamily of Arabidopsis."; RL Plant Physiol. 131:1191-1208(2003). CC -!- FUNCTION: Intracellular vesicle trafficking and protein transport. CC Binds GTP and GDP and possesses intrinsic GTPase activity. CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane. Cell membrane; CC Lipid-anchor (Probable). CC -!- TISSUE SPECIFICITY: Expressed in roots and actively dividing CC cells. CC -!- INDUCTION: By abscisic acid (ABA), phosphate (Pi) deprivation, CC cold, sucrose, mannose, and water stress. CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D01026; BAA00831.1; -; mRNA. DR EMBL; AC004450; AAC64302.1; -; Genomic_DNA. DR EMBL; CP002685; AEC10212.1; -; Genomic_DNA. DR EMBL; BT025784; ABF83674.1; -; mRNA. DR EMBL; AK317205; BAH19889.1; -; mRNA. DR IPI; IPI00531444; -. DR PIR; JS0641; JS0641. DR RefSeq; NP_181842.1; NM_129875.3. DR UniGene; At.259; -. DR ProteinModelPortal; P28187; -. DR SMR; P28187; 10-174. DR STRING; 3702.AT2G43130.1-P; -. DR PaxDb; P28187; -. DR PRIDE; P28187; -. DR EnsemblPlants; AT2G43130.1; AT2G43130.1; AT2G43130. DR GeneID; 818915; -. DR GenomeReviews; CT485783_GR; AT2G43130. DR KEGG; ath:AT2G43130; -. DR TAIR; At2g43130; -. DR eggNOG; COG1100; -. DR HOGENOM; HOG000233968; -. DR InParanoid; P28187; -. DR KO; K07976; -. DR OMA; YDITRSS; -. DR PhylomeDB; P28187; -. DR ProtClustDB; CLSN2683060; -. DR Genevestigator; P28187; -. DR GermOnline; AT2G43130; Arabidopsis thaliana. DR GO; GO:0005829; C:cytosol; IDA:TAIR. DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005795; C:Golgi stack; IDA:TAIR. DR GO; GO:0005798; C:Golgi-associated vesicle; IDA:TAIR. DR GO; GO:0005886; C:plasma membrane; IDA:TAIR. DR GO; GO:0005802; C:trans-Golgi network; IDA:TAIR. DR GO; GO:0005525; F:GTP binding; IDA:UniProtKB. DR GO; GO:0003924; F:GTPase activity; IDA:UniProtKB. DR GO; GO:0048219; P:inter-Golgi cisterna vesicle-mediated transport; TAS:TAIR. DR GO; GO:0015031; P:protein transport; IEA:InterPro. DR GO; GO:0009408; P:response to heat; IEP:TAIR. DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. DR InterPro; IPR005225; Small_GTP-bd_dom. DR InterPro; IPR001806; Small_GTPase. DR InterPro; IPR003579; Small_GTPase_Rab_type. DR Pfam; PF00071; Ras; 1. DR PRINTS; PR00449; RASTRNSFRMNG. DR SMART; SM00175; RAB; 1. DR TIGRFAMs; TIGR00231; small_GTP; 1. DR PROSITE; PS51419; RAB; 1. PE 1: Evidence at protein level; KW Cell membrane; Complete proteome; Golgi apparatus; GTP-binding; KW Lipoprotein; Membrane; Nucleotide-binding; Prenylation; KW Reference proteome. FT CHAIN 1 214 Ras-related protein RABA5c. FT /FTId=PRO_0000121291. FT NP_BIND 19 26 GTP (By similarity). FT NP_BIND 67 71 GTP (By similarity). FT NP_BIND 125 128 GTP (By similarity). FT NP_BIND 155 156 GTP (By similarity). FT MOTIF 41 49 Effector region (By similarity). FT LIPID 211 211 S-geranylgeranyl cysteine (By FT similarity). FT LIPID 212 212 S-geranylgeranyl cysteine (By FT similarity). FT MUTAGEN 71 71 Q->L: Loss of GTPase activity. FT MUTAGEN 125 125 N->I: Decreases GTP-binding efficiency. SQ SEQUENCE 214 AA; 23981 MW; B5C11E754C14535C CRC64; MSDDDERGEE YLFKIVIIGD SAVGKSNLLT RYARNEFNPN SKATIGVEFQ TQSMLIDGKE VKAQIWDTAG QERFRAVTSA YYRGAVGALV VYDITRSSTF ENVGRWLDEL NTHSDTTVAK MLIGNKCDLE SIRAVSVEEG KSLAESEGLF FMETSALDST NVKTAFEMVI REIYSNISRK QLNSDSYKEE LTVNRVSLVK NENEGTKTFS CCSR //