ID DCMB_MOOTH STANDARD; PRT; 674 AA. AC P27989; DT 01-AUG-1992 (Rel. 23, Created) DT 01-AUG-1992 (Rel. 23, Last sequence update) DT 29-MAR-2004 (Rel. 43, Last annotation update) DE Carbon monoxide dehydrogenase/acetyl CoA synthase beta subunit DE (EC 1.2.7.4) (EC 1.2.99.2) (Carbon monoxide dehydrogenase subunit) DE (CODH/ACS) (CODH subunit). OS Moorella thermoacetica (Clostridium thermoaceticum). OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacteriales; OC Thermoanaerobacteriaceae; Moorella group; Moorella. OX NCBI_TaxID=1525; RN [1] RP SEQUENCE FROM N.A. RX MEDLINE=92084676; PubMed=1748656; RA Morton T.A., Runquist J.A., Ragsdale S.W., Shanmugasundaram T., RA Wood H.G., Ljungdahl L.G.; RT "The primary structure of the subunits of carbon monoxide RT dehydrogenase/acetyl-CoA synthase from Clostridium thermoaceticum."; RL J. Biol. Chem. 266:23824-23828(1991). RN [2] RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). RX PubMed=12386327; RA Doukov T.I., Iverson T.M., Seravalli J., Ragsdale S.W., Drennan C.L.; RT "A Ni-Fe-Cu center in a bifunctional carbon monoxide RT dehydrogenase/acetyl-CoA synthase."; RL Science 298:567-572(2002). RN [3] RP X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS). RX PubMed=12627225; RA Darnault C., Volbeda A., Kim E.J., Legrand P., Vernede X., RA Lindahl P.A., Fontecilla-Camps J.C.; RT "Ni-Zn-[Fe4-S4] and Ni-Ni-[Fe4-S4] clusters in closed and open RT subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase."; RL Nat. Struct. Biol. 10:271-279(2003). CC -!- FUNCTION: The beta subunit (this protein) generates CO from CO(2), CC while the alpha subunit combines the CO with CoA and a methyl CC group to form acetyl-CoA. The methyl group, which is incorporated CC into acetyl-CoA, is transferred to the alpha subunit by a CC corrinoid iron-sulfur protein. CC -!- CATALYTIC ACTIVITY: CO + H(2)O + oxidized ferredoxin = CO(2) + CC reduced ferredoxin. CC -!- CATALYTIC ACTIVITY: CO + H(2)O + A = CO(2) + AH(2). CC -!- COFACTOR: Binds 1 Nickel-iron-sulfur cluster per subunit and 2 CC 4Fe-4S clusters per homodimer. CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M62727; AAA23228.1; -. DR PIR; A41670; A41670. DR PDB; 1MJG; 28-JAN-03. DR PDB; 1OAO; 11-APR-03. DR InterPro; IPR004137; Prismane. DR Pfam; PF03063; Prismane; 1. DR PIRSF; PIRSF005023; CODH; 1. DR TIGRFAMs; TIGR01702; CO_DH_cata; 1. KW Carbon dioxide fixation; Oxidoreductase; Metal-binding; Iron; Nickel; KW Iron-sulfur; 4Fe-4S; Electron transport; 3D-structure. FT METAL 59 59 Iron-sulfur 1 (4Fe-4S) (shared with FT dimeric partner). FT METAL 67 67 Iron-sulfur 1 (4Fe-4S) (shared with FT dimeric partner). FT METAL 68 68 Iron-sulfur 2 (4Fe-4S). FT METAL 71 71 Iron-sulfur 2 (4Fe-4S). FT METAL 76 76 Iron-sulfur 2 (4Fe-4S). FT METAL 90 90 Iron-sulfur 2 (4Fe-4S). FT METAL 283 283 Ni-4Fe-4S cluster. FT METAL 317 317 Ni-4Fe-4S cluster. FT METAL 355 355 Ni-4Fe-4S cluster. FT METAL 470 470 Ni-4Fe-4S cluster. FT METAL 500 500 Ni-4Fe-4S cluster. FT METAL 550 550 Ni-4Fe-4S cluster. FT TURN 7 8 FT TURN 22 23 FT STRAND 28 28 FT HELIX 31 42 FT TURN 43 44 FT HELIX 48 55 FT HELIX 60 63 FT TURN 64 65 FT STRAND 67 68 FT TURN 72 73 FT STRAND 76 77 FT TURN 84 85 FT TURN 89 90 FT HELIX 94 128 FT TURN 129 130 FT TURN 133 134 FT HELIX 140 149 FT TURN 150 151 FT TURN 155 156 FT HELIX 159 175 FT TURN 178 179 FT HELIX 184 187 FT TURN 188 189 FT HELIX 192 200 FT TURN 201 202 FT HELIX 208 219 FT TURN 221 222 FT HELIX 227 256 FT STRAND 262 266 FT HELIX 268 270 FT TURN 273 274 FT STRAND 276 281 FT HELIX 285 296 FT TURN 297 298 FT HELIX 299 304 FT TURN 305 306 FT STRAND 310 315 FT HELIX 316 326 FT STRAND 330 332 FT HELIX 334 336 FT HELIX 337 341 FT TURN 342 343 FT STRAND 348 351 FT TURN 358 359 FT HELIX 360 364 FT TURN 365 368 FT STRAND 370 373 FT STRAND 375 375 FT TURN 376 377 FT STRAND 378 378 FT TURN 381 382 FT STRAND 384 385 FT HELIX 390 392 FT HELIX 393 412 FT TURN 413 413 FT STRAND 425 429 FT HELIX 433 441 FT TURN 442 443 FT HELIX 448 458 FT TURN 459 459 FT STRAND 463 467 FT TURN 473 474 FT STRAND 477 477 FT TURN 478 478 FT HELIX 479 490 FT TURN 491 492 FT STRAND 493 498 FT HELIX 499 507 FT TURN 508 511 FT HELIX 513 515 FT HELIX 516 519 FT HELIX 522 534 FT TURN 535 536 FT TURN 538 539 FT STRAND 544 548 FT TURN 551 552 FT HELIX 553 567 FT TURN 568 568 FT HELIX 571 573 FT STRAND 576 580 FT TURN 581 582 FT HELIX 586 597 FT TURN 598 599 FT STRAND 601 604 FT TURN 609 612 FT HELIX 614 621 FT TURN 622 622 FT HELIX 623 627 FT TURN 628 628 FT STRAND 631 634 FT HELIX 638 665 FT TURN 666 667 SQ SEQUENCE 674 AA; 72924 MW; 54BA3D816C25F9FC CRC64; MPRFRDLSHN CRPSEAPRVM EPKNRDRTVD PAVLEMLVKS KDDKVITAFD RFVAQQPQCK IGYEGICCRF CMAGPCRIKA TDGPGSRGIC GASAWTIVAR NVGLMILTGA AAHCEHGNHI AHALVEMAEG KAPDYSVKDE AKLKEVCRRV GIEVEGKSVL ELAQEVGEKA LEDFRRLKGE GEATWLMTTI NEGRKEKFRT HNVVPFGIHA SISELVNQAH MGMDNDPVNL VFSAIRVALA DYTGEHIATD FSDILFGTPQ PVVSEANMGV LDPDQVNFVL HGHNPLLSEI IVQAAREMEG EAKAAGAKGI NLVGICCTGN EVLMRQGIPL VTSFASQELA ICTGAIDAMC VDVQCIMPSI SAVAECYHTR IITTADNAKI PGAYHIDYQT ATAIESAKTA IRMAIEAFKE RKESNRPVYI PQIKNRVVAG WSLEALTKLL ATQNAQNPIR VLNQAILDGE LAGVALICGC NNLKGFQDNS HLTVMKELLK NNVFVVATGC SAQAAGKLGL LDPANVETYC GDGLKGFLKR LGEGANIEIG LPPVFHMGSC VDNSRAVDLL MAMANDLGVD TPKVPFVASA PEAMSGKAAA IGTWWVSLGV PTHVGTMPPV EGSDLIYSIL TQIASDVYGG YFIFEMDPQV AARKILDALE YRTWKLGVHK EVAERYETKL CQGY //