ID U2AF2_HUMAN Reviewed; 475 AA. AC P26368; DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 4. DT 24-JUL-2007, entry version 88. DE Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa DE subunit) (U2 snRNP auxiliary factor large subunit) (hU2AF(65)). GN Name=U2AF2; Synonyms=U2AF65; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX MEDLINE=92168111; PubMed=1538748; DOI=10.1038/355609a0; RA Zamore P.D., Patton J.G., Green M.R.; RT "Cloning and domain structure of the mammalian splicing factor U2AF."; RL Nature 355:609-614(1992). RN [2] RP PROTEIN SEQUENCE OF 2-9; 196-203; 277-286 AND 463-471, CLEAVAGE OF RP INITIATOR METHIONINE, ACETYLATION AT SER-2, AND MASS SPECTROMETRY. RC TISSUE=Colon carcinoma; RA Bienvenut W.V., Heiserich L., Boulahbel H., Gottlieb E.; RL Unpublished observations (NOV-2006). RN [3] RP PROTEIN SEQUENCE OF 261-286, MASS SPECTROMETRY, AND INTERACTION WITH RP THE SPLICEOSOME. RX MEDLINE=22166132; PubMed=12176931; DOI=10.1101/gr.473902; RA Rappsilber J., Ryder U., Lamond A.I., Mann M.; RT "Large-scale proteomic analysis of the human spliceosome."; RL Genome Res. 12:1231-1245(2002). RN [4] RP INTERACTION WITH SFRS2IP. RX MEDLINE=98107652; PubMed=9447963; RA Zhang W.-J., Wu J.Y.; RT "Sip1, a novel RS domain-containing protein essential for pre-mRNA RT splicing."; RL Mol. Cell. Biol. 18:676-684(1998). RN [5] RP INTERACTION WITH SF1. RX MEDLINE=99380171; PubMed=10449420; DOI=10.1093/emboj/18.16.4549; RA Wang X., Bruderer S., Rafi Z., Xue J., Milburn P.J., Kraemer A., RA Robinson P.J.; RT "Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent RT protein kinase regulates spliceosome assembly."; RL EMBO J. 18:4549-4559(1999). RN [6] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, AND MASS RP SPECTROMETRY. RC TISSUE=Hepatocyte; RX PubMed=16097034; DOI=10.1002/pmic.200401217; RA Gevaert K., Staes A., Van Damme J., De Groot S., Hugelier K., RA Demol H., Martens L., Goethals M., Vandekerckhove J.; RT "Global phosphoproteome analysis on human HepG2 hepatocytes using RT reversed-phase diagonal LC."; RL Proteomics 5:3589-3599(2005). RN [7] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-53; SER-55 AND RP SER-79, AND MASS SPECTROMETRY. RC TISSUE=Epithelium; RX PubMed=17081983; DOI=10.1016/j.cell.2006.09.026; RA Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., RA Mann M.; RT "Global, in vivo, and site-specific phosphorylation dynamics in RT signaling networks."; RL Cell 127:635-648(2006). RN [8] RP STRUCTURE BY NMR OF 148-237. RX MEDLINE=99380169; PubMed=10449418; DOI=10.1093/emboj/18.16.4523; RA Ito T., Muto Y., Green M.R., Yokoyama S.; RT "Solution structures of the first and second RNA-binding domains of RT human U2 small nuclear ribonucleoprotein particle auxiliary factor RT (U2AF(65))."; RL EMBO J. 18:4523-4534(1999). RN [9] RP STRUCTURE BY NMR OF 372-475 IN COMPLEX WITH SF1, AND MUTAGENESIS OF RP 387-GLU-GLU-388; 391-ASP--GLU-393; 396-GLU-GLU-397 AND PHE-454. RX MEDLINE=22605544; PubMed=12718882; DOI=10.1016/S1097-2765(03)00115-1; RA Selenko P., Gregorovic G., Sprangers R., Stier G., Rhani Z., RA Kraemer A., Sattler M.; RT "Structural basis for the molecular recognition between human splicing RT factors U2AF65 and SF1/mBBP."; RL Mol. Cell 11:965-976(2003). RN [10] RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 90-112 IN COMPLEX WITH U2AF1, RP AND MUTAGENESIS OF TRP-92; PRO-96 AND PRO-104. RX MEDLINE=21435806; PubMed=11551507; DOI=10.1016/S0092-8674(01)00480-9; RA Kielkopf C.L., Rodionova N.A., Green M.R., Burley S.K.; RT "A novel peptide recognition mode revealed by the X-ray structure of a RT core U2AF35/U2AF65 heterodimer."; RL Cell 106:595-605(2001). CC -!- FUNCTION: Necessary for the splicing of pre-mRNA. Binds to the CC polypyrimidine tract of introns early during spliceosome assembly. CC Required for the export of mRNA out of the nucleus, even if the CC mRNA is encoded by an intron-less gene. CC -!- SUBUNIT: Heterodimer with U2AF1. Binds unphosphorylated SF1. CC Interacts with SFRS2IP. CC -!- INTERACTION: CC P54253:ATXN1; NbExp=1; IntAct=EBI-946034, EBI-930964; CC Q01081:U2AF1; NbExp=1; IntAct=EBI-946034, EBI-632461; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- SIMILARITY: Belongs to the splicing factor SR family. CC -!- SIMILARITY: Contains 3 RRM (RNA recognition motif) domains. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X64044; CAA45409.1; -; mRNA. DR PIR; S20250; S20250. DR UniGene; Hs.528007; -. DR PDB; 1JMT; X-ray; B=85-112. DR PDB; 1O0P; NMR; A=372-475. DR PDB; 1OPI; NMR; A=372-475. DR PDB; 1U2F; NMR; A=148-237. DR PDB; 2U2F; NMR; A=258-342. DR DIP; DIP:2154N; -. DR IntAct; P26368; -. DR Ensembl; ENSG00000063244; Homo sapiens. DR KEGG; hsa:11338; -. DR H-InvDB; HIX0015481; -. DR HGNC; HGNC:23156; U2AF2. DR MIM; 191318; gene. DR PharmGKB; PA134908683; -. DR Reactome; REACT_1675.1; mRNA Processing. DR Reactome; REACT_1788.1; Transcription. DR Reactome; REACT_71.1; Gene Expression. DR ArrayExpress; P26368; -. DR GermOnline; ENSG00000063244; Homo sapiens. DR GO; GO:0005681; C:spliceosome; IDA:HGNC. DR GO; GO:0005515; F:protein binding; IPI:UniProtKB. DR GO; GO:0000398; P:nuclear mRNA splicing, via spliceosome; IC:HGNC. DR InterPro; IPR012677; a_b_plait_nuc_bd. DR InterPro; IPR000504; RRM_RNP1. DR InterPro; IPR006529; U2AF_lg. DR Gene3D; G3DSA:3.30.70.330; a_b_plait_nuc_bd; 3. DR Pfam; PF00076; RRM_1; 3. DR SMART; SM00360; RRM; 3. DR TIGRFAMs; TIGR01642; U2AF_lg; 1. DR PROSITE; PS50102; RRM; 3. PE 1: Evidence at protein level; KW 3D-structure; Acetylation; Direct protein sequencing; mRNA processing; KW mRNA splicing; Nucleus; Phosphorylation; Repeat; RNA-binding; KW Spliceosome. FT INIT_MET 1 1 Removed. FT CHAIN 2 475 Splicing factor U2AF 65 kDa subunit. FT /FTId=PRO_0000081988. FT DOMAIN 149 231 RRM 1. FT DOMAIN 259 337 RRM 2. FT DOMAIN 385 466 RRM 3. FT COMPBIAS 27 62 Arg/Ser-rich (RS domain). FT MOD_RES 2 2 N-acetylserine. FT MOD_RES 2 2 Phosphoserine. FT MOD_RES 53 53 Phosphoserine. FT MOD_RES 55 55 Phosphoserine. FT MOD_RES 79 79 Phosphoserine. FT MUTAGEN 92 92 W->A: Decreases affinity for UAF1 by 3 FT orders of magnitude. FT MUTAGEN 96 96 P->G: Decreases affinity for UAF1 by 2 FT orders of magnitude. FT MUTAGEN 104 104 P->G: Decreases affinity for UAF1 by 2 FT orders of magnitude. FT MUTAGEN 387 388 EE->RR: Reduces interaction with SF1. FT MUTAGEN 391 394 DDEE->AAAA: Reduces interaction with SF1. FT MUTAGEN 391 394 DDEE->RRKK: Reduces interaction with SF1. FT MUTAGEN 396 397 EE->AA: No effect. FT MUTAGEN 396 397 EE->GA: Reduces interaction with SF1. FT MUTAGEN 396 397 EE->KK: Reduces interaction with SF1. FT MUTAGEN 454 454 F->A: Reduces interaction with SF1. FT HELIX 104 109 FT STRAND 150 155 FT HELIX 162 175 FT STRAND 180 183 FT STRAND 185 191 FT STRAND 193 204 FT HELIX 205 211 FT HELIX 212 214 FT STRAND 225 227 FT TURN 236 259 FT STRAND 261 264 FT HELIX 272 280 FT STRAND 285 292 FT TURN 294 296 FT STRAND 298 309 FT HELIX 312 320 FT STRAND 331 334 FT STRAND 376 383 FT TURN 386 390 FT HELIX 392 406 FT TURN 407 409 FT STRAND 412 416 FT STRAND 423 425 FT STRAND 431 437 FT HELIX 439 449 FT STRAND 454 457 FT STRAND 460 464 FT HELIX 466 471 SQ SEQUENCE 475 AA; 53501 MW; 26AD271CD8FC6211 CRC64; MSDFDEFERQ LNENKQERDK ENRHRKRSHS RSRSRDRKRR SRSRDRRNRD QRSASRDRRR RSKPLTRGAK EEHGGLIRSP RHEKKKKVRK YWDVPPPGFE HITPMQYKAM QAAGQIPATA LLPTMTPDGL AVTPTPVPVV GSQMTRQARR LYVGNIPFGI TEEAMMDFFN AQMRLGGLTQ APGNPVLAVQ INQDKNFAFL EFRSVDETTQ AMAFDGIIFQ GQSLKIRRPH DYQPLPGMSE NPSVYVPGVV STVVPDSAHK LFIGGLPNYL NDDQVKELLT SFGPLKAFNL VKDSATGLSK GYAFCEYVDI NVTDQAIAGL NGMQLGDKKL LVQRASVGAK NATLVSPPST INQTPVTLQV PGLMSSQVQM GGHPTEVLCL MNMVLPEELL DDEEYEEIVE DVRDECSKYG LVKSIEIPRP VDGVEVPGCG KIFVEFTSVF DCQKAMQGLT GRKFANRVVV TKYCDPDSYH RRDFW //