ID T257_ECOLI STANDARD; PRT; 997 AA. AC P25239; DT 01-MAY-1992 (REL. 22, CREATED) DT 01-MAY-1992 (REL. 22, LAST SEQUENCE UPDATE) DT 01-OCT-1994 (REL. 30, LAST ANNOTATION UPDATE) DE TYPE IIS RESTRICTION ENZYME ECO57I (EC 3.1.21.4) (ENDONUCLEASE DE ECO57I) (CONTAINS: ADENINE-SPECIFIC METHYLTRANSFERASE ACTIVITY DE (EC 2.1.1.72)). GN ECO57IR. OS ESCHERICHIA COLI. OC PROKARYOTA; GRACILICUTES; SCOTOBACTERIA; FACULTATIVELY ANAEROBIC RODS; OC ENTEROBACTERIACEAE. RN [1] RP SEQUENCE FROM N.A., AND PARTIAL SEQUENCE. RC STRAIN=RFL57; RM 93096595 RA JANULAITIS A., VAISVILA R., TIMINSKAS A., KLIMASAUSKAS S., RA BUTKUS V.; RL NUCLEIC ACIDS RES. 20:6051-6056(1992). RN [2] RP CHARACTERIZATION. RM 93096594 RA JANULAITIS A., PETRUSYTE M., MANELIENE Z., KLIMASAUSKAS S., BUTKUS V.; RL NUCLEIC ACIDS RES. 20:6043-6049(1992). CC -!- CATALYTIC ACTIVITY: RECOGNIZES THE DOUBLE-STRANDED SEQUENCES CC CTGAAG AND CTTCAG AND CLEAVES RESPECTIVELY 22 BASES AFTER C-1 CC AND 14 BASES BEFORE C-1. ALSO ACTS AS A METHYLASE THAT CAUSES CC SPECIFIC METHYLATION ON A-5 ON ONE STRAND, THE OTHER STRAND CC BEING METHYLATED BY THE ECO57I METHYLASE. CC -!- CATALYTIC ACTIVITY: S-ADENOSYL-L-METHIONINE + DNA ADENINE = CC S-ADENOSYL-L-HOMOCYSTEINE + DNA 6-METHYLAMINOPURINE. CC -!- SUBUNIT: MONOMER. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M74821; EC571MR. DR EMBL; X61122; ECECO57IM. DR PIR; S26426; S26426. DR REBASE; ECO57I; RELEASE 9410. DR PROSITE; PS00092; N6_MTASE. KW HYDROLASE; ENDONUCLEASE; NUCLEASE; TRANSFERASE; METHYLTRANSFERASE; KW MULTIFUNCTIONAL ENZYME; RESTRICTION SYSTEM. SQ SEQUENCE 997 AA; 116709 MW; 5315720 CN; MNKKDQLQRL IDKYKSDIDY YRSARYNETQ LRTDFLDQLF LLLGWDITNA AGKPTNEREV LVEEGLKARA GENTKKPDYT FRLFSERKFF LEAKKPSVDI STTIEPALQV RRYGFTAKLK ISVLSNFEYT AIYDCSNQVK ETDSVANSRI KLYHFTELVD KFDEINNLIG RESVYTGHFD NEWSEIENKI LRFSVDDLFL KQINDWGLLL ANEFLQIKNE LPEEKLNDLV QNYINSIVFL RVCEDRDLEE YETLYHFAQD KDFQSLVKKL KSSDKKYNSG LFSLEYIDEL LSNANSCIEH HRTIILPHKA HTLSLFSLLI FLGNIYEIFL SEKVRIDELG NVKIQPKEEH IDRDVVTTPT HIVKEIIRNT VVEYCKGKSD IEILNSKFAD IACGSGAFII VAFQFIQDIL IDYYIQNDKS KLQQISEHTY KLKFEVKREI LCKCIYGIDK DYNATKACTF GLLLKLLEGE TTETIGKDTP ILPALDTNIL FGNSLIDSGD KVKQEDIFSI NPFDLTNYQF DVIVGNPPYM ATEHMNQLTP KELDIYKRKY KSAYKQFDKY FLFIERSIQI LKEYGYLGYI LPSRFIKVDA GKKLRKFLSE NKYLSKLISF GSHQVFKNKT TYTCLLFLNK ENHDNFSFYE VKDNKKWLTR EDKYLLSSTY QTSSLDSDTW VLEKKINDIL KLMFSKSEQL GNIVGKSNVA NGIQTSANKY YIHKEIKSEN GFIYFEYDGI EYHIEKELTR PYFETNRSGD DSFYTYKDVE PNSFVVYPYK KVGERIQFIE YDELKRQYPK LFEFLQVVKV HLNDKKRSIK PDPTGLMMVQ IRRSQALENC DVDQKLIVGI LSNGYKYSID NHRTFVSSGG TAGYSIINIP NNVRYSIYYI QAILTSKYLE WFASIYGDIF RGRFVARGTK VQTRMPIPTI DFDDPKQKEI HDTISSKQQY LNKLYSQTQK SADRDKIIFE RQFEQEKIQM DYLIKNLFDL GDLDSEIPTV EDLYKNL //