ID LASR_PSEAE Reviewed; 239 AA. AC P25084; Q51475; Q51476; DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-1992, sequence version 1. DT 11-JAN-2011, entry version 87. DE RecName: Full=Transcriptional activator protein lasR; GN Name=lasR; OrderedLocusNames=PA1430; OS Pseudomonas aeruginosa. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=287; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228; RX MEDLINE=91210193; PubMed=1902216; RA Gambello M.J., Iglewski B.H.; RT "Cloning and characterization of the Pseudomonas aeruginosa lasR gene, RT a transcriptional activator of elastase expression."; RL J. Bacteriol. 173:3000-3009(1991). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 29260 / PA103, and IFO 3455; RX MEDLINE=95023115; PubMed=7937080; DOI=10.1093/nar/22.18.3706; RA Fukushima J., Ishiwata T., Kurata M., You Z., Okuda K.; RT "Intracellular receptor-type transcription factor, LasR, contains a RT highly conserved amphipathic region which precedes the putative helix- RT turn-helix DNA binding motif."; RL Nucleic Acids Res. 22:3706-3707(1994). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228; RX MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079; RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M., RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., RA Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.; RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an RT opportunistic pathogen."; RL Nature 406:959-964(2000). CC -!- FUNCTION: Transcriptional activator of elastase structural gene CC (lasB). Binds to the PAI autoinducer. CC -!- MISCELLANEOUS: LasR in strain PA103 is not active, this is CC probably due to the change in position 180 of the sequence. CC -!- SIMILARITY: Belongs to the autoinducer-regulated transcriptional CC regulatory protein family. CC -!- SIMILARITY: Contains 1 HTH luxR-type DNA-binding domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M59425; AAA25874.1; -; Genomic_DNA. DR EMBL; D30813; BAA06490.1; -; Genomic_DNA. DR EMBL; D30812; BAA06489.1; -; Genomic_DNA. DR EMBL; AE004091; AAG04819.1; -; Genomic_DNA. DR PIR; A43660; A43660. DR PIR; S53692; S53692. DR PIR; S53693; S53693. DR RefSeq; NP_250121.1; NC_002516.2. DR PDB; 2UV0; X-ray; 1.80 A; E/F/G/H=1-173. DR PDB; 3IX3; X-ray; 1.40 A; A/B=1-173. DR PDB; 3IX4; X-ray; 1.80 A; A/B/C/D/E/F/G/H=1-173. DR PDB; 3IX8; X-ray; 1.80 A; A/B/C/D=1-173. DR PDB; 3JPU; X-ray; 2.30 A; A/B/C/D/E=1-173. DR PDBsum; 2UV0; -. DR PDBsum; 3IX3; -. DR PDBsum; 3IX4; -. DR PDBsum; 3IX8; -. DR PDBsum; 3JPU; -. DR ProteinModelPortal; P25084; -. DR SMR; P25084; 2-168. DR GeneID; 881789; -. DR GenomeReviews; AE004091_GR; PA1430. DR KEGG; pae:PA1430; -. DR PseudoCAP; PA1430; -. DR HOGENOM; HBG373201; -. DR OMA; HCATRAT; -. DR ProtClustDB; CLSK866563; -. DR BioCyc; PAER208964:PA1430-MONOMER; -. DR GO; GO:0005622; C:intracellular; IEA:InterPro. DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro. DR GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro. DR GO; GO:0000156; F:two-component response regulator activity; IEA:InterPro. DR GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW. DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro. DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor. DR InterPro; IPR005143; TF_LuxR_autoind-bd_dom. DR InterPro; IPR000792; Tscrpt_reg_LuxR_C. DR InterPro; IPR011991; WHTH_trsnscrt_rep_DNA-bd. DR Gene3D; G3DSA:3.30.450.80; TF_LuxR_autoind-bd_dom; 1. DR Gene3D; G3DSA:1.10.10.10; Wing_hlx_DNA_bd; 1. DR Pfam; PF03472; Autoind_bind; 1. DR Pfam; PF00196; GerE; 1. DR PRINTS; PR00038; HTHLUXR. DR SMART; SM00421; HTH_LUXR; 1. DR SUPFAM; SSF46894; Bipartite_resp_reg_C-effector; 1. DR SUPFAM; SSF75516; TF_LuxR_autoind-bd_dom; 1. DR PROSITE; PS00622; HTH_LUXR_1; 1. DR PROSITE; PS50043; HTH_LUXR_2; 1. PE 1: Evidence at protein level; KW 3D-structure; Activator; Complete proteome; DNA-binding; KW Quorum sensing; Transcription; Transcription regulation. FT CHAIN 1 239 Transcriptional activator protein lasR. FT /FTId=PRO_0000184160. FT DOMAIN 170 235 HTH luxR-type. FT DNA_BIND 194 213 H-T-H motif (By similarity). FT VARIANT 144 144 M -> I (in strain: IFO 3455 and PA103). FT VARIANT 180 180 R -> W (in strain: PA103). FT HELIX 7 12 FT HELIX 15 30 FT STRAND 33 40 FT HELIX 47 49 FT STRAND 51 54 FT HELIX 58 66 FT HELIX 69 71 FT HELIX 74 81 FT HELIX 90 92 FT HELIX 96 107 FT STRAND 111 119 FT STRAND 125 131 FT HELIX 137 167 SQ SEQUENCE 239 AA; 26619 MW; 28B6E06E9187924A CRC64; MALVDGFLEL ERSSGKLEWS AILQKMASDL GFSKILFGLL PKDSQDYENA FIVGNYPAAW REHYDRAGYA RVDPTVSHCT QSVLPIFWEP SIYQTRKQHE FFEEASAAGL VYGLTMPLHG ARGELGALSL SVEAENRAEA NRFMESVLPT LWMLKDYALQ SGAGLAFEHP VSKPVVLTSR EKEVLQWCAI GKTSWEISVI CNCSEANVNF HMGNIRRKFG VTSRRVAAIM AVNLGLITL //