ID LASR_PSEAE STANDARD; PRT; 239 AA. AC P25084; Q51475; Q51476; DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-1992, sequence version 1. DT 07-FEB-2006, entry version 48. DE Transcriptional activator protein lasR. GN Name=lasR; OrderedLocusNames=PA1430; OS Pseudomonas aeruginosa. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=287; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 15692 / PAO1; RX MEDLINE=91210193; PubMed=1902216; RA Gambello M.J., Iglewski B.H.; RT "Cloning and characterization of the Pseudomonas aeruginosa lasR gene, RT a transcriptional activator of elastase expression."; RL J. Bacteriol. 173:3000-3009(1991). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=IFO 3455, and PA103; RX MEDLINE=95023115; PubMed=7937080; RA Fukushima J., Ishiwata T., Kurata M., You Z., Okuda K.; RT "Intracellular receptor-type transcription factor, LasR, contains a RT highly conserved amphipathic region which precedes the putative helix- RT turn-helix DNA binding motif."; RL Nucleic Acids Res. 22:3706-3707(1994). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 15692 / PAO1; RX MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079; RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M., RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., RA Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.; RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an RT opportunistic pathogen."; RL Nature 406:959-964(2000). CC -!- FUNCTION: Transcriptional activator of elastase structural gene CC (lasB). Binds to the PAI autoinducer. CC -!- MISCELLANEOUS: LasR in strain PA103 is not active, this is CC probably due to the change in position 180 of the sequence. CC -!- SIMILARITY: Belongs to the autoinducer-regulated transcriptional CC regulatory protein family. CC -!- SIMILARITY: Contains 1 HTH luxR-type DNA-binding domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M59425; AAA25874.1; -; Genomic_DNA. DR EMBL; D30813; BAA06490.1; -; Genomic_DNA. DR EMBL; D30812; BAA06489.1; -; Genomic_DNA. DR EMBL; AE004572; AAG04819.1; -; Genomic_DNA. DR PIR; A43660; A43660. DR PIR; S53692; S53692. DR HSSP; P11470; 1FSE. DR BioCyc; PAER287:PA1430-MONOMER; -. DR InterPro; IPR005143; Autoind_bd. DR InterPro; IPR000792; HTH_LuxR. DR InterPro; IPR011991; Wing_hlx_DNA_bd. DR Pfam; PF03472; Autoind_bind; 1. DR Pfam; PF00196; GerE; 1. DR PRINTS; PR00038; HTHLUXR. DR ProDom; PD000307; HTH_LuxR; 1. DR SMART; SM00421; HTH_LUXR; 1. DR PROSITE; PS00622; HTH_LUXR_1; 1. DR PROSITE; PS50043; HTH_LUXR_2; 1. KW Activator; Complete proteome; DNA-binding; Quorum sensing; KW Transcription; Transcription regulation. FT CHAIN 1 239 Transcriptional activator protein lasR. FT /FTId=PRO_0000184160. FT DOMAIN 170 235 HTH luxR-type. FT DNA_BIND 194 213 H-T-H motif (By similarity). FT VARIANT 144 144 M -> I (in strain: IFO 3455 and PA103). FT VARIANT 180 180 R -> W (in strain: PA103). SQ SEQUENCE 239 AA; 26619 MW; 28B6E06E9187924A CRC64; MALVDGFLEL ERSSGKLEWS AILQKMASDL GFSKILFGLL PKDSQDYENA FIVGNYPAAW REHYDRAGYA RVDPTVSHCT QSVLPIFWEP SIYQTRKQHE FFEEASAAGL VYGLTMPLHG ARGELGALSL SVEAENRAEA NRFMESVLPT LWMLKDYALQ SGAGLAFEHP VSKPVVLTSR EKEVLQWCAI GKTSWEISVI CNCSEANVNF HMGNIRRKFG VTSRRVAAIM AVNLGLITL //