ID PPBE_MOUSE STANDARD; PRT; 529 AA. AC P24823; DT 01-MAR-1992 (Rel. 21, Created) DT 01-MAR-1992 (Rel. 21, Last sequence update) DT 25-JAN-2005 (Rel. 46, Last annotation update) DE Alkaline phosphatase, embryonic precursor (EC 3.1.3.1) (EAP). GN Name=Akp5; Synonyms=Eap; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE. RX MEDLINE=91139124; PubMed=2286375; RA Manes T., Glade K., Ziomek C.A., Millan J.L.; RT "Genomic structure and comparison of mouse tissue-specific alkaline RT phosphatase genes."; RL Genomics 8:541-554(1990). CC -!- CATALYTIC ACTIVITY: A phosphate monoester + H(2)O = an alcohol + CC phosphate. CC -!- SUBUNIT: Homodimer. CC -!- SUBCELLULAR LOCATION: Attached to the membrane by a GPI-anchor. CC -!- TISSUE SPECIFICITY: Embryo and testis. CC -!- MISCELLANEOUS: In most mammals there are four different isozymes: CC placental, placental-like, intestinal and tissue non-specific CC (liver/bone/kidney). CC -!- SIMILARITY: Belongs to the alkaline phosphatase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M61704; AAA37531.1; -. DR HSSP; P05187; 1EW2. DR Ensembl; ENSMUSG00000026246; Mus musculus. DR MGD; MGI:108009; Akp5. DR GO; GO:0005615; C:extracellular space; TAS. DR InterPro; IPR001952; Alk_phosphtse. DR Pfam; PF00245; Alk_phosphatase; 1. DR PRINTS; PR00113; ALKPHPHTASE. DR SMART; SM00098; alkPPc; 1. DR PROSITE; PS00123; ALKALINE_PHOSPHATASE; 1. KW Glycoprotein; GPI-anchor; Hydrolase; Lipoprotein; Magnesium; KW Multigene family; Phosphorylation; Signal; Transmembrane; Zinc. FT SIGNAL 1 18 Potential. FT CHAIN 19 502 Alkaline phosphatase, embryonic. FT PROPEP 503 529 Removed in mature form (By similarity). FT DISULFID 139 201 By similarity. FT DISULFID 485 492 By similarity. FT LIPID 502 502 GPI-anchor amidated serine (By FT similarity). FT ACT_SITE 110 110 Phosphoserine intermediate. FT CARBOHYD 140 140 N-linked (GlcNAc...) (Potential). FT CARBOHYD 267 267 N-linked (GlcNAc...) (Potential). FT CARBOHYD 277 277 N-linked (GlcNAc...) (Potential). SQ SEQUENCE 529 AA; 57212 MW; 2E0CD655AD4B30BA CRC64; MWGACLLLLG LSLQVCPSVI PVEEENPAFW NRKAAEALDA AKKLKPIQTS AKNLVILMGD GMGVSTVTAT RILKGQQQGL SGPETQLAMD RFPHMALSKT YNTDKQIPDS AGTGTAFLCG VKTNMKVIGL SAAARFNQCN TTWGNEVVSV MHRAKKAGKS VGVVTTTSVQ HASPAGTYAH TVNRGWYSDA QMPASALQDG CKDISTQLIS NMDIDVILGG GRKFMFPKGT PDQEYPTDTK QAGTRLDGRN LVQEWLAKHQ GARYVWNRSE LIQASLNRSV THLMGLFEPN DMKYEIHRDP AQDPSLAEMT EVAVRMLSRN PKGFYLFVEG GRIDHGHHET VAYRALTEAV MFDSAVDKAD IRTSEQDTMI LVTADHSHVF SFGGYTQRGA SIFGLAPFKA EDGKSFTSIL YGNGPGYKLH NGARADVTEE ESSNPTYQQQ ACVPLSSETH SGEDVAIFAR GPQAHLVHGV QEQNYIAHVM AFAACLEPYT DCGLASPAGQ SSAVSPGYMS TLLCLLAGKM LMLMAAAEP //