ID PPBE_MOUSE STANDARD; PRT; 529 AA. AC P24823; DT 01-MAR-1992 (REL. 21, CREATED) DT 01-MAR-1992 (REL. 21, LAST SEQUENCE UPDATE) DT 01-MAY-1992 (REL. 22, LAST ANNOTATION UPDATE) DE ALKALINE PHOSPHATASE, EMBRYONIC PRECURSOR (EC 3.1.3.1) (EAP). GN EAP. OS MUS MUSCULUS (MOUSE). OC EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; TETRAPODA; MAMMALIA; OC EUTHERIA; RODENTIA. RN [1] RP SEQUENCE FROM N.A. RM 91139124 RA MANES T., GLADE K., ZIOMEK C.A., MILLAN J.L.; RL GENOMICS 8:541-554(1990). CC -!- CATALYTIC ACTIVITY: AN ORTHOPHOSPHORIC MONOESTER + H(2)O = AN CC ALCOHOL + ORTHOPHOSPHATE (AT A HIGH PH OPTIMUM). CC -!- SUBUNIT: HOMODIMER. CC -!- TISSUE SPECIFICITY: EMBRYO AND TESTIS. CC -!- SUBCELLULAR LOCATION: ATTACHED TO THE MEMBRANE BY A GPI-ANCHOR. CC -!- SIMILARITY: STRONG TO ALL OTHER MAMMALIAN AP, AND TO BACTERIAL CC AND YEAST AP. CC -!- IN MOST MAMMALS THERE ARE FOUR DIFFERENT ISOZYMES: PLACENTAL, CC PLACENTAL-LIKE, INTESTINAL AND TISSUE NON-SPECIFIC (LIVER/BONE/ CC KIDNEY). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M61704; MMEAP. DR PIR; A36307; A36307. DR HSSP; P00634; 1ALK. DR PROSITE; PS00123; ALKALINE_PHOSPHATASE. KW HYDROLASE; ZINC; MAGNESIUM; PHOSPHORYLATION; TRANSMEMBRANE; KW MULTIGENE FAMILY; GLYCOPROTEIN; GPI-ANCHOR; SIGNAL. FT SIGNAL 1 18 POTENTIAL. FT CHAIN 19 502 ALKALINE PHOSPHATASE, EMBRYONIC. FT PROPEP 503 529 REMOVED IN MATURE FORM (BY SIMILARITY). FT LIPID 502 502 GPI-ANCHOR (BY SIMILARITY). FT MOD_RES 110 110 PHOSPHORYLATION. FT ACT_SITE 110 110 FT CARBOHYD 140 140 POTENTIAL. FT CARBOHYD 267 267 POTENTIAL. FT CARBOHYD 277 277 POTENTIAL. SQ SEQUENCE 529 AA; 57212 MW; 1408458 CN; MWGACLLLLG LSLQVCPSVI PVEEENPAFW NRKAAEALDA AKKLKPIQTS AKNLVILMGD GMGVSTVTAT RILKGQQQGL SGPETQLAMD RFPHMALSKT YNTDKQIPDS AGTGTAFLCG VKTNMKVIGL SAAARFNQCN TTWGNEVVSV MHRAKKAGKS VGVVTTTSVQ HASPAGTYAH TVNRGWYSDA QMPASALQDG CKDISTQLIS NMDIDVILGG GRKFMFPKGT PDQEYPTDTK QAGTRLDGRN LVQEWLAKHQ GARYVWNRSE LIQASLNRSV THLMGLFEPN DMKYEIHRDP AQDPSLAEMT EVAVRMLSRN PKGFYLFVEG GRIDHGHHET VAYRALTEAV MFDSAVDKAD IRTSEQDTMI LVTADHSHVF SFGGYTQRGA SIFGLAPFKA EDGKSFTSIL YGNGPGYKLH NGARADVTEE ESSNPTYQQQ ACVPLSSETH SGEDVAIFAR GPQAHLVHGV QEQNYIAHVM AFAACLEPYT DCGLASPAGQ SSAVSPGYMS TLLCLLAGKM LMLMAAAEP //