ID DGAL_CITFR Reviewed; 332 AA. AC P23925; DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-1992, sequence version 1. DT 10-FEB-2021, entry version 85. DE RecName: Full=D-galactose-binding periplasmic protein; DE Short=GBP; DE AltName: Full=D-galactose/ D-glucose-binding protein; DE Short=GGBP; DE Flags: Precursor; GN Name=mglB; OS Citrobacter freundii. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Citrobacter; Citrobacter freundii complex. OX NCBI_TaxID=546; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 8090 / DSM 30039 / JCM 1657 / LMG 3246 / NCTC 9750; RA Galindo R.L., Daggett Garvin L., Hardies S.C.; RL Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: This protein is involved in the active transport of galactose CC and glucose. It plays a role in the chemotaxis towards the two sugars CC by interacting with the trg chemoreceptor. CC -!- SUBCELLULAR LOCATION: Periplasm. CC -!- DOMAIN: The calcium-binding site is structurally similar to that of EF- CC hand proteins, but is in two parts, with the last calcium ligand CC provided by Glu-228. CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 2 family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X59389; CAA42032.1; -; Genomic_DNA. DR PIR; S15554; S15554. DR RefSeq; WP_003027399.1; NZ_VTEC01000008.1. DR SMR; P23925; -. DR PRIDE; P23925; -. DR GeneID; 58184983; -. DR OrthoDB; 1378040at2; -. DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell. DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW. DR GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-KW. DR CDD; cd01539; PBP1_GGBP; 1. DR InterPro; IPR044085; MglB-like_PBP1. DR InterPro; IPR028082; Peripla_BP_I. DR InterPro; IPR025997; SBP_2_dom. DR Pfam; PF13407; Peripla_BP_4; 1. DR SUPFAM; SSF53822; SSF53822; 1. PE 3: Inferred from homology; KW Calcium; Chemotaxis; Metal-binding; Periplasm; Signal; Sugar transport; KW Transport. FT SIGNAL 1..23 FT CHAIN 24..332 FT /note="D-galactose-binding periplasmic protein" FT /id="PRO_0000031721" FT CA_BIND 157..165 FT /note="First part of site" FT CA_BIND 227..228 FT /note="Second part of site" FT SITE 97 FT /note="Interacts with membrane-bound trg signal transducer" SQ SEQUENCE 332 AA; 35817 MW; 2530E7EDE0471B1B CRC64; MNKKVLTLSA VMASMLFGAA AHAADTRIGV TIYKYDDNFM SVVRKAIEKD AKAAPDVQLL MNDSQNDQSK QNDQIDVLLA KGVKALAINL VDPAAAGTVI EKARGQNVPI VFFNKEPSRK ALDSYDKAYY VGTDSKESGI IQGDLIAKHW AANPNWDLNK DGKIQFVLLK GEPGHPDAEA RTTYVIKELN DKGIKTEQLQ LDTAMWDTAQ AKDKMDAWMS GPNANKIEVV IANNDAMAMG AVEALKAHNK TSVPVFGVDA LPEALALVKS GAMAGTVLND ANNQAKATFD LAKNLADGKG AADGTNWKIE NKIVRVPYVG VDKDNLAEFT NK //