ID DGAL_CITFR Reviewed; 332 AA. AC P23925; DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-1992, sequence version 1. DT 24-NOV-2009, entry version 52. DE RecName: Full=D-galactose-binding periplasmic protein; DE Short=GBP; DE AltName: Full=D-galactose/ D-glucose-binding protein; DE Short=GGBP; DE Flags: Precursor; GN Name=mglB; OS Citrobacter freundii. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Citrobacter. OX NCBI_TaxID=546; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 8090 / DSM 30039 / JCM 1657 / LMG 3246 / NCTC 9750; RA Galindo R.L., Daggett Garvin L., Hardies S.C.; RL Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: This protein is involved in the active transport of CC galactose and glucose. It plays a role in the chemotaxis towards CC the two sugars by interacting with the trg chemoreceptor. CC -!- SUBCELLULAR LOCATION: Periplasm. CC -!- DOMAIN: The calcium-binding site is structurally similar to that CC of EF-hand proteins, but is in two parts, with the last calcium CC ligand provided by Glu-228. CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 2 CC family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X59389; CAA42032.1; -; Genomic_DNA. DR PIR; S15554; S15554. DR SMR; P23925; 25-329. DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell. DR GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-KW. DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW. DR GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-KW. DR InterPro; IPR001761; Peripla_BP_Lac1. DR Pfam; PF00532; Peripla_BP_1; 1. PE 3: Inferred from homology; KW Calcium; Chemotaxis; Periplasm; Signal; Sugar transport; Transport. FT SIGNAL 1 23 FT CHAIN 24 332 D-galactose-binding periplasmic protein. FT /FTId=PRO_0000031721. FT CA_BIND 157 165 First part of site. FT CA_BIND 227 228 Second part of site. FT SITE 97 97 Interacts with membrane-bound trg signal FT transducer. SQ SEQUENCE 332 AA; 35817 MW; 2530E7EDE0471B1B CRC64; MNKKVLTLSA VMASMLFGAA AHAADTRIGV TIYKYDDNFM SVVRKAIEKD AKAAPDVQLL MNDSQNDQSK QNDQIDVLLA KGVKALAINL VDPAAAGTVI EKARGQNVPI VFFNKEPSRK ALDSYDKAYY VGTDSKESGI IQGDLIAKHW AANPNWDLNK DGKIQFVLLK GEPGHPDAEA RTTYVIKELN DKGIKTEQLQ LDTAMWDTAQ AKDKMDAWMS GPNANKIEVV IANNDAMAMG AVEALKAHNK TSVPVFGVDA LPEALALVKS GAMAGTVLND ANNQAKATFD LAKNLADGKG AADGTNWKIE NKIVRVPYVG VDKDNLAEFT NK //