ID DGAL_CITFR STANDARD; PRT; 332 AA. AC P23925; DT 01-MAR-1992 (REL. 21, CREATED) DT 01-MAR-1992 (REL. 21, LAST SEQUENCE UPDATE) DT 01-MAY-1992 (REL. 22, LAST ANNOTATION UPDATE) DE D-GALACTOSE-BINDING PROTEIN PRECURSOR (GBP). GN MGLB. OS CITROBACTER FREUNDII. OC PROKARYOTA; GRACILICUTES; SCOTOBACTERIA; FACULTATIVELY ANAEROBIC RODS; OC ENTEROBACTERIACEAE. RN [1] RP SEQUENCE FROM N.A. RC STRAIN=ATCC 8090; RA GALINDO R.L., DAGGET GARVIN L., HARDIES S.C.; RL SUBMITTED (MAY-1991) TO EMBL/GENBANK/DDBJ DATA BANKS. CC -!- FUNCTION: THIS PROTEIN IS INVOLVED IN THE ACTIVE TRANSPORT OF CC GALACTOSE. IT PLAYS A ROLE IN THE CHEMOTAXIS TOWARD GALACTOSE. CC -!- SUBCELLULAR LOCATION: PERIPLASMIC SPACE. CC -!- THE CALCIUM-BINDING SITE IS STRUCTURALLY SIMILAR TO THAT OF CC EF-HAND PROTEINS, BUT IS IN TWO PARTS, WITH THE LAST CALCIUM CC LIGAND PROVIDED BY GLU-228. CC -!- SIMILARITY: TO THE E.COLI D-RIBOSE-BINDING PERIPLASMIC (RBP) AND CC THE A.TUMEFACIENS PUTATIVE PERIPLASMIC RECEPTOR PROTEIN CHVE. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X59389; CFMGLB. DR PIR; S15554; S15554. KW TRANSPORT; SUGAR TRANSPORT; PERIPLASMIC; CALCIUM-BINDING; CHEMOTAXIS; KW SIGNAL. FT SIGNAL 1 23 FT CHAIN 24 332 D-GALACTOSE-BINDING PROTEIN. FT CA_BIND 157 165 FIRST PART OF SITE. FT CA_BIND 227 228 SECOND PART OF SITE. FT SITE 97 97 INTERACT WITH MEMBRANE-BOUND TRG SIGNAL FT TRANSDUCER. SQ SEQUENCE 332 AA; 35817 MW; 511666 CN; MNKKVLTLSA VMASMLFGAA AHAADTRIGV TIYKYDDNFM SVVRKAIEKD AKAAPDVQLL MNDSQNDQSK QNDQIDVLLA KGVKALAINL VDPAAAGTVI EKARGQNVPI VFFNKEPSRK ALDSYDKAYY VGTDSKESGI IQGDLIAKHW AANPNWDLNK DGKIQFVLLK GEPGHPDAEA RTTYVIKELN DKGIKTEQLQ LDTAMWDTAQ AKDKMDAWMS GPNANKIEVV IANNDAMAMG AVEALKAHNK TSVPVFGVDA LPEALALVKS GAMAGTVLND ANNQAKATFD LAKNLADGKG AADGTNWKIE NKIVRVPYVG VDKDNLAEFT NK //