ID DGAL_CITFR STANDARD; PRT; 332 AA. AC P23925; DT 01-MAR-1992 (Rel. 21, Created) DT 01-MAR-1992 (Rel. 21, Last sequence update) DT 29-MAR-2004 (Rel. 43, Last annotation update) DE D-galactose-binding periplasmic protein precursor (GBP) (D-galactose/ DE D-glucose binding protein) (GGBP). GN MGLB. OS Citrobacter freundii. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Citrobacter. OX NCBI_TaxID=546; RN [1] RP SEQUENCE FROM N.A. RC STRAIN=ATCC 8090; RA Galindo R.L., Daggett Garvin L., Hardies S.C.; RL Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: This protein is involved in the active transport of CC galactose and glucose. It plays a role in the chemotaxis towards CC the two sugars by interacting with the trg chemoreceptor. CC -!- SUBCELLULAR LOCATION: Periplasmic. CC -!- DOMAIN: The calcium-binding site is structurally similar to CC that of EF-hand proteins, but is in two parts, with the last CC calcium ligand provided by Glu-228. CC -!- SIMILARITY: Belongs to the bacterial extracellular solute-binding CC protein family 2. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X59389; CAA42032.1; -. DR PIR; S15554; S15554. DR HSSP; P02927; 2GBP. DR InterPro; IPR001761; PeriplaBP/Lac1. DR Pfam; PF00532; Peripla_BP_1; 1. KW Transport; Sugar transport; Periplasmic; Calcium-binding; Chemotaxis; KW Signal. FT SIGNAL 1 23 FT CHAIN 24 332 D-galactose-binding periplasmic protein. FT CA_BIND 157 165 First part of site. FT CA_BIND 227 228 Second part of site. FT SITE 97 97 INTERACT WITH MEMBRANE-BOUND TRG SIGNAL FT TRANSDUCER. SQ SEQUENCE 332 AA; 35817 MW; 2530E7EDE0471B1B CRC64; MNKKVLTLSA VMASMLFGAA AHAADTRIGV TIYKYDDNFM SVVRKAIEKD AKAAPDVQLL MNDSQNDQSK QNDQIDVLLA KGVKALAINL VDPAAAGTVI EKARGQNVPI VFFNKEPSRK ALDSYDKAYY VGTDSKESGI IQGDLIAKHW AANPNWDLNK DGKIQFVLLK GEPGHPDAEA RTTYVIKELN DKGIKTEQLQ LDTAMWDTAQ AKDKMDAWMS GPNANKIEVV IANNDAMAMG AVEALKAHNK TSVPVFGVDA LPEALALVKS GAMAGTVLND ANNQAKATFD LAKNLADGKG AADGTNWKIE NKIVRVPYVG VDKDNLAEFT NK //