ID   INTR_SACER              Reviewed;         437 AA.
AC   P22877;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   12-AUG-2020, entry version 77.
DE   RecName: Full=Integrase;
DE   AltName: Full=Recombinase;
GN   Name=int;
OS   Saccharopolyspora erythraea (Streptomyces erythraeus).
OG   Plasmid pSE211.
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Saccharopolyspora.
OX   NCBI_TaxID=1836;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ER720;
RX   PubMed=2180909; DOI=10.1128/jb.172.4.1877-1888.1990;
RA   Brown D.P., Idler K.B., Katz L.;
RT   "Characterization of the genetic elements required for site-specific
RT   integration of plasmid pSE211 in Saccharopolyspora erythraea.";
RL   J. Bacteriol. 172:1877-1888(1990).
CC   -!- FUNCTION: Is a recombinase (or integrase), catalyzing the cutting and
CC       rejoining of the recombining DNA molecules.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; M35138; AAA98345.1; -; Genomic_DNA.
DR   PIR; C35147; C35147.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   Pfam; PF14659; Phage_int_SAM_3; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..437
FT                   /note="Integrase"
FT                   /id="PRO_0000197534"
FT   DOMAIN          73..158
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          179..428
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        214
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        245
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        379
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        382
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        405
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        414
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   437 AA;  50324 MW;  29EED7108CC5AFBD CRC64;
     MEQKRTRNPN GRSTIYLGND GYWHGRVTMG IGDDGKPDRR HVKRKDKDEV VEEVGKLERE
     RDSGNVRKKG QPWTVERWLT HWVESIAPLT CRYKTMRGYQ TAVYKHLIPG LGAHRLDRIQ
     NHPEYFEKFY LRMIESGLKP ATAHQVHRTA RTAFGEAYKR GRIQRNPVSI AKAPRVEEEE
     VEPLEVEDMQ LVIKAALERR NGVRYVIALA LGTRQGESLA LKWPRLNRQK RTLRITKALQ
     RQTWKHGCSD PHRCGATYHK TEPCKAACKR HTRACPPPCP PACTEHARWC PQRTGGGLVE
     VDVKSRAGRR TVTLPDQLFD LILKHEKLQG AERELAGTEW HDGEWMFTQP NGKPIDPRQD
     LDEWKAILVE AGVREARLHD ARHTAATVLL VLGVPDRVVM ELMGWSSVTM KQRYMHVIDS
     VRNDVADRLN TYFWGTN
//