ID CHL1_YEAST STANDARD; PRT; 861 AA. AC P22516; DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1991, sequence version 1. DT 17-OCT-2006, entry version 58. DE Probable ATP-dependent RNA helicase CHL1 (EC 3.6.1.-). GN Name=CHL1; Synonyms=CTF1; OrderedLocusNames=YPL008W; GN ORFNames=YP8132.05; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=91092258; PubMed=2265610; RA Gerring S.L., Spencer F., Hieter P.; RT "The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is RT important for chromosome transmission and normal cell cycle RT progression in G2/M."; RL EMBO J. 9:4347-4358(1990). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204511 / S288c / AB972; RX MEDLINE=97313271; PubMed=9169875; RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., RA Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., RA Chung E., Churcher C.M., Coster F., Davis K., Davis R.W., RA Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., RA Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., RA Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., RA Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., RA Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., RA Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., RA Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., RA Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., RA Zollner A., Vo D.H., Hani J.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."; RL Nature 387:103-105(1997). RN [3] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [4] RP INTERACTION WITH ECO1. RX PubMed=15020404; DOI=10.1534/genetics.166.1.33; RA Skibbens R.V.; RT "Chl1p, a DNA helicase-like protein in budding yeast, functions in RT sister-chromatid cohesion."; RL Genetics 166:33-42(2004). CC -!- FUNCTION: Important for chromosome transmission and normal cell CC cycle progression in G(2)/M. Could be an ATP-dependent DNA-binding CC helicase. CC -!- SUBUNIT: Interacts with ECO1. CC -!- INTERACTION: CC P43605:ECO1; NbExp=2; IntAct=EBI-4600, EBI-22988; CC -!- MISCELLANEOUS: Present with 98 molecules/cell. CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH CC subfamily. DDX11/CHL1 sub-subfamily. CC -!- SIMILARITY: Contains 1 helicase ATP-binding domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U33335; AAB68097.1; -; Genomic_DNA. DR EMBL; X56584; CAA39922.1; -; Genomic_DNA. DR EMBL; Z48483; CAA88378.1; -; Genomic_DNA. DR EMBL; Z71255; CAA95033.1; -; Genomic_DNA. DR PIR; S12499; S12499. DR IntAct; P22516; -. DR GermOnline; 143990; -. DR Ensembl; YPL008W; Saccharomyces cerevisiae. DR GenomeReviews; U00094_GR; YPL008W. DR SGD; S000005929; CHL1. DR BioCyc; SCER-S28-01:SCER-S28-01-005863-MONOMER; -. DR LinkHub; P22516; -. DR GO; GO:0005634; C:nucleus; IDA. DR GO; GO:0003678; F:DNA helicase activity; IMP. DR GO; GO:0007059; P:chromosome segregation; IDA. DR GO; GO:0007064; P:mitotic sister chromatid cohesion; IMP. DR InterPro; IPR010614; DEAD_2. DR InterPro; IPR002464; DEAH_box. DR InterPro; IPR006554; DEXDc2. DR InterPro; IPR006555; Helic_c2. DR InterPro; IPR014013; Helic_SF1/SF2_ATP_bd_DinG/Rad3. DR InterPro; IPR013020; Rad3. DR Pfam; PF06733; DEAD_2; 1. DR SMART; SM00488; DEXDc2; 1. DR SMART; SM00491; HELICc2; 1. DR TIGRFAMs; TIGR00604; rad3; 1. DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1. DR PROSITE; PS51193; HELICASE_ATP_BIND_2; 1. KW ATP-binding; Cell cycle; Complete proteome; DNA-binding; Helicase; KW Hydrolase; Nucleotide-binding. FT CHAIN 1 861 Probable ATP-dependent RNA helicase CHL1. FT /FTId=PRO_0000055138. FT DOMAIN 6 458 Helicase ATP-binding. FT NP_BIND 42 49 ATP (By similarity). FT MOTIF 393 396 DEAH box. SQ SEQUENCE 861 AA; 98806 MW; 15D0CC397A77F8F2 CRC64; MDKKEYSETF YHPYKPYDIQ VQLMETVYRV LSEGKKIAIL ESPTGTGKTL SLICATMTWL RMNKADIFTR METNIKTNED DSENLSDDEP DWVIDTYRKS VLQEKVDLLN DYEKHLNEIN TTSCKQLKTM CDLDKEHGRY KSVDPLRKKR KGARHLDVSL EEQDFIPRPY ESDSENNDTS KSTRGGRISD KDYKLSELNS QIITLLDKID GKVSRDPNNG DRFDVTNQNP VKIYYASRTY SQLGQFTSQL RLPSFPSSFR DKVPDEKVKY LPLASKKQLC INPKVMKWKT LEAINDACAD LRHSKEGCIF YQNTNEWRHC PDTLALRDMI FSEIQDIEDL VPLGKSLGIC PYYASREALP IAEVVTLPYQ YLLSESTRSS LQINLENSIV IIDEAHNLIE TINSIYSSQI SLEDLKNCHK GIVTYFNKFK SRLNPGNRVN LLKLNSLLMT LIQFIVKNFK KIGQEIDPND MFTGSNIDTL NIHKLLRYIK VSKIAYKIDT YNQALKEEES SKNENPIKET HKKSVSSQPL LFKVSQFLYC LTNLTSEGQF FFEKNYSIKY MLLEPSKPFE SILNQAKCVV LAGGTMEPMS EFLSNLLPEV PSEDITTLSC NHVIPKENLQ TYITNQPELE FTFEKRMSPS LVNNHLFQFF VDLSKAVPKK GGIVAFFPSY QYLAHVIQCW KQNDRFATLN NVRKIFYEAK DGDDILSGYS DSVAEGRGSL LLAIVGGKLS EGINFQDDLC RAVVMVGLPF PNIFSGELIV KRKHLAAKIM KSGGTEEEAS RATKEFMENI CMKAVNQSVG RAIRHANDYA NIYLLDVRYN RPNFRKKLSR WVQDSINSEH TTHQVISSTR KFFSMRSLNS R //