ID KPYK_EMENI STANDARD; PRT; 526 AA. AC P22360; DT 01-AUG-1991 (Rel. 19, Created) DT 01-AUG-1991 (Rel. 19, Last sequence update) DT 15-JUN-2004 (Rel. 44, Last annotation update) DE Pyruvate kinase (EC 2.7.1.40) (PK). GN PKIA OR PKI. OS Emericella nidulans (Aspergillus nidulans). OC Eukaryota; Fungi; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiales; Trichocomaceae; Emericella. OX NCBI_TaxID=162425; RN [1] RP SEQUENCE FROM N.A. RC STRAIN=WG096; RX MEDLINE=89208879; PubMed=3072099; RA de Graaff L., Visser J.; RT "Structure of the Aspergillus nidulans pyruvate kinase gene."; RL Curr. Genet. 14:553-560(1988). CC -!- CATALYTIC ACTIVITY: ATP + pyruvate = ADP + phosphoenolpyruvate. CC -!- COFACTOR: Requires magnesium and potassium. CC -!- ENZYME REGULATION: Regulated by phosphoenolpyruvate substrate and CC allosteric effectors such as fructose 1,6 diphosphate and CC magnesium. CC -!- PATHWAY: Glycolysis; final step. CC -!- SUBUNIT: Homotetramer. CC -!- SIMILARITY: Belongs to the pyruvate kinase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M36918; AAA33320.1; -. DR PIR; S27364; S27364. DR HSSP; P14178; 1E0T. DR InterPro; IPR001697; Pyruvate_kinase. DR Pfam; PF00224; PK; 1. DR Pfam; PF02887; PK_C; 1. DR PRINTS; PR01050; PYRUVTKNASE. DR ProDom; PD001009; Pyruvate_kinase; 1. DR TIGRFAMs; TIGR01064; pyruv_kin; 1. DR PROSITE; PS00110; PYRUVATE_KINASE; 1. KW Transferase; Kinase; Allosteric enzyme; Glycolysis; Magnesium; KW Phosphorylation. FT MOD_RES 36 36 Phosphoserine (Potential). FT ACT_SITE 254 254 By similarity. FT METAL 256 256 Magnesium (Potential). FT METAL 277 277 Magnesium (Potential). FT METAL 278 278 Magnesium (Potential). FT BINDING 351 351 ADP (Potential). SQ SEQUENCE 526 AA; 58075 MW; 4E3D474B0B062FCF CRC64; MAASSSLDHL SNRMKLEWHS KLNTEMVPAK NFRRTSIICT IGPKTNSVEK INALRRAGLN VVRMNFSHGS YEYHQSVIDH AREAEKQAAG RPVAIALDTK GPEIRTGNTV GDKDIPIKAG HEMNISTDEQ YATASDDQNM YVDYKNITKV ISAGKLIYVD DGILSFEVLE VVDDKTLRVR CLNNGNISSR KGVNLPGTDV DLPALSEKDI SDLKFGVKNK VDMVFASFIR RGSDIRHIRE VLGEEGREIQ IIAKIENQQG VNNFDEILEE TDGVMVARGD LGIEIPAPKV FIAQKMMIAK CNIKGKPVIC ATQMLESMTY NPRPTRAEVS DVANAVLDGA DCVMLSGETA KGNYPCEAVT MMSETCLLAE VAIPHFNVFD ELRNLAPRPT DTVESIAMAA VSASLELNAG AIVVLTTSGN TARMISKYRP VCPIIMVSRN PAATRYSHLY RGVWPFYFPE KKPDFNVKIW QEDVDRRLKW GINHGLKLGI INKGDNIVCV QGWRGGMGHT NTVRVVPAEE NLGLSE //