ID KPYK_ASPNI STANDARD; PRT; 526 AA. AC P22360; DT 01-AUG-1991 (REL. 19, CREATED) DT 01-AUG-1991 (REL. 19, LAST SEQUENCE UPDATE) DT 01-NOV-1991 (REL. 20, LAST ANNOTATION UPDATE) DE PYRUVATE KINASE (EC 2.7.1.40). GN PKI. OS ASPERGILLUS NIDULANS. OC EUKARYOTA; FUNGI; ASCOMYCOTINA; PLECTOMYCETES; EUROTIALES. RN [1] RP SEQUENCE FROM N.A. RC STRAIN=WG096; RM 89208879 RA DE GRAAFF L., VISSER J.; RL CURR. GENET. 14:553-560(1988). CC -!- CATALYTIC ACTIVITY: ATP + PYRUVATE = ADP + PHOSPHOENOLPYRUVATE. CC -!- SUBUNIT: HOMOTETRAMER. CC -!- COFACTOR: REQUIRES MAGNESIUM AND POTASSIUM. CC -!- PATHWAY: FINAL STEP IN GLYCOLYSIS. CC -!- ENZYME REGULATION: REGULATED BY PHOSPHOENOLPYRUVATE SUBSTRATE AND CC ALLOSTERIC EFFECTORS SUCH AS FRUCTOSE 1,6 DIPHOSPHATE AND CC MAGNESIUM. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M36918; ANPKI. DR PROSITE; PS00110; PYRUVATE_KINASE. KW TRANSFERASE; KINASE; GLYCOLYSIS; MAGNESIUM; PHOSPHORYLATION. FT MOD_RES 36 36 PHOSPHORYLATION (POTENTIAL). FT ACT_SITE 254 254 BY SIMILARITY. FT METAL 256 256 MAGNESIUM (POTENTIAL). FT METAL 277 277 MAGNESIUM (POTENTIAL). FT METAL 278 278 MAGNESIUM (POTENTIAL). FT BINDING 351 351 ADP (POTENTIAL). SQ SEQUENCE 526 AA; 58075 MW; 1396615 CN; MAASSSLDHL SNRMKLEWHS KLNTEMVPAK NFRRTSIICT IGPKTNSVEK INALRRAGLN VVRMNFSHGS YEYHQSVIDH AREAEKQAAG RPVAIALDTK GPEIRTGNTV GDKDIPIKAG HEMNISTDEQ YATASDDQNM YVDYKNITKV ISAGKLIYVD DGILSFEVLE VVDDKTLRVR CLNNGNISSR KGVNLPGTDV DLPALSEKDI SDLKFGVKNK VDMVFASFIR RGSDIRHIRE VLGEEGREIQ IIAKIENQQG VNNFDEILEE TDGVMVARGD LGIEIPAPKV FIAQKMMIAK CNIKGKPVIC ATQMLESMTY NPRPTRAEVS DVANAVLDGA DCVMLSGETA KGNYPCEAVT MMSETCLLAE VAIPHFNVFD ELRNLAPRPT DTVESIAMAA VSASLELNAG AIVVLTTSGN TARMISKYRP VCPIIMVSRN PAATRYSHLY RGVWPFYFPE KKPDFNVKIW QEDVDRRLKW GINHGLKLGI INKGDNIVCV QGWRGGMGHT NTVRVVPAEE NLGLSE //