ID   HSP74_YEAST             Reviewed;         642 AA.
AC   P22202; D3DM10;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   29-MAY-2013, entry version 125.
DE   RecName: Full=Heat shock protein SSA4;
GN   Name=SSA4; OrderedLocusNames=YER103W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DS10;
RX   PubMed=2121731;
RA   Boorstein W.R., Craig E.A.;
RT   "Structure and regulation of the SSA4 HSP70 gene of Saccharomyces
RT   cerevisiae.";
RL   J. Biol. Chem. 265:18912-18921(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   PubMed=9169868;
RA   Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA   Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G.,
RA   Hunicke-Smith S., Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H.,
RA   Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P.,
RA   Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T.,
RA   Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL   Nature 387:78-81(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RG   Saccharomyces Genome Database;
RL   Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423; THR-493 AND
RP   SER-552, AND MASS SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- INDUCTION: HSP SSA4 expression is restricted to conditions of
CC       stress.
CC   -!- MISCELLANEOUS: Present with 17900 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
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DR   EMBL; J05637; AAA63574.1; -; Genomic_DNA.
DR   EMBL; U18839; AAB64658.1; -; Genomic_DNA.
DR   EMBL; BK006939; DAA07764.1; -; Genomic_DNA.
DR   PIR; B36590; B36590.
DR   RefSeq; NP_011029.3; NM_001178994.3.
DR   PDB; 3UPV; X-ray; 1.60 A; B=636-642.
DR   PDBsum; 3UPV; -.
DR   ProteinModelPortal; P22202; -.
DR   SMR; P22202; 1-605.
DR   DIP; DIP-3916N; -.
DR   IntAct; P22202; 13.
DR   MINT; MINT-523139; -.
DR   STRING; 4932.YER103W; -.
DR   PaxDb; P22202; -.
DR   PeptideAtlas; P22202; -.
DR   PRIDE; P22202; -.
DR   EnsemblFungi; YER103W; YER103W; YER103W.
DR   GeneID; 856840; -.
DR   KEGG; sce:YER103W; -.
DR   SGD; S000000905; SSA4.
DR   eggNOG; COG0443; -.
DR   GeneTree; ENSGT00700000104788; -.
DR   HOGENOM; HOG000228135; -.
DR   KO; K03283; -.
DR   OMA; DVANPIM; -.
DR   OrthoDB; EOG476P7G; -.
DR   BioCyc; YEAST:G3O-30268-MONOMER; -.
DR   NextBio; 983153; -.
DR   Genevestigator; P22202; -.
DR   GermOnline; YER103W; Saccharomyces cerevisiae.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATPase activity; ISS:SGD.
DR   GO; GO:0051082; F:unfolded protein binding; IGI:SGD.
DR   GO; GO:0006457; P:protein folding; IGI:SGD.
DR   GO; GO:0006950; P:response to stress; IDA:SGD.
DR   GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IMP:SGD.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   Pfam; PF00012; HSP70; 1.
DR   PRINTS; PR00301; HEATSHOCK70.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm;
KW   Nucleotide-binding; Phosphoprotein; Reference proteome;
KW   Stress response.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    642       Heat shock protein SSA4.
FT                                /FTId=PRO_0000078388.
FT   MOD_RES       2      2       N-acetylserine (By similarity).
FT   MOD_RES     423    423       Phosphoserine.
FT   MOD_RES     493    493       Phosphothreonine.
FT   MOD_RES     552    552       Phosphoserine.
FT   HELIX       637    639
SQ   SEQUENCE   642 AA;  69651 MW;  7A976357AC35C352 CRC64;
     MSKAVGIDLG TTYSCVAHFA NDRVEIIAND QGNRTTPSYV AFTDTERLIG DAAKNQAAMN
     PHNTVFDAKR LIGRKFDDPE VTNDAKHYPF KVIDKGGKPV VQVEYKGETK TFTPEEISSM
     ILTKMKETAE NFLGTEVKDA VVTVPAYFND SQRQATKDAG TIAGLNVLRI INEPTAAAIA
     YGLDKKSQKE HNVLIFDLGG GTFDVSLLSI DEGVFEVKAT AGDTHLGGED FDSRLVNFLA
     EEFKRKNKKD LTTNQRSLRR LRTAAERAKR TLSSSAQTSI EIDSLFEGID FYTSITRARF
     EELCADLFRS TLEPVEKVLA DSKLDKSQID EIVLVGGSTR IPKVQKLVSD FFNGKEPNRS
     INPDEAVAYG AAVQAAILTG DQSSTTQDLL LLDVAPLSLG IETAGGIMTK LIPRNSTIPT
     KKSEVFSTYA DNQPGVLIQV FEGERTRTKD NNLLGKFELS GIPPAPRGVP QIEVTFDIDA
     NGILNVSAVE KGTGKSNKIT ITNDKGRLSK EDIDKMVAEA EKFKAEDEQE AQRVQAKNQL
     ESYAFTLKNS VSENNFKEKV GEEDARKLEA AAQDAINWLD ASQAASTEEY KERQKELEGV
     ANPIMSKFYG AAGGAPGAGP VPGAGAGPTG APDNGPTVEE VD
//