ID LIP_BURCE STANDARD; PRT; 364 AA. AC P22088; DT 01-AUG-1991 (REL. 19, CREATED) DT 01-FEB-1996 (REL. 33, LAST SEQUENCE UPDATE) DT 01-NOV-1997 (REL. 35, LAST ANNOTATION UPDATE) DE LIPASE PRECURSOR (EC 3.1.1.3) (TRIACYLGLYCEROL LIPASE). GN LIPA. OS BURKHOLDERIA CEPACIA (PSEUDOMONAS CEPACIA). OC PROKARYOTA; GRACILICUTES; SCOTOBACTERIA; AEROBIC RODS AND COCCI; OC PSEUDOMONADACEAE. RN [1] RP SEQUENCE FROM N.A., AND SEQUENCE OF 45-66. RC STRAIN=DSM 3959; RX MEDLINE; 91100343. RA JORGENSEN S., SKOV K.W., DIDERICHSEN B.; RL J. BACTERIOL. 173:559-567(1991). RN [2] RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS). RX MEDLINE; 97184684. RA KIM K.K., SONG H.K., SHIN D.H., HWANG K.Y., SUH S.W.; RL STRUCTURE 5:173-185(1997). RN [3] RP X-RAY CRYSTALLOGRAPHY. RX MEDLINE; 97184685. RA SCHRAG J.D., LI Y., CYGLER M., LANG D., BURGDORF T., HECHT H.-J., RA SCHMID R., SCHOMBURG D., RYDEL T.J., OLIVER J.D., STRICKLAND L.C., RA DUNAWAY C.M., LARSON S.B., DAY J., MCPHERSON A.; RL STRUCTURE 5:187-202(1997). CC -!- CATALYTIC ACTIVITY: TRIACYLGLYCEROL + H(2)O = DIACYLGLYCEROL + CC A FATTY ACID ANION. CC -!- COFACTOR: CALCIUM IS NECESSARY FOR ENZYMATIC ACTIVITY. CC -!- SIMILARITY: STRONG TO OTHER PSEUDOMONAS LIPASES. CC -!- SIMILARITY: PARTIAL WITH OTHER LIPASES (PANCREATIC, GASTRIC, CC HEPATIC, LINGUAL, LIPOPROTEIN, BACTERIAL, ETC.). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M58494; G557867; -. DR PIR; A39133; A39133. DR PDB; 1OIL; 15-MAY-97. DR PDB; 2LIP; 12-MAR-97. DR PROSITE; PS00120; LIPASE_SER; 1. KW HYDROLASE; LIPID DEGRADATION; SIGNAL; CALCIUM; 3D-STRUCTURE. FT SIGNAL 1 44 FT CHAIN 45 364 LIPASE. FT ACT_SITE 131 131 CHARGE RELAY SYSTEM. FT ACT_SITE 286 286 CHARGE RELAY SYSTEM. FT ACT_SITE 330 330 CHARGE RELAY SYSTEM. SQ SEQUENCE 364 AA; 37494 MW; D030832A CRC32; MARTMRSRVV AGAVACAMSI APFAGTTAVM TLATTHAAMA ATAPAAGYAA TRYPIILVHG LSGTDKYAGV LEYWYGIQED LQQNGATVYV ANLSGFQSDD GPNGRGEQLL AYVKTVLAAT GATKVNLVGH SQGGLSSRYV AAVAPDLVAS VTTIGTPHRG SEFADFVQDV LAYDPTGLSS SVIAAFVNVF GILTSSSHNT NQDALAALQT LTTARAATYN QNYPSAGLGA PGSCQTGAPT ETVGGNTHLL YSWAGTAIQP TLSVFGVTGA TDTSTLPLVD PANVLDLSTL ALFGTGTVMI NRGSGQNDGL VSKCSALYGK VLSTSYKWNH LDEINQLLGV RGAYAEDPVA VIRTHANRLK LAGV //