ID VA16_VACCC Reviewed; 377 AA. AC P20993; DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot. DT 30-AUG-2005, sequence version 2. DT 28-NOV-2006, entry version 29. DE Protein A16. GN ORFNames=A16L; OS Vaccinia virus (strain Copenhagen) (VACV). OC Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae; OC Orthopoxvirus. OX NCBI_TaxID=10249; OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX MEDLINE=91021027; PubMed=2219722; RA Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., RA Paoletti E.; RT "The complete DNA sequence of vaccinia virus."; RL Virology 179:247-266(1990). RN [2] RP COMPLETE GENOME. RA Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., RA Paoletti E.; RT "Appendix to 'The complete DNA sequence of vaccinia virus'."; RL Virology 179:517-563(1990). RN [3] RP MYRISTOYLATION AT GLY-1, SUBCELLULAR LOCATION, AND MUTAGENESIS OF RP GLY-1. RX MEDLINE=97332355; PubMed=9188589; RA Martin K.H., Grosenbach D.W., Franke C.A., Hruby D.E.; RT "Identification and analysis of three myristylated vaccinia virus late RT proteins."; RL J. Virol. 71:5218-5226(1997). CC -!- FUNCTION: Required for virus entry into host cell and for cell- CC cell fusion (syncytium formation) (By similarity). CC -!- SUBUNIT: Part of a stable complex which is at least composed of CC proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation of the CC viral membrane is necessary for the assembly of the complex (By CC similarity). CC -!- SUBCELLULAR LOCATION: Membrane; single-pass type II membrane CC protein (Potential). Component of the intracellular mature virion CC (IMV) outer membrane (By similarity). CC -!- PTM: Most cysteines are linked by disulfide bonds. They are CC created by the viral disulfide bond formation pathway, a poxvirus- CC specific redox pathway that operates on the cytoplasmic side of CC the IMV membranes (By similarity). CC -!- SIMILARITY: Belongs to the poxviruses A16/G9/J5 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M35027; AAA48138.1; -; Genomic_DNA. DR PIR; I42518; I42518. DR InterPro; IPR004251; DUF230_poxv. DR Pfam; PF03003; DUF230; 1. KW Lipoprotein; Membrane; Myristate; Signal-anchor; Transmembrane; KW Virion protein. FT INIT_MET 0 0 FT CHAIN 1 377 Protein A16. FT /FTId=PRO_0000099251. FT TOPO_DOM 1 341 Cytoplasmic (Potential). FT TRANSMEM 342 362 Signal-anchor for type II membrane FT protein (Potential). FT TOPO_DOM 363 377 Lumenal (Potential). FT LIPID 1 1 N-myristoyl glycine (by host). FT MUTAGEN 1 1 G->A: Complete loss of myristoylation. SQ SEQUENCE 377 AA; 43431 MW; BDD7195151C32320 CRC64; GAAVTLNRIK IAPGIADIRD KYMELGFNYP EYNRAVKFAE ESYTYYYETS PGEIKPKFCL IDGMSIDHCS SFIVPEFAKQ YVLIHGEPCS SFKFRPGSLI YYQNEVTPEY IKDLKHATDY IASGQRCHFI KKDYLLGDSD SVAKCCSKTN TKHCPKIFNN NYKTEHCDDF MTGFCRNDPG NPNCLEWLRA KRKPAMSTYS DICSKHMDAR YCSEFIRIIR PDYFTFGDTA LYVFCNDHKG NRNCWCANYP KSNSGDKYLG PRVCWLHECT DESRDRKWLY YNQDVQRTRC KYVGCTINVN SLALKNSQAE LTSNCTRTTS AVGDVHHPGE PVVKDKIKLP TWLGAAITLV VISVIFYFIS IYSRPKIKTN DINVRRR //