ID   PHOS_BOVIN     STANDARD;      PRT;   245 AA.
AC   P19632; P20940; Q28160;
DT   01-FEB-1991 (Rel. 17, Created)
DT   01-MAY-1991 (Rel. 18, Last sequence update)
DT   07-FEB-2006 (Rel. 49, Last annotation update)
DE   Phosducin (PHD) (33 kDa phototransducing protein) (MEKA protein).
GN   Name=PDC;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   PROTEIN SEQUENCE, AND NUCLEOTIDE SEQUENCE OF 228-245.
RC   TISSUE=Retina;
RX   MEDLINE=90368806; PubMed=2203790;
RA   Lee R.H., Fowler A., McGinnis J.F., Lolley R.N., Craft C.M.;
RT   "Amino acid and cDNA sequence of bovine phosducin, a soluble
RT   phosphoprotein from photoreceptor cells.";
RL   J. Biol. Chem. 265:15867-15873(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=89364083; PubMed=2770450; DOI=10.1016/0169-328X(89)90022-3;
RA   Kuo C.-H., Akiyama M., Miki N.;
RT   "Isolation of a novel retina-specific clone (MEKA cDNA) encoding a
RT   photoreceptor soluble protein.";
RL   Brain Res. Mol. Brain Res. 6:1-10(1989).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 8-245.
RC   TISSUE=Pineal gland, and Retina;
RX   MEDLINE=91007277; PubMed=2210381; DOI=10.1016/0378-1119(90)90090-E;
RA   Abe T., Nakabayashi H., Tamada H., Takagi T., Sakuragi S., Yamaki K.,
RA   Shinohara T.;
RT   "Analysis of the human, bovine and rat 33-kDa proteins and cDNA in
RT   retina and pineal gland.";
RL   Gene 91:209-215(1990).
RN   [4]
RP   PHOSPHORYLATION SITE SER-73.
RX   MEDLINE=90368805; PubMed=2394752;
RA   Lee R.H., Brown B.M., Lolley R.N.;
RT   "Protein kinase A phosphorylates retinal phosducin on serine 73 in
RT   situ.";
RL   J. Biol. Chem. 265:15860-15866(1990).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF COMPLEX WITH G-BETA AND
RP   G-GAMMA.
RX   MEDLINE=98416696; PubMed=9739091; DOI=10.1016/S0969-2126(98)00102-6;
RA   Loew A., Ho Y.K., Blundell T., Bax B.;
RT   "Phosducin induces a structural change in transducin beta gamma.";
RL   Structure 6:1007-1019(1998).
CC   -!- FUNCTION: May participate in the regulation of visual
CC       phototransduction or in the integration of photoreceptor
CC       metabolism.
CC   -!- SUBUNIT: Forms a complex with the beta and gamma subunits of the
CC       GTP-binding protein, transducin.
CC   -!- SUBCELLULAR LOCATION: Outer and inner segments of the rod cells.
CC   -!- PTM: Light-induced changes in cyclic nucleotide levels modulate
CC       the phosphorylation of this protein by cAMP kinase (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the phosducin family.
CC   -!- CAUTION: Ref.2 sequence differs from that shown in the C-terminus
CC       due to a frameshift.
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DR   EMBL; M58170; AAA62716.1; -; mRNA.
DR   EMBL; M33529; AAA30349.1; -; mRNA.
DR   PIR; A38379; A38379.
DR   PDB; 1A0R; X-ray; P=1-245.
DR   SMR; P19632; 14-230.
DR   InterPro; IPR001200; Phosducin.
DR   InterPro; IPR006663; Thioredox_dom2.
DR   InterPro; IPR012336; Thioredoxin-like.
DR   InterPro; IPR012335; Thioredoxin_fold.
DR   Pfam; PF02114; Phosducin; 1.
DR   PRINTS; PR00677; PHOSDUCIN.
KW   3D-structure; Direct protein sequencing; Phosphorylation;
KW   Sensory transduction; Vision.
FT   MOD_RES      73     73       Phosphoserine (by PKA).
FT   CONFLICT     44     44       H -> P (in Ref. 3).
FT   CONFLICT    238    239       TN -> SK (in Ref. 3).
FT   HELIX        21     35
FT   TURN         36     36
FT   HELIX        74     80
FT   TURN         81     82
FT   HELIX        87    105
FT   STRAND      114    116
FT   HELIX       120    128
FT   TURN        132    133
FT   STRAND      135    141
FT   TURN        143    144
FT   TURN        146    147
FT   HELIX       148    161
FT   TURN        163    164
FT   STRAND      166    171
FT   HELIX       172    175
FT   TURN        178    179
FT   TURN        183    185
FT   STRAND      188    193
FT   TURN        194    195
FT   STRAND      196    201
FT   TURN        202    203
FT   HELIX       204    207
FT   TURN        210    211
FT   HELIX       214    222
FT   TURN        223    225
SQ   SEQUENCE   245 AA;  28231 MW;  5CA621610401D550 CRC64;
     MEKAKSQSLE EDFEGQASHT GPKGVINDWR KFKLESEDSD SVAHSKKEIL RQMSSPQSRD
     DKDSKERFSR KMSVQEYELI HKDKEDENCL RKYRRQCMQD MHQKLSFGPR YGFVYELESG
     EQFLETIEKE QKITTIVVHI YEDGIKGCDA LNSSLICLAA EYPMVKFCKI KASNTGAGDR
     FSSDVLPTLL VYKGGELLSN FISVTEQLAE EFFTGDVESF LNEYGLLPEK EMHVLEQTNM
     EEDME
//