ID ACON$YEAST STANDARD; PRT; 779 AA. AC P19414; DT 01-NOV-1990 (REL. 16, CREATED) DT 01-NOV-1990 (REL. 16, LAST SEQUENCE UPDATE) DT 01-MAY-1991 (REL. 18, LAST ANNOTATION UPDATE) DE ACONITATE HYDRATASE PRECURSOR (EC 4.2.1.3) (CITRATE HYDRO-LYASE) DE (ACONITASE) (GENE NAME: ACO1). OS SACCHAROMYCES CEREVISIAE (BAKER'S YEAST). OC EUKARYOTA; FUNGI; ASCOMYCOTINA; HEMIASCOMYCETES. RN [1] (STRAIN DBY747, SEQUENCE FROM N.A.) RA GANGLOFF S.P., MARGUET D., LAUQUIN G.J.-M.; RL MOL. CELL. BIOL. 10:3551-3561(1990). CC -!- FUNCTION: REQUIRED FOR GROWTH ON NONFERMENTABLE CARBON SOURCES AND CC FOR BIOSYNTHESIS OF GLUTAMATE. CC -!- CATALYTIC ACTIVITY: CITRATE = CIS-ACONITATE + H(2)O. CC -!- PATHWAY: TRICARBOXYLIC ACID CYCLE. CC -!- ACONITASE HAS AN ACTIVE (4FE-4S) AND AN INACTIVE (3FE-4S) FORMS. CC THE ACTIVE (4FE-4S) CLUSTER IS PART OF THE CATALYTIC SITE THAT CC INTERCONVERTS CITRATE, CIS-ACONITASE, AND ISOCITRATE. CC -!- SUBCELLULAR LOCATION: MITOCHONDRIAL AND EXTRAMITOCHONDRIAL. CC -!- ENZYME REGULATION: SUBJECT TO CATABOLITE REGULATION. CC -!- SIMILARITY: TO OTHER ACONITASES, TO IPM ISOMERASE, AND TO IRE- CC BINDING PROTEIN. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M33131; SCACO1A. DR PIR; A35668; A35668. DR PROSITE; PS00450; ACONITASE. KW LYASE; TRICARBOXYLIC ACID CYCLE; IRON-SULFUR; MITOCHONDRION; KW TRANSIT PEPTIDE; 4FE-4S. FT TRANSIT 1 ? MITOCHONDRION. FT CHAIN ? 779 ACONITASE. FT METAL 382 382 IRON-SULFUR (4FE-4S) (BY SIMILARITY). FT METAL 445 445 IRON-SULFUR (4FE-4S) (BY SIMILARITY). FT METAL 448 448 IRON-SULFUR (4FE-4S) (BY SIMILARITY). SQ SEQUENCE 779 AA; 85749 MW; 3057908 CN; MLSARSAIKR PIVRGLATVS NLTRDSKVNQ NLLEDHSFIN YKQNVETLDI VRKRLNRPFT YAEKILYGHL DDPHGQDIQR GVSYLKLRPD RVACQDATAQ MAILQFMSAG LPQVAKPVTV HCDHLIQAQV GGEKDLKRAI DLNKEVYDFL ASATAKYNMG FWKPGSGIIH QIVLENYAFP GALIIGTDSH TPNAGGLGQL AIGVGGADAV DVMAGRPWEL KAPKILGVKL TGKMNGWTSP KDIILKLAGI TTVKGGTGKI VEYFGDGVDT FSATGMGTIC NMGAEIGATT SVFPFNKSMI EYLEATGRGK IADFAKLYHK DLLSADKDAE YDEVVEIDLN TLEPYINGPF TPDLATPVSK MKEVAVANNW PLDVRVGLIG SCTNSSYEDM SRSASIVKDA AAHGLKSKTI FTVTPGSEQI RATIERDGQL ETFKEFGGIV LANACGPCIG QWDRRDIKKG DKNTIVSSYN RNFTSRNDGN PQTHAFVASP ELVTAFAIAG DLRFNPLTDK LKDKDGNEFM LKPPHGRWFA SKEVMMLVRT LTKLHLQTVA TVEVKVSPTS DRLQLLKPFK PWDGKDAKDM PILIKAVGKT TTDHISMAGP WLKYRGHLEN ISNNYMIGAI NAENKKANCV KNVYTGEYKG VPDTARDYRD QGIKWVVIGD ENFGEGSSRE HAALEPRFLG GFAIITKSFA RIHETNLKKQ GLLPLNFKNP ADYDKINPDD RIDILGLAEL APGKPVTMRV HPKNGKPWDA VLTHTFNDEQ IEWFKYGSAL NKIKADEKK //