ID ACON_YEAST STANDARD; PRT; 778 AA. AC P19414; DT 01-NOV-1990 (REL. 16, CREATED) DT 01-NOV-1995 (REL. 32, LAST SEQUENCE UPDATE) DT 15-DEC-1998 (REL. 37, LAST ANNOTATION UPDATE) DE ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (EC 4.2.1.3) (CITRATE DE HYDRO-LYASE) (ACONITASE). GN ACO1 OR GLU1 OR YLR304C OR L8003.22. OS SACCHAROMYCES CEREVISIAE (BAKER'S YEAST). OC EUKARYOTA; FUNGI; ASCOMYCOTA; HEMIASCOMYCETES; SACCHAROMYCETALES; OC SACCHAROMYCETACEAE; SACCHAROMYCES. RN [1] RP SEQUENCE FROM N.A. RC STRAIN=DBY747; RX MEDLINE; 90287144. RA GANGLOFF S.P., MARGUET D., LAUQUIN G.J.-M.; RT "Molecular cloning of the yeast mitochondrial aconitase gene (ACO1) RT and evidence of a synergistic regulation of expression by glucose RT plus glutamate."; RL MOL. CELL. BIOL. 10:3551-3561(1990). RN [2] RP SEQUENCE FROM N.A. RC STRAIN=S288C / AB972; RA JOHNSTON M., ANDREWS S., BRINKMAN R., COOPER J., DING H., DU Z., RA FAVELLO A., FULTON L., GATTUNG S., GRECO T., KIRSTEN J., RA KUCABA T., HALLSWORTH K., HAWKINS J., HILLIER L., JIER M., RA JOHNSON D., JOHNSTON L., LANGSTON Y., LATREILLE P., LE T., RA MARDIS E., MENEZES S., MILLER N., NHAN M., PAULEY A., PELUSO D., RA RIFKEN L., RILES L., TAICH A., TREVASKIS E., VIGNATI D., RA WILCOX L., WOHLDMAN P., VAUDIN M., WILSON R., WATERSTON R.; RL SUBMITTED (NOV-1994) TO EMBL/GENBANK/DDBJ DATA BANKS. CC -!- FUNCTION: REQUIRED FOR GROWTH ON NONFERMENTABLE CARBON SOURCES AND CC FOR BIOSYNTHESIS OF GLUTAMATE. CC -!- CATALYTIC ACTIVITY: CITRATE = CIS-ACONITATE + H(2)O. CC -!- ENZYME REGULATION: SUBJECT TO CATABOLITE REGULATION. CC -!- PATHWAY: TRICARBOXYLIC ACID CYCLE. CC -!- SUBUNIT: MONOMER. CC -!- SUBCELLULAR LOCATION: MITOCHONDRIAL AND EXTRAMITOCHONDRIAL. CC -!- COFACTOR: ACONITASE HAS AN ACTIVE (4FE-4S) AND AN INACTIVE (3FE- CC 4S) FORMS. THE ACTIVE (4FE-4S) CLUSTER IS PART OF THE CATALYTIC CC SITE THAT INTERCONVERTS CITRATE, CIS-ACONITASE, AND ISOCITRATE (BY CC SIMILARITY). CC -!- SIMILARITY: BELONGS TO THE ACONITASE/IPM ISOMERASE FAMILY. CC -------------------------------------------------------------------------- CC This SWISS-PROT entry is copyright. It is produced through a collaboration CC between the Swiss Institute of Bioinformatics and the EMBL outstation - CC the European Bioinformatics Institute. There are no restrictions on its CC use by non-profit institutions as long as its content is in no way CC modified and this statement is not removed. Usage by and for commercial CC entities requires a license agreement (See http://www.isb-sib.ch/announce/ CC or send an email to license@isb-sib.ch). CC -------------------------------------------------------------------------- CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M33131; G170982; -. DR EMBL; U17243; G596052; -. DR PIR; A35668; A35668. DR SGD; L0000022; ACO1. DR PROSITE; PS00450; ACONITASE_1; 1. DR PROSITE; PS01244; ACONITASE_2; 1. DR PFAM; PF00330; aconitase; 1. DR PFAM; PF00694; Aconitase_C; 1. DR HSSP; P16276; 6ACN. KW LYASE; TRICARBOXYLIC ACID CYCLE; IRON-SULFUR; MITOCHONDRION; KW TRANSIT PEPTIDE; 4FE-4S. FT TRANSIT 1 ? MITOCHONDRION. FT CHAIN ? 778 ACONITASE. FT METAL 382 382 IRON-SULFUR (4FE-4S) (BY SIMILARITY). FT METAL 445 445 IRON-SULFUR (4FE-4S) (BY SIMILARITY). FT METAL 448 448 IRON-SULFUR (4FE-4S) (BY SIMILARITY). FT CONFLICT 527 549 DGLPQRGYDAGENTYQAPPADRS -> RWFASKEVMMLVRT FT LTKLHLQTVA (IN REF. 1). SQ SEQUENCE 778 AA; 85368 MW; EDD2ECA1 CRC32; MLSARSAIKR PIVRGLATVS NLTRDSKVNQ NLLEDHSFIN YKQNVETLDI VRKRLNRPFT YAEKILYGHL DDPHGQDIQR GVSYLKLRPD RVACQDATAQ MAILQFMSAG LPQVAKPVTV HCDHLIQAQV GGEKDLKRAI DLNKEVYDFL ASATAKYNMG FWKPGSGIIH QIVLENYAFP GALIIGTDSH TPNAGGLGQL AIGVGGADAV DVMAGRPWEL KAPKILGVKL TGKMNGWTSP KDIILKLAGI TTVKGGTGKI VEYFGDGVDT FSATGMGTIC NMGAEIGATT SVFPFNKSMI EYLEATGRGK IADFAKLYHK DLLSADKDAE YDEVVEIDLN TLEPYINGPF TPDLATPVSK MKEVAVANNW PLDVRVGLIG SCTNSSYEDM SRSASIVKDA AAHGLKSKTI FTVTPGSEQI RATIERDGQL ETFKEFGGIV LANACGPCIG QWDRRDIKKG DKNTIVSSYN RNFTSRNDGN PQTHAFVASP ELVTAFAIAG DLRFNPLTDK LKDKDGNEFM LKPPHGDGLP QRGYDAGENT YQAPPADRST VEVKVSPTSD RLQLLKPFKP WDGKDAKDMP ILIKAVGKTT TDHISMAGPW LKYRGHLENI SNNYMIGAIN AENKKANCVK NVYTGEYKGV PDTARDYRDQ GIKWVVIGDE NFGEGSSREH AALEPRFLGG FAIITKSFAR IHETNLKKQG LLPLNFKNPA DYDKINPDDR IDILGLAELA PGKPVTMRVH PKNGKPWDAV LTHTFNDEQI EWFKYGSALN KIKADEKK //