ID GGT_ECOLI STANDARD; PRT; 580 AA. AC P18956; DT 01-NOV-1990 (REL. 16, CREATED) DT 01-NOV-1990 (REL. 16, LAST SEQUENCE UPDATE) DT 01-FEB-1991 (REL. 17, LAST ANNOTATION UPDATE) DE GAMMA-GLUTAMYLTRANSPEPTIDASE PRECURSOR (EC 2.3.2.2). GN GGT. OS ESCHERICHIA COLI. OC PROKARYOTA; GRACILICUTES; SCOTOBACTERIA; FACULTATIVELY ANAEROBIC RODS; OC ENTEROBACTERIACEAE. RN [1] RP SEQUENCE FROM N.A., AND SEQUENCE OF 26-41 AND 391-403. RC STRAIN=K12; RM 89359163 RA SUZUKI H., KUMAGAI H., ECHIGO T., TOCHIKURA T.; RL J. BACTERIOL. 171:5169-5172(1989). CC -!- PATHWAY: GGT PLAYS A KEY ROLE IN THE GAMMA-GLUTAMYL CYCLE, A CC PATHWAY FOR THE SYNTHESIS AND DEGRADATION OF GLUTATHIONE. CC -!- CATALYTIC ACTIVITY: (5-L-GLUTAMYL)-PEPTIDE + AN AMINO ACID = CC PEPTIDE + 5-L-GLUTAMYL-AMINO ACID. CC -!- SUBUNIT: THIS ENZYME CONSISTS OF TWO POLYPEPTIDE CHAINS, WHICH CC ARE SYNTHESIZED AS A PRECURSOR FORM OF A SINGLE POLYPEPTIDE. CC -!- SUBCELLULAR LOCATION: PERIPLASMIC. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M28722; M28722. DR PIR; JV0028; EKECEX. DR ECOGENE; EG10374; GGT. DR PROSITE; PS00462; G_GLU_TRANSPEPTIDASE. KW TRANSFERASE; ACYLTRANSFERASE; PERIPLASMIC; ZYMOGEN; SIGNAL; KW GLUTATHIONE BIOSYNTHESIS. FT SIGNAL 1 25 FT CHAIN 26 390 GAMMA-GLUTAMYLTRANSFERASE LARGE CHAIN. FT CHAIN 391 580 GAMMA-GLUTAMYLTRANSFERASE SMALL CHAIN. FT BINDING 465 465 GAMMA-GLUTAMYL (POTENTIAL). SQ SEQUENCE 580 AA; 61768 MW; 1755394 CN; MIKPTFLRRV AIAALLSGSC FSAAAAPPAP PVSYGVEEDV FHPVRAKQGM VASVDATATQ VGVDILKEGG NAVDAAVAVG YALAVTHPQA GNLGGGGFML IRSKNGNTTA IDFREMAPAK ATRDMFLDDQ GNPDSKKSLT SHLASGTPGT VAGFSLALDK YGTMPLNKVV QPAFKLARDG FIVNDALADD LKTYGSEVLP NHENSKAIFW KEGEPLKKGD TLVQANLAKS LEMIAENGPD EFYKGTIAEQ IAQEMQKNGG LITKEDLAAY KAVERTPISG DYRGYQVYSM PPPSSGGIHI VQILNILENF DMKKYGFGSA DAMQIMAEAE KYAYADRSEY LGDPDFVKVP WQALTNKAYA KSIADQIDIN KAKPSSEIRP GKLAPYESNQ TTHYSVVDKD GNAVAVTYTL NTTFGTGIVA GESGILLNNQ MDDFSAKPGV PNVYGLVGGD ANAVGPNKRP LSSMSPTIVV KDGKTWLVTG SPGGSRIITT VLQMVVNSID YGLNVAEATN APRFHHQWLP DELRVEKGFS PDTLKLLEAK GQKVALKEAM GSTQSIMVGP DGELYGASDP RSVDDLTAGY //