ID HGF_RAT Reviewed; 728 AA. AC P17945; DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1990, sequence version 1. DT 26-JUN-2013, entry version 118. DE RecName: Full=Hepatocyte growth factor; DE AltName: Full=Hepatopoietin-A; DE AltName: Full=Scatter factor; DE Short=SF; DE Contains: DE RecName: Full=Hepatocyte growth factor alpha chain; DE Contains: DE RecName: Full=Hepatocyte growth factor beta chain; DE Flags: Precursor; GN Name=Hgf; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Rattus. OX NCBI_TaxID=10116; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE. RC STRAIN=Wistar; TISSUE=Liver; RX PubMed=2139229; DOI=10.1073/pnas.87.8.3200; RA Toshiro K., Hagiya M., Nishizawa T., Seki T., Shimonishi M., RA Shimizu S., Nakamura T.; RT "Deduced primary structure of rat hepatocyte growth factor and RT expression of the mRNA in rat tissues."; RL Proc. Natl. Acad. Sci. U.S.A. 87:3200-3204(1990). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=Wistar; TISSUE=Liver; RX PubMed=2146117; DOI=10.1111/j.1432-1033.1990.tb19349.x; RA Okajima A., Miyazawa K., Kitamura N.; RT "Primary structure of rat hepatocyte growth factor and induction of RT its mRNA during liver regeneration following hepatic injury."; RL Eur. J. Biochem. 193:375-381(1990). CC -!- FUNCTION: Potent mitogen for mature parenchymal hepatocyte cells, CC seems to be a hepatotrophic factor, and acts as a growth factor CC for a broad spectrum of tissues and cell types. Activating ligand CC for the receptor tyrosine kinase MET by binding to it and CC promoting its dimerization (By similarity). CC -!- SUBUNIT: Dimer of an alpha chain and a beta chain linked by a CC disulfide bond. CC -!- SIMILARITY: Belongs to the peptidase S1 family. Plasminogen CC subfamily. CC -!- SIMILARITY: Contains 4 kringle domains. CC -!- SIMILARITY: Contains 1 PAN domain. CC -!- SIMILARITY: Contains 1 peptidase S1 domain. CC -!- CAUTION: Has lost two of the three essential catalytic residues CC and so probably has no enzymatic activity. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D90102; BAA14133.1; -; mRNA. DR EMBL; X54400; CAA38266.1; -; mRNA. DR IPI; IPI00192939; -. DR PIR; A35644; A35644. DR RefSeq; NP_058713.1; NM_017017.2. DR UniGene; Rn.10468; -. DR ProteinModelPortal; P17945; -. DR SMR; P17945; 39-210, 496-725. DR MINT; MINT-219643; -. DR STRING; 10116.ENSRNOP00000009764; -. DR MEROPS; S01.978; -. DR PaxDb; P17945; -. DR PRIDE; P17945; -. DR Ensembl; ENSRNOT00000009763; ENSRNOP00000009764; ENSRNOG00000007027. DR GeneID; 24446; -. DR KEGG; rno:24446; -. DR CTD; 3082; -. DR RGD; 2794; Hgf. DR eggNOG; COG5640; -. DR GeneTree; ENSGT00700000104191; -. DR HOGENOM; HOG000112892; -. DR HOVERGEN; HBG004381; -. DR InParanoid; P17945; -. DR KO; K05460; -. DR OMA; GSESPWC; -. DR OrthoDB; EOG4R7V94; -. DR NextBio; 603343; -. DR PMAP-CutDB; P17945; -. DR Genevestigator; P17945; -. DR GermOnline; ENSRNOG00000007027; Rattus norvegicus. DR GO; GO:0005615; C:extracellular space; IDA:RGD. DR GO; GO:0042056; F:chemoattractant activity; IEA:Compara. DR GO; GO:0008083; F:growth factor activity; IMP:RGD. DR GO; GO:0005171; F:hepatocyte growth factor receptor binding; TAS:RGD. DR GO; GO:0046982; F:protein heterodimerization activity; IDA:RGD. DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro. DR GO; GO:0000187; P:activation of MAPK activity; IEA:Compara. DR GO; GO:0060326; P:cell chemotaxis; IEA:Compara. DR GO; GO:0000902; P:cell morphogenesis; IEA:Compara. DR GO; GO:0008283; P:cell proliferation; TAS:RGD. DR GO; GO:0006928; P:cellular component movement; TAS:RGD. DR GO; GO:0035729; P:cellular response to hepatocyte growth factor stimulus; IEA:Compara. DR GO; GO:0048012; P:hepatocyte growth factor receptor signaling pathway; IEA:Compara. DR GO; GO:0030212; P:hyaluronan metabolic process; IEP:RGD. DR GO; GO:0001889; P:liver development; IEA:Compara. DR GO; GO:0051450; P:myoblast proliferation; IEA:Compara. DR GO; GO:0043066; P:negative regulation of apoptotic process; IDA:RGD. DR GO; GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara. DR GO; GO:1901299; P:negative regulation of hydrogen peroxide-mediated programmed cell death; IEA:Compara. DR GO; GO:0090201; P:negative regulation of release of cytochrome c from mitochondria; IEA:Compara. DR GO; GO:0031100; P:organ regeneration; IEP:RGD. DR GO; GO:0030335; P:positive regulation of cell migration; IEA:Compara. DR GO; GO:0008284; P:positive regulation of cell proliferation; TAS:RGD. DR GO; GO:2000573; P:positive regulation of DNA biosynthetic process; IEA:Compara. DR GO; GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IEA:Compara. DR GO; GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; IEA:Compara. DR GO; GO:0006508; P:proteolysis; IEA:InterPro. DR GO; GO:0060665; P:regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling; IEA:Compara. DR Gene3D; 2.40.20.10; -; 4. DR InterPro; IPR027284; Hepatocyte_GF. DR InterPro; IPR024174; HGF_MST1. DR InterPro; IPR000001; Kringle. DR InterPro; IPR013806; Kringle-like. DR InterPro; IPR018056; Kringle_CS. DR InterPro; IPR003014; PAN-1_domain. DR InterPro; IPR003609; Pan_app. DR InterPro; IPR001254; Peptidase_S1. DR InterPro; IPR001314; Peptidase_S1A. DR InterPro; IPR009003; Trypsin-like_Pept_dom. DR Pfam; PF00051; Kringle; 4. DR Pfam; PF00024; PAN_1; 1. DR Pfam; PF00089; Trypsin; 1. DR PIRSF; PIRSF500183; Hepatocyte_GF; 1. DR PIRSF; PIRSF001152; HGF_MST1; 1. DR PRINTS; PR00722; CHYMOTRYPSIN. DR SMART; SM00130; KR; 4. DR SMART; SM00473; PAN_AP; 1. DR SMART; SM00020; Tryp_SPc; 1. DR SUPFAM; SSF57440; Kringle-like; 4. DR SUPFAM; SSF50494; Pept_Ser_Cys; 1. DR PROSITE; PS00021; KRINGLE_1; 4. DR PROSITE; PS50070; KRINGLE_2; 4. DR PROSITE; PS50948; PAN; 1. DR PROSITE; PS50240; TRYPSIN_DOM; 1. PE 1: Evidence at protein level; KW Complete proteome; Direct protein sequencing; Disulfide bond; KW Glycoprotein; Growth factor; Kringle; Pyrrolidone carboxylic acid; KW Reference proteome; Repeat; Serine protease homolog; Signal. FT SIGNAL 1 32 By similarity. FT CHAIN 33 495 Hepatocyte growth factor alpha chain. FT /FTId=PRO_0000028095. FT CHAIN 496 728 Hepatocyte growth factor beta chain. FT /FTId=PRO_0000028096. FT DOMAIN 38 124 PAN. FT DOMAIN 129 207 Kringle 1. FT DOMAIN 212 289 Kringle 2. FT DOMAIN 306 384 Kringle 3. FT DOMAIN 392 470 Kringle 4. FT DOMAIN 496 724 Peptidase S1. FT MOD_RES 33 33 Pyrrolidone carboxylic acid (By FT similarity). FT CARBOHYD 295 295 N-linked (GlcNAc...) (Potential). FT CARBOHYD 403 403 N-linked (GlcNAc...) (Potential). FT CARBOHYD 569 569 N-linked (GlcNAc...) (Potential). FT CARBOHYD 656 656 N-linked (GlcNAc...) (Potential). FT DISULFID 71 97 By similarity. FT DISULFID 75 85 By similarity. FT DISULFID 129 207 By similarity. FT DISULFID 150 190 By similarity. FT DISULFID 178 202 By similarity. FT DISULFID 212 289 By similarity. FT DISULFID 233 272 By similarity. FT DISULFID 261 284 By similarity. FT DISULFID 306 384 By similarity. FT DISULFID 327 366 By similarity. FT DISULFID 355 378 By similarity. FT DISULFID 392 470 By similarity. FT DISULFID 413 453 By similarity. FT DISULFID 441 465 By similarity. FT DISULFID 488 607 Interchain (between alpha and beta FT chains) (By similarity). FT DISULFID 520 536 By similarity. FT DISULFID 615 682 By similarity. FT DISULFID 645 661 By similarity. FT DISULFID 672 700 By similarity. SQ SEQUENCE 728 AA; 82906 MW; 3E0BF1F96ADCEDFF CRC64; MMWGTKLLPV LLLQHVLLHL LLLPVTIPYA EGQKKRRNTL HEFKKSAKTT LTKEDPLVKI KTKKVNSADE CANRCIRNKG FPFTCKAFVF DKSRKRCYWY PFNSMSSGVK KGFGHEFDLY ENKDYIRNCI IGKGGSYKGT VSITKSGIKC QPWNSMIPHE HSFLPSSYRG KDLQENYCRN PRGEEGGPWC FTSNPEVRYE VCDIPQCSEV ECMTCNGESY RGPMDHTESG KTCQRWDQQT PHRHKFLPER YPDKGFDDNY CRNPDGKPRP WCYTLDPDTP WEYCAIKMCA HSAVNETDVP METTECIKGQ GEGYRGTTNT IWNGIPCQRW DSQYPHKHDI TPENFKCKDL RENYCRNPDG AESPWCFTTD PNIRVGYCSQ IPKCDVSSGQ DCYRGNGKNY MGNLSKTRSG LTCSMWDKNM EDLHRHIFWE PDASKLTKNY CRNPDDDAHG PWCYTGNPLV PWDYCPISRC EGDTTPTIVN LDHPVISCAK TKQLRVVNGI PTQTTVGWMV SLKYRNKHIC GGSLIKESWV LTARQCFPAR NKDLKDYEAW LGIHDVHERG EEKRKQILNI SQLVYGPEGS DLVLLKLARP AILDNFVSTI DLPSYGCTIP EKTTCSIYGW GYTGLINADG LLRVAHLYIM GNEKCSQHHQ GKVTLNESEL CAGAEKIGSG PCEGDYGGPL ICEQHKMRMV LGVIVPGRGC AIPNRPGIFV RVAYYAKWIH KVILTYKL //