ID REV_HV2D1 Reviewed; 103 AA. AC P17754; DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1990, sequence version 1. DT 26-FEB-2020, entry version 77. DE RecName: Full=Protein Rev; DE AltName: Full=Regulator of expression of viral proteins; GN Name=rev; OS Human immunodeficiency virus type 2 subtype A (isolate D194) (HIV-2). OC Viruses; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus. OX NCBI_TaxID=11713; OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=2467304; DOI=10.1073/pnas.86.7.2383; RA Kuehnel H., von Briesen H., Dietrich U., Adamski M., Mix D., Biesert L., RA Kreutz R., Immelmann A., Henco K., Meichsner C., Andreesen R., RA Gelderblom H., Ruebsamen-Waigmann H.; RT "Molecular cloning of two west African human immunodeficiency virus type 2 RT isolates that replicate well in macrophages: a Gambian isolate, from a RT patient with neurologic acquired immunodeficiency syndrome, and a highly RT divergent Ghanian isolate."; RL Proc. Natl. Acad. Sci. U.S.A. 86:2383-2387(1989). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=2235509; DOI=10.1093/nar/18.20.6142; RA Kuehnel H., Kreutz R., Ruebsamen-Waigmann H.; RT "Nucleotide sequence of HIV-2D194, an isolate from a Gambian case of RT 'neuro-AIDS', which showed excellent growth in macrophages."; RL Nucleic Acids Res. 18:6142-6142(1990). CC -!- FUNCTION: Escorts unspliced or incompletely spliced viral pre-mRNAs CC (late transcripts) out of the nucleus of infected cells. These pre- CC mRNAs carry a recognition sequence called Rev responsive element (RRE) CC located in the env gene, that is not present in fully spliced viral CC mRNAs (early transcripts). This function is essential since most viral CC proteins are translated from unspliced or partially spliced pre-mRNAs CC which cannot exit the nucleus by the pathway used by fully processed CC cellular mRNAs (By similarity). {ECO:0000250}. CC -!- SUBUNIT: Homomultimer; when bound to the RRE. Multimeric assembly is CC essential for activity (By similarity). {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Host nucleus, host nucleolus. Host cytoplasm. CC Note=The presence of both nuclear import and nuclear export signals CC leads to continuous shuttling between the nucleus and cytoplasm. CC {ECO:0000250}. CC -!- DOMAIN: The RNA-binding motif binds to the RRE, a stem-and-loop CC structure present in incompletely spliced viral pre-mRNAs. This region CC also contains the NLS which mediates nuclear localization. These CC overlapping functions prevent Rev bound to RRE from undesirable return CC to the nucleus. When Rev binds the RRE, the NLS becomes masked while CC the NES remains accessible (By similarity). {ECO:0000250}. CC -!- MISCELLANEOUS: This isolate is from a Gambian case of 'neuro-AIDS'. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; J04542; AAA76846.1; -; Genomic_DNA. DR EMBL; X52223; CAA36470.1; -; Genomic_DNA. DR PIR; S12158; S12158. DR SMR; P17754; -. DR Proteomes; UP000007422; Genome. DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell. DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW. DR InterPro; IPR000625; REV_protein. DR Pfam; PF00424; REV; 1. PE 3: Inferred from homology; KW AIDS; Host cytoplasm; Host nucleus; mRNA transport; RNA-binding; Transport. FT CHAIN 1..103 FT /note="Protein Rev" FT /id="PRO_0000085281" FT REGION 16..24 FT /note="Homomultimerization" FT /evidence="ECO:0000250" FT MOTIF 35..49 FT /note="Nuclear localization signal and RNA-binding (RRE)" FT /evidence="ECO:0000250" FT MOTIF 71..82 FT /note="Nuclear export signal and binding to XPO1" FT /evidence="ECO:0000250" SQ SEQUENCE 103 AA; 11963 MW; 607BEF9A5B36E7A0 CRC64; MRDRADEEGL QEKLRLIRLL HQINPYPHGQ GTASQRRNRR RRRRRQWFRL VALATKLHTI PDPPTDSPLD RAIQQLQGLT IQELPDPPTD LPESNSNQGL AET //