ID REV_HV2D1 Reviewed; 103 AA. AC P17754; DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1990, sequence version 1. DT 03-APR-2013, entry version 65. DE RecName: Full=Protein Rev; DE AltName: Full=Regulator of expression of viral proteins; GN Name=rev; OS Human immunodeficiency virus type 2 subtype A (isolate D194) (HIV-2). OC Viruses; Retro-transcribing viruses; Retroviridae; Orthoretrovirinae; OC Lentivirus; Primate lentivirus group. OX NCBI_TaxID=11713; OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=2467304; DOI=10.1073/pnas.86.7.2383; RA Kuehnel H., von Briesen H., Dietrich U., Adamski M., Mix D., RA Biesert L., Kreutz R., Immelmann A., Henco K., Meichsner C., RA Andreesen R., Gelderblom H., Ruebsamen-Waigmann H.; RT "Molecular cloning of two west African human immunodeficiency virus RT type 2 isolates that replicate well in macrophages: a Gambian isolate, RT from a patient with neurologic acquired immunodeficiency syndrome, and RT a highly divergent Ghanian isolate."; RL Proc. Natl. Acad. Sci. U.S.A. 86:2383-2387(1989). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=2235509; DOI=10.1093/nar/18.20.6142; RA Kuehnel H., Kreutz R., Ruebsamen-Waigmann H.; RT "Nucleotide sequence of HIV-2D194, an isolate from a Gambian case of RT 'neuro-AIDS', which showed excellent growth in macrophages."; RL Nucleic Acids Res. 18:6142-6142(1990). CC -!- FUNCTION: Escorts unspliced or incompletely spliced viral pre- CC mRNAs (late transcripts) out of the nucleus of infected cells. CC These pre-mRNAs carry a recognition sequence called Rev responsive CC element (RRE) located in the env gene, that is not present in CC fully spliced viral mRNAs (early transcripts). This function is CC essential since most viral proteins are translated from unspliced CC or partially spliced pre-mRNAs which cannot exit the nucleus by CC the pathway used by fully processed cellular mRNAs (By CC similarity). CC -!- SUBUNIT: Homomultimer; when bound to the RRE. Multimeric assembly CC is essential for activity (By similarity). CC -!- SUBCELLULAR LOCATION: Host nucleus, host nucleolus. Host CC cytoplasm. Note=The presence of both nuclear import and nuclear CC export signals leads to continuous shuttling between the nucleus CC and cytoplasm (By similarity). CC -!- DOMAIN: The RNA-binding motif binds to the RRE, a stem-and-loop CC structure present in incompletely spliced viral pre-mRNAs. This CC region also contains the NLS which mediates nuclear localization. CC These overlapping functions prevent Rev bound to RRE from CC undesirable return to the nucleus. When Rev binds the RRE, the NLS CC becomes masked while the NES remains accessible (By similarity). CC -!- MISCELLANEOUS: This isolate is from a Gambian case of 'neuro- CC AIDS'. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; J04542; AAA76846.1; -; Genomic_DNA. DR EMBL; X52223; CAA36470.1; -; Genomic_DNA. DR PIR; S12158; S12158. DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro. DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW. DR InterPro; IPR000625; REV_protein. DR Pfam; PF00424; REV; 1. PE 3: Inferred from homology; KW AIDS; Complete proteome; Host cytoplasm; Host nucleus; mRNA transport; KW RNA-binding; Transport. FT CHAIN 1 103 Protein Rev. FT /FTId=PRO_0000085281. FT REGION 16 24 Homomultimerization (By similarity). FT MOTIF 35 49 Nuclear localization signal and RNA- FT binding (RRE) (By similarity). FT MOTIF 71 82 Nuclear export signal and binding to XPO1 FT (By similarity). SQ SEQUENCE 103 AA; 11963 MW; 607BEF9A5B36E7A0 CRC64; MRDRADEEGL QEKLRLIRLL HQINPYPHGQ GTASQRRNRR RRRRRQWFRL VALATKLHTI PDPPTDSPLD RAIQQLQGLT IQELPDPPTD LPESNSNQGL AET //