ID A16_VACCW Reviewed; 377 AA. AC P16710; Q80HV5; DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 4. DT 03-APR-2013, entry version 62. DE RecName: Full=Virion membrane protein A16; GN OrderedLocusNames=VACWR136; ORFNames=A16L; OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain OS WR)). OC Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae; OC Orthopoxvirus; Vaccinia virus. OX NCBI_TaxID=10254; OH NCBI_TaxID=9913; Bos taurus (Bovine). RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., RA Osborne J., Wohlhueter R.; RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold RT redundancy and an error rate of 0.16/10kb."; RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-275. RX PubMed=2370683; RA Pacha R.F., Meis R.J., Condit R.C.; RT "Structure and expression of the vaccinia virus gene which prevents RT virus-induced breakdown of RNA."; RL J. Virol. 64:3853-3863(1990). RN [3] RP MYRISTOYLATION AT GLY-2, SUBCELLULAR LOCATION, AND MUTAGENESIS OF RP GLY-2. RX PubMed=9188589; RA Martin K.H., Grosenbach D.W., Franke C.A., Hruby D.E.; RT "Identification and analysis of three myristylated vaccinia virus late RT proteins."; RL J. Virol. 71:5218-5226(1997). RN [4] RP IDENTIFICATION IN A COMPLEX WITH A21; A28; G3; G9; H2; J5 AND L5. RX PubMed=16339313; DOI=10.1073/pnas.0509239102; RA Senkevich T.G., Ojeda S., Townsley A., Nelson G.E., Moss B.; RT "Poxvirus multiprotein entry-fusion complex."; RL Proc. Natl. Acad. Sci. U.S.A. 102:18572-18577(2005). RN [5] RP FUNCTION, DISULFIDE BONDS, INDUCTION, AND TOPOLOGY. RX PubMed=16352530; DOI=10.1128/JVI.80.1.51-61.2006; RA Ojeda S., Senkevich T.G., Moss B.; RT "Entry of vaccinia virus and cell-cell fusion require a highly RT conserved cysteine-rich membrane protein encoded by the A16L gene."; RL J. Virol. 80:51-61(2006). RN [6] RP INTERACTION WITH G9. RX PubMed=18353946; DOI=10.1128/JVI.00162-08; RA Wagenaar T.R., Ojeda S., Moss B.; RT "Vaccinia virus A56/K2 fusion regulatory protein interacts with the RT A16 and G9 subunits of the entry fusion complex."; RL J. Virol. 82:5153-5160(2008). CC -!- FUNCTION: Envelope protein part of the entry-fusion complex CC responsible for the virus membrane fusion with host cell membrane CC during virus entry. Also plays a role in cell-cell fusion CC (syncytium formation). CC -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at CC least composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. CC Formation of the viral membrane is necessary for the assembly of CC the complex. Interacts with G9. CC -!- SUBCELLULAR LOCATION: Virion membrane; Single-pass type II CC membrane protein (Potential). Note=Component of the mature virion CC (MV) membrane (Probable). The mature virion is located in the CC cytoplasm of infected cells and is probably released by cell CC lysis. CC -!- INDUCTION: Expressed in the late phase of the viral replicative CC cycle. CC -!- PTM: Most cysteines are linked by disulfide bonds. They are CC created by the viral disulfide bond formation pathway, a poxvirus- CC specific redox pathway that operates on the cytoplasmic side of CC the MV membranes. CC -!- SIMILARITY: Belongs to the poxviridae A16/G9/J5 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY243312; AAO89415.1; -; Genomic_DNA. DR EMBL; M32064; AAA48348.2; -; Genomic_DNA. DR PIR; A36415; A36415. DR RefSeq; YP_233018.1; NC_006998.1. DR MINT; MINT-6740541; -. DR GeneID; 3707666; -. DR ProtClustDB; CLSP2509816; -. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW. DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell. DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW. DR InterPro; IPR004251; Vaccinia_virus_A16. DR Pfam; PF03003; DUF230; 1. PE 1: Evidence at protein level; KW Complete proteome; Disulfide bond; KW Fusion of virus membrane with host membrane; Late protein; KW Lipoprotein; Membrane; Myristate; Reference proteome; Signal-anchor; KW Transmembrane; Transmembrane helix; Viral envelope protein; KW Viral penetration into host cytoplasm; Virion; KW Virus entry into host cell. FT INIT_MET 1 1 Removed; by host. FT CHAIN 2 377 Virion membrane protein A16. FT /FTId=PRO_0000099253. FT TOPO_DOM 2 342 Virion surface (Potential). FT TRANSMEM 343 363 Helical; Signal-anchor for type II FT membrane protein; (Potential). FT TOPO_DOM 364 377 Intravirion (Potential). FT LIPID 2 2 N-myristoyl glycine; by host. FT MUTAGEN 2 2 G->A: Complete loss of myristoylation. SQ SEQUENCE 377 AA; 43425 MW; EE79C44446B542FA CRC64; MGAAVTLNRI KIAPGIADIR DKYMELGFNY PEYNRAVKFA EESYTYYYET SPGEIKPKFC LIDGMSIDHC SSFIVPEFAK QYVLIHGEPC SSFKFRPGSL IYYQNEVTPE YIKDLKHATD YIASGQRCHF IKKDYLLGDS DSVAKCCSKT NTKHCPKIFN NNYKTEHCDD FMTGFCRNDP GNPNCLEWLR AKRKPAMSTY SDICSKHMDA RYCSEFIRII RPDYFTFGDT ALYVFCNDHK GNRNCWCANY PKSNSGDKYL GPRVCWLHEC TDESRDRKWL YYNQDVQRTR CKYVGCTINV NSLALKNSQA ELTSNCTRTT SAVGDVHPGE PVVKDKIKLP TWLGAAITLV VISVIFYFIS IYSRPKIKTN DINVRRR //