ID VA16_VACCW Reviewed; 377 AA. AC P16710; Q80HV5; DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 4. DT 19-OCT-2011, entry version 57. DE RecName: Full=Protein A16; GN OrderedLocusNames=VACWR136; ORFNames=A16L; OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain OS WR)). OC Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae; OC Orthopoxvirus; Vaccinia virus. OX NCBI_TaxID=10254; OH NCBI_TaxID=9913; Bos taurus (Bovine). RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., RA Osborne J., Wohlhueter R.; RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold RT redundancy and an error rate of 0.16/10kb."; RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-275. RX MEDLINE=90317884; PubMed=2370683; RA Pacha R.F., Meis R.J., Condit R.C.; RT "Structure and expression of the vaccinia virus gene which prevents RT virus-induced breakdown of RNA."; RL J. Virol. 64:3853-3863(1990). RN [3] RP MYRISTOYLATION AT GLY-2, SUBCELLULAR LOCATION, AND MUTAGENESIS OF RP GLY-2. RX MEDLINE=97332355; PubMed=9188589; RA Martin K.H., Grosenbach D.W., Franke C.A., Hruby D.E.; RT "Identification and analysis of three myristylated vaccinia virus late RT proteins."; RL J. Virol. 71:5218-5226(1997). RN [4] RP IDENTIFICATION IN A COMPLEX WITH A21; A28; G3; G9; H2; J5 AND L5. RX PubMed=16339313; DOI=10.1073/pnas.0509239102; RA Senkevich T.G., Ojeda S., Townsley A., Nelson G.E., Moss B.; RT "Poxvirus multiprotein entry-fusion complex."; RL Proc. Natl. Acad. Sci. U.S.A. 102:18572-18577(2005). RN [5] RP FUNCTION, AND DISULFIDE BONDS. RX PubMed=16352530; DOI=10.1128/JVI.80.1.51-61.2006; RA Ojeda S., Senkevich T.G., Moss B.; RT "Entry of vaccinia virus and cell-cell fusion require a highly RT conserved cysteine-rich membrane protein encoded by the A16L gene."; RL J. Virol. 80:51-61(2006). CC -!- FUNCTION: Required for virus entry into host cell and for cell- CC cell fusion (syncytium formation). CC -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at CC least composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. CC Formation of the viral membrane is necessary for the assembly of CC the complex. CC -!- SUBCELLULAR LOCATION: Virion membrane; Single-pass type II CC membrane protein (Potential). Note=Component of the intracellular CC mature virion (IMV) outer membrane (By similarity). CC -!- PTM: Most cysteines are linked by disulfide bonds. They are CC created by the viral disulfide bond formation pathway, a poxvirus- CC specific redox pathway that operates on the cytoplasmic side of CC the IMV membranes. CC -!- SIMILARITY: Belongs to the poxviruses A16/G9/J5 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY243312; AAO89415.1; -; Genomic_DNA. DR EMBL; M32064; AAA48348.2; -; Genomic_DNA. DR PIR; A36415; A36415. DR RefSeq; YP_233018.1; NC_006998.1. DR MINT; MINT-6740541; -. DR GeneID; 3707666; -. DR ProtClustDB; CLSP2509816; -. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell. DR InterPro; IPR004251; Vaccinia_virus_A16. DR Pfam; PF03003; DUF230; 1. PE 1: Evidence at protein level; KW Complete proteome; Disulfide bond; KW Fusion of virus membrane with host membrane; KW Initiation of viral infection; Lipoprotein; Membrane; Myristate; KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; KW Viral penetration into host cytoplasm; Virion. FT INIT_MET 1 1 Removed; by host. FT CHAIN 2 377 Protein A16. FT /FTId=PRO_0000099253. FT TOPO_DOM 2 342 Cytoplasmic (Potential). FT TRANSMEM 343 363 Helical; Signal-anchor for type II FT membrane protein; (Potential). FT TOPO_DOM 364 377 Lumenal (Potential). FT LIPID 2 2 N-myristoyl glycine; by host. FT MUTAGEN 2 2 G->A: Complete loss of myristoylation. SQ SEQUENCE 377 AA; 43425 MW; EE79C44446B542FA CRC64; MGAAVTLNRI KIAPGIADIR DKYMELGFNY PEYNRAVKFA EESYTYYYET SPGEIKPKFC LIDGMSIDHC SSFIVPEFAK QYVLIHGEPC SSFKFRPGSL IYYQNEVTPE YIKDLKHATD YIASGQRCHF IKKDYLLGDS DSVAKCCSKT NTKHCPKIFN NNYKTEHCDD FMTGFCRNDP GNPNCLEWLR AKRKPAMSTY SDICSKHMDA RYCSEFIRII RPDYFTFGDT ALYVFCNDHK GNRNCWCANY PKSNSGDKYL GPRVCWLHEC TDESRDRKWL YYNQDVQRTR CKYVGCTINV NSLALKNSQA ELTSNCTRTT SAVGDVHPGE PVVKDKIKLP TWLGAAITLV VISVIFYFIS IYSRPKIKTN DINVRRR //