ID KITH_SHEVK Reviewed; 177 AA. AC P16600; DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1990, sequence version 1. DT 29-MAY-2024, entry version 71. DE RecName: Full=Thymidine kinase; DE EC=2.7.1.21; GN Name=TK; OS Sheeppox virus (strain KS-1) (SPPV) (Capripoxvirus (strain KS-1)). OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; OC Chitovirales; Poxviridae; Chordopoxvirinae; Capripoxvirus; Sheeppox virus. OX NCBI_TaxID=10269; OH NCBI_TaxID=9940; Ovis aries (Sheep). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=2732700; DOI=10.1099/0022-1317-70-3-525; RA Gershon P.D., Black D.N.; RT "The nucleotide sequence around the capripoxvirus thymidine kinase gene RT reveals a gene shared specifically with leporipoxvirus."; RL J. Gen. Virol. 70:525-533(1989). CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + thymidine = ADP + dTMP + H(+); Xref=Rhea:RHEA:19129, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; EC=2.7.1.21; CC -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D00423; BAA00324.1; -; Genomic_DNA. DR PIR; B31813; KIVZCP. DR SMR; P16600; -. DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-EC. DR GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW. DR GO; GO:0046104; P:thymidine metabolic process; IEA:TreeGrafter. DR Gene3D; 3.30.60.20; -; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR001267; Thymidine_kinase. DR InterPro; IPR020633; Thymidine_kinase_CS. DR PANTHER; PTHR11441; THYMIDINE KINASE; 1. DR PANTHER; PTHR11441:SF0; THYMIDINE KINASE, CYTOSOLIC; 1. DR Pfam; PF00265; TK; 1. DR PIRSF; PIRSF035805; TK_cell; 1. DR SUPFAM; SSF57716; Glucocorticoid receptor-like (DNA-binding domain); 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR PROSITE; PS00603; TK_CELLULAR_TYPE; 1. PE 3: Inferred from homology; KW ATP-binding; DNA synthesis; Kinase; Metal-binding; Nucleotide-binding; KW Transferase; Zinc. FT CHAIN 1..177 FT /note="Thymidine kinase" FT /id="PRO_0000174929" FT ACT_SITE 83 FT /note="Proton acceptor" FT /evidence="ECO:0000255" FT BINDING 11..18 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000250" FT BINDING 113 FT /ligand="substrate" FT /evidence="ECO:0000250" FT BINDING 138 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000250" FT BINDING 141 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000250" FT BINDING 157..161 FT /ligand="substrate" FT /evidence="ECO:0000250" FT BINDING 170 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000250" FT BINDING 173 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000250" SQ SEQUENCE 177 AA; 20447 MW; 093E53E555F8E2C8 CRC64; MDYGYIHLII GPMFSGKSTE LIRIVKRYQI AQYKCCVVKY LKDIRYGNSV YTHDNNHVSA ISTTLLYDVV DKIMNFDIIG IDEGQFFKDI VSFSENMANM GKIIIIAALD STFQRKEFND ILKLIPLSEK VTKLNAVCME CYKDAAFSKR ITKEKEIELI GGKEKYKSVC RKCYFLE //