ID KITH_SHEVK Reviewed; 177 AA. AC P16600; DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1990, sequence version 1. DT 11-DEC-2019, entry version 64. DE RecName: Full=Thymidine kinase; DE EC=2.7.1.21; GN Name=TK; OS Sheeppox virus (strain KS-1) (SPPV) (Capripoxvirus (strain KS-1)). OC Viruses; Poxviridae; Chordopoxvirinae; Capripoxvirus. OX NCBI_TaxID=10269; OH NCBI_TaxID=9940; Ovis aries (Sheep). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=2732700; DOI=10.1099/0022-1317-70-3-525; RA Gershon P.D., Black D.N.; RT "The nucleotide sequence around the capripoxvirus thymidine kinase gene RT reveals a gene shared specifically with leporipoxvirus."; RL J. Gen. Virol. 70:525-533(1989). CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + thymidine = ADP + dTMP + H(+); Xref=Rhea:RHEA:19129, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; EC=2.7.1.21; CC -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D00423; BAA00324.1; -; Genomic_DNA. DR PIR; B31813; KIVZCP. DR SMR; P16600; -. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-EC. DR GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR001267; Thymidine_kinase. DR InterPro; IPR020633; Thymidine_kinase_CS. DR PANTHER; PTHR11441; PTHR11441; 1. DR Pfam; PF00265; TK; 1. DR PIRSF; PIRSF035805; TK_cell; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS00603; TK_CELLULAR_TYPE; 1. PE 3: Inferred from homology; KW ATP-binding; DNA synthesis; Kinase; Metal-binding; Nucleotide-binding; KW Transferase; Zinc. FT CHAIN 1..177 FT /note="Thymidine kinase" FT /id="PRO_0000174929" FT NP_BIND 11..18 FT /note="ATP" FT /evidence="ECO:0000250" FT REGION 157..161 FT /note="Substrate binding" FT /evidence="ECO:0000250" FT ACT_SITE 83 FT /note="Proton acceptor" FT /evidence="ECO:0000255" FT METAL 138 FT /note="Zinc" FT /evidence="ECO:0000250" FT METAL 141 FT /note="Zinc" FT /evidence="ECO:0000250" FT METAL 170 FT /note="Zinc" FT /evidence="ECO:0000250" FT METAL 173 FT /note="Zinc" FT /evidence="ECO:0000250" FT BINDING 113 FT /note="Substrate; via amide nitrogen" FT /evidence="ECO:0000250" SQ SEQUENCE 177 AA; 20447 MW; 093E53E555F8E2C8 CRC64; MDYGYIHLII GPMFSGKSTE LIRIVKRYQI AQYKCCVVKY LKDIRYGNSV YTHDNNHVSA ISTTLLYDVV DKIMNFDIIG IDEGQFFKDI VSFSENMANM GKIIIIAALD STFQRKEFND ILKLIPLSEK VTKLNAVCME CYKDAAFSKR ITKEKEIELI GGKEKYKSVC RKCYFLE //