ID   NCK1_HUMAN     STANDARD;      PRT;   377 AA.
AC   P16333;
DT   01-AUG-1990 (Rel. 15, Created)
DT   01-AUG-1990 (Rel. 15, Last sequence update)
DT   10-MAY-2005 (Rel. 47, Last annotation update)
DE   Cytoplasmic protein NCK1 (NCK adaptor protein 1) (SH2/SH3 adaptor
DE   protein NCK-alpha).
GN   Name=NCK1; Synonyms=NCK;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=90192089; PubMed=2107526;
RA   Lehmann J.M., Riethmueller G., Johnson J.P.;
RT   "Nck, a melanoma cDNA encoding a cytoplasmic protein consisting of the
RT   src homology units SH2 and SH3.";
RL   Nucleic Acids Res. 18:1048-1048(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Uterus;
RX   MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899;
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA   Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA   Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA   Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA   Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA   Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA   Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA   Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA   Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA   Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA   Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA   Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA   Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA   Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA   Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA   Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human
RT   and mouse cDNA sequences.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN   [3]
RP   PHOSPHORYLATION, AND PARTIAL PROTEIN SEQUENCE.
RX   MEDLINE=93078783; PubMed=1333046;
RA   Park D., Rhee S.G.;
RT   "Phosphorylation of Nck in response to a variety of receptors, phorbol
RT   myristate acetate, and cyclic AMP.";
RL   Mol. Cell. Biol. 12:5816-5823(1992).
RN   [4]
RP   PHOSPHORYLATION.
RX   MEDLINE=93078786; PubMed=1448108;
RA   Meisenhelder J., Hunter T.;
RT   "The SH2/SH3 domain-containing protein Nck is recognized by certain
RT   anti-phospholipase C-gamma 1 monoclonal antibodies, and its
RT   phosphorylation on tyrosine is stimulated by platelet-derived growth
RT   factor and epidermal growth factor treatment.";
RL   Mol. Cell. Biol. 12:5843-5856(1992).
CC   -!- FUNCTION: Adapter protein which associates with tyrosine-
CC       phosphorylated growth factor receptors or their cellular
CC       substrates.
CC   -!- INTERACTION:
CC       Q8IZP0:ABI1; NbExp=1; IntAct=EBI-389883, EBI-375446;
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic.
CC   -!- PTM: Phosphorylated on Ser and Tyr residues.
CC   -!- SIMILARITY: Contains 1 SH2 domain.
CC   -!- SIMILARITY: Contains 3 SH3 domains.
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DR   EMBL; X17576; CAA35599.1; -; mRNA.
DR   EMBL; BC006403; AAH06403.1; -; mRNA.
DR   PIR; S08636; S08636.
DR   HSSP; P19174; 2HSP.
DR   SMR; P16333; 190-254.
DR   IntAct; P16333; -.
DR   Ensembl; ENSG00000158092; Homo sapiens.
DR   Genew; HGNC:7664; NCK1.
DR   H-InvDB; HIX0003707; -.
DR   MIM; 600508; -.
DR   GO; GO:0005737; C:cytoplasm; NAS.
DR   GO; GO:0008093; F:cytoskeletal adaptor activity; NAS.
DR   GO; GO:0005102; F:receptor binding; IPI.
DR   GO; GO:0030159; F:receptor signaling complex scaffold activity; NAS.
DR   GO; GO:0030838; P:positive regulation of actin filament polym...; IMP.
DR   GO; GO:0042102; P:positive regulation of T cell proliferation; IMP.
DR   GO; GO:0007172; P:signal complex formation; NAS.
DR   GO; GO:0042110; P:T cell activation; IMP.
DR   InterPro; IPR000108; Neu_cyt_fact_2.
DR   InterPro; IPR000980; SH2.
DR   InterPro; IPR001452; SH3.
DR   Pfam; PF00017; SH2; 1.
DR   Pfam; PF00018; SH3; 3.
DR   PRINTS; PR00401; SH2DOMAIN.
DR   PRINTS; PR00452; SH3DOMAIN.
DR   ProDom; PD000093; SH2; 1.
DR   ProDom; PD000066; SH3; 3.
DR   SMART; SM00252; SH2; 1.
DR   SMART; SM00326; SH3; 3.
DR   PROSITE; PS50001; SH2; 1.
DR   PROSITE; PS50002; SH3; 3.
KW   Direct protein sequencing; Phosphorylation; Repeat; SH2 domain;
KW   SH3 domain.
FT   DOMAIN        2     61       SH3 1.
FT   DOMAIN      115    165       SH3 2.
FT   DOMAIN      190    252       SH3 3.
FT   DOMAIN      282    376       SH2.
SQ   SEQUENCE   377 AA;  42864 MW;  554E9B1A936AEF30 CRC64;
     MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN
     SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE
     REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA
     AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVI EKPENDPEWW KCRKINGMVG
     LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG
     HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP
     IFTSEQGEKL YLVKHLS
//