ID TCDE_CLODI Reviewed; 166 AA. AC P16153; DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1996, sequence version 2. DT 07-OCT-2020, entry version 84. DE RecName: Full=Holin-like protein TcdE; GN Name=tcdE {ECO:0000303|PubMed:22685398}; GN Synonyms=utxA {ECO:0000303|PubMed:1603068}; OS Clostridioides difficile (Peptoclostridium difficile). OC Bacteria; Firmicutes; Clostridia; Clostridiales; Peptostreptococcaceae; OC Clostridioides. OX NCBI_TaxID=1496; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 4325 / VPI 10463; RX PubMed=1603068; DOI=10.1007/bf00587587; RA von Eichel-Streiber C., Laufenberg-Feldmann R., Sartingen S., Schulze J., RA Sauerborn M.; RT "Comparative sequence analysis of the Clostridium difficile toxins A and RT B."; RL Mol. Gen. Genet. 233:260-268(1992). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 4325 / VPI 10463; RX PubMed=2105276; RA Dove C.H., Wang S.-Z., Price S.B., Phelps C.J., Lyerly D.M., Wilkins T.D., RA Johnson J.L.; RT "Molecular characterization of the Clostridium difficile toxin A gene."; RL Infect. Immun. 58:480-488(1990). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 4325 / VPI 10463; RA von Eichel-Streiber C.; RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-166. RC STRAIN=ATCC 4325 / VPI 10463; RX PubMed=2109310; DOI=10.1093/nar/18.6.1629; RA Sauerborn M., von Eichel-Streiber C.; RT "Nucleotide sequence of Clostridium difficile toxin A."; RL Nucleic Acids Res. 18:1629-1630(1990). RN [5] RP FUNCTION, ALTERNATIVE INITIATION (ISOFORMS 1; 2 AND 3), SUBUNIT, RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE. RX PubMed=22685398; DOI=10.1371/journal.ppat.1002727; RA Govind R., Dupuy B.; RT "Secretion of Clostridium difficile toxins A and B requires the holin-like RT protein TcdE."; RL PLoS Pathog. 8:e1002727-e1002727(2012). CC -!- FUNCTION: [Isoform 1]: Holin-like protein required for secretion of CC toxins A and B (TcdA and TcdB) (PubMed:22685398). Facilitates the CC release of toxins to the extracellular environment without causing the CC bacterial cell lysis (PubMed:22685398). {ECO:0000269|PubMed:22685398}. CC -!- FUNCTION: [Isoform 2]: Has weak activity, suggesting that it may act as CC a antiholin when multiple forms are produced. CC {ECO:0000305|PubMed:22685398}. CC -!- FUNCTION: [Isoform 3]: Has weak activity, suggesting that it may act as CC a antiholin when multiple forms are produced. CC {ECO:0000305|PubMed:22685398}. CC -!- SUBUNIT: Homomultimer. {ECO:0000269|PubMed:22685398}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22685398}; CC Multi-pass membrane protein {ECO:0000255}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative initiation; Named isoforms=3; CC Name=1; Synonyms=Long {ECO:0000303|PubMed:1603068}; CC IsoId=P16153-1; Sequence=Displayed; CC Name=2; Synonyms=Short {ECO:0000303|PubMed:1603068}; CC IsoId=P16153-2; Sequence=VSP_018824; CC Name=3; CC IsoId=P16153-3; Sequence=VSP_060769; CC -!- DISRUPTION PHENOTYPE: Cells grow normally but accumulate a strongly CC reduced amount of toxins TcdA and TcdB in the medium. CC {ECO:0000269|PubMed:22685398}. CC -!- SIMILARITY: Belongs to the bacteriophage holin family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X60984; CAA43300.1; -; Genomic_DNA. DR EMBL; X60984; CAA43301.1; -; Genomic_DNA. DR EMBL; M30308; AAA83532.1; -; Genomic_DNA. DR EMBL; M30308; AAA83533.1; -; Genomic_DNA. DR EMBL; X92982; CAA63563.1; -; Genomic_DNA. DR EMBL; X51797; CAA36093.1; -; Genomic_DNA. DR PIR; B37052; B37052. DR RefSeq; WP_003434540.1; NZ_VINH01000002.1. [P16153-1] DR RefSeq; WP_018112585.1; NZ_BIWI01000430.1. DR TCDB; 1.E.19.3.1; the clostridium difficile tcde holin (tcde holin) family. DR GeneID; 31352217; -. DR GO; GO:0034291; F:canonical holin activity; IDA:CACAO. DR GO; GO:1901998; P:toxin transport; IMP:CACAO. DR InterPro; IPR006480; Phage_holin_4_1. DR Pfam; PF05105; Phage_holin_4_1; 1. DR TIGRFAMs; TIGR01593; holin_tox_secr; 1. PE 1: Evidence at protein level; KW Alternative initiation; Cell membrane; Membrane; Protein transport; KW Transmembrane; Transmembrane helix; Transport. FT CHAIN 1..166 FT /note="Holin-like protein TcdE" FT /id="PRO_0000022643" FT TRANSMEM 15..35 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 36..56 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 77..97 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 111..131 FT /note="Helical" FT /evidence="ECO:0000255" FT VAR_SEQ 1..26 FT /note="Missing (in isoform 2)" FT /evidence="ECO:0000305" FT /id="VSP_018824" FT VAR_SEQ 1..24 FT /note="Missing (in isoform 3)" FT /evidence="ECO:0000305" FT /id="VSP_060769" SQ SEQUENCE 166 AA; 18800 MW; 1671FAE1884C7EE2 CRC64; MHSSSPFYIS NGNKIFFYIN LGGVMNMTIS FLSEHIFIKL VILTISFDTL LGCLSAIKSR KFNSSFGIDG GIRKVAMIAC IFFLSVVDIL TKFNFLFMLP QDCINFLRLK HLGISEFFSI LFILYESVSI LKNMCLCGLP VPKRLKEKIA ILLDAMTDEM NAKDEK //