ID   PSB2_SYNY3     STANDARD;      PRT;   360 AA.
AC   P16033;
DT   01-APR-1990 (Rel. 14, Created)
DT   01-APR-1990 (Rel. 14, Last sequence update)
DT   13-SEP-2005 (Rel. 48, Last annotation update)
DE   Photosystem Q(B) protein (32 kDa thylakoid membrane protein)
DE   (Photosystem II protein D1).
GN   Name=psbA2; Synonyms=psbA-2; OrderedLocusNames=slr1311;
GN   and
GN   Name=psbA3; Synonyms=psbA-3; OrderedLocusNames=sll1867;
OS   Synechocystis sp. (strain PCC 6803).
OC   Bacteria; Cyanobacteria; Chroococcales; Synechocystis.
OX   NCBI_TaxID=1148;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=91067488; PubMed=2123543;
RA   Metz J., Nixon P., Diner B.;
RT   "Nucleotide sequence of the psbA3 gene from the cyanobacterium
RT   Synechocystis PCC 6803.";
RL   Nucleic Acids Res. 18:6715-6715(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=89282415; PubMed=2499875;
RA   Ravnikar P.D., Debus R., Sevrinck J., Saetaert P., McIntosh L.;
RT   "Nucleotide sequence of a second psbA gene from the unicellular
RT   cyanobacterium Synechocystis 6803.";
RL   Nucleic Acids Res. 17:3991-3991(1989).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=97061201; PubMed=8905231;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T.,
RA   Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S.,
RA   Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M.,
RA   Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the
RT   entire genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
RN   [4]
RP   MUTAGENESIS FOR HERBICIDE RESISTANCE.
RX   MEDLINE=92361253; PubMed=1498597; DOI=10.1105/tpc.4.3.273;
RA   Ohad N., Hirschberg J.;
RT   "Mutations in the D1 subunit of photosystem II distinguish between
RT   quinone and herbicide binding sites.";
RL   Plant Cell 4:273-282(1992).
RN   [5]
RP   MUTAGENESIS FOR HERBICIDE RESISTANCE.
RX   MEDLINE=94347789; PubMed=8068689;
RA   Kless H., Oren-Shamir M., Malkin S., McIntosh L., Edelman M.;
RT   "The D-E region of the D1 protein is involved in multiple quinone and
RT   herbicide interactions in photosystem II.";
RL   Biochemistry 33:10501-10507(1994).
CC   -!- FUNCTION: This is one of the two reaction center proteins of the
CC       photosystem II.
CC   -!- SUBCELLULAR LOCATION: Integral membrane protein. Thylakoid
CC       membrane.
CC   -!- MISCELLANEOUS: Herbicides such as atrazine, BNT, diuron or ioxynil
CC       bind to Q(B) and blocks the electron transport.
CC   -!- MISCELLANEOUS: Cyanobacteria are generally known to contain 2 to 3
CC       copies of the psbA gene.
CC   -!- SIMILARITY: Belongs to the reaction center pufL/M/psbA/D family.
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DR   EMBL; X56000; CAA39472.1; -; Genomic_DNA.
DR   EMBL; X13547; CAA31899.1; -; Genomic_DNA.
DR   EMBL; BA000022; BAA18230.1; -; Genomic_DNA.
DR   EMBL; BA000022; BAA16586.1; -; Genomic_DNA.
DR   PIR; S13112; F2YB16.
DR   HSSP; P02955; 1DOP.
DR   SMR; P16033; 12-344.
DR   InterPro; IPR005867; Photo_DI.
DR   InterPro; IPR000484; Photo_RC.
DR   Pfam; PF00124; Photo_RC; 1.
DR   PRINTS; PR00256; REACTNCENTRE.
DR   ProDom; PD000551; Photo_RC; 1.
DR   TIGRFAMs; TIGR01151; psbA; 1.
DR   PROSITE; PS00244; REACTION_CENTER; 1.
KW   Complete proteome; Electron transport; Herbicide resistance; Iron;
KW   Metal-binding; Multigene family; Photosynthesis; Photosystem II;
KW   Thylakoid; Transmembrane; Transport.
FT   TRANSMEM     36     56       Potential.
FT   TRANSMEM    109    129       Potential.
FT   TRANSMEM    141    164       Potential.
FT   TRANSMEM    192    218       Potential.
FT   TRANSMEM    269    289       Potential.
FT   METAL       215    215       Iron (non heme).
FT   METAL       272    272       Iron (non heme).
FT   MUTAGEN     221    222       SS->LA: Strong herbicide resistance.
FT   MUTAGEN     227    228       TT->AA: Herbicide resistance.
FT   MUTAGEN     237    237       Y->F: Weak herbicide resistance.
FT   MUTAGEN     238    238       K->V: Weak herbicide resistance.
FT   MUTAGEN     255    255       F->W: Herbicide resistance.
FT   MUTAGEN     257    257       R->V: Herbicide resistance.
FT   MUTAGEN     264    264       S->A: Strong herbicide resistance.
FT   MUTAGEN     271    271       L->V,M,A,S: Herbicide resistance.
SQ   SEQUENCE   360 AA;  39721 MW;  2FE4088498A416C2 CRC64;
     MTTTLQQRES ASLWEQFCQW VTSTNNRIYV GWFGTLMIPT LLTATTCFII AFIAAPPVDI
     DGIREPVAGS LLYGNNIISG AVVPSSNAIG LHFYPIWEAA SLDEWLYNGG PYQLVVFHFL
     IGIFCYMGRQ WELSYRLGMR PWICVAYSAP VSAATAVFLI YPIGQGSFSD GMPLGISGTF
     NFMIVFQAEH NILMHPFHML GVAGVFGGSL FSAMHGSLVT SSLVRETTEV ESQNYGYKFG
     QEEETYNIVA AHGYFGRLIF QYASFNNSRS LHFFLGAWPV IGIWFTAMGV STMAFNLNGF
     NFNQSILDSQ GRVIGTWADV LNRANIGFEV MHERNAHNFP LDLASGEQAP VALTAPAVNG
//