ID MET8_YEAST STANDARD; PRT; 274 AA. AC P15807; DT 01-APR-1990 (Rel. 14, Created) DT 01-APR-1990 (Rel. 14, Last sequence update) DT 16-OCT-2001 (Rel. 40, Last annotation update) DE Siroheme biosynthesis protein MET8 [Includes: Precorrin-2 oxidase DE (EC 1.-.-.-); Ferrochelatase (EC 4.99.1.-)]. GN MET8 OR YBR213W OR YBR1461. OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP SEQUENCE FROM N.A. RC STRAIN=X2180-1A; RX MEDLINE=90174997; PubMed=2408020; RA Cherest H., Thomas D., Surdin-Kerjan Y.; RT "Nucleotide sequence of the MET8 gene of Saccharomyces cerevisiae."; RL Nucleic Acids Res. 18:659-659(1990). RN [2] RP SEQUENCE FROM N.A. RC STRAIN=S288C; RA Rieger M.; RL Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases. RN [3] RP FUNCTION. RX PubMed=10051442; RA Raux E., McVeigh T., Peters S.E., Leustek T., Warren M.J.; RT "The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and RT cobalamin biosynthesis."; RL Biochem. J. 338:701-708(1999). CC -!- FUNCTION: Catalyzes the conversion of precorrin-2 into siroheme. CC This reaction consist of the NAD-dependent oxidation of precorrin- CC 2 into sirohydrochlorin and its subsequent ferrochelation into CC siroheme. CC -!- PATHWAY: SIROHEME BIOSYNTHESIS. CC -!- SIMILARITY: TO S.POMBE SPAC4D7.06C. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X17271; CAA35173.1; -. DR EMBL; Z36082; CAA85177.1; -. DR PIR; S20155; S20155. DR SGD; S0000417; MET8. KW Multifunctional enzyme; Methionine biosynthesis; KW Porphyrin biosynthesis; Oxidoreductase; NAD; Lyase. SQ SEQUENCE 274 AA; 31917 MW; 0EE9A6DE8A43673E CRC64; MVKSLQLAHQ LKDKKILLIG GGEVGLTRLY KLIPTGCKLT LVSPDLHKSI IPKFGKFIQN EDQPDYREDA KRFINPNWDP TKNEIYEYIR SDFKDEYLDL EDENDAWYII MTCIPDHPES ARIYHLCKER FGKQQLVNVA DKPDLCDFYF GANLEIGDRL QILISTNGLS PRFGALVRDE IRNLFTQMGD LALEDAVVKL GELRRGIRLL APDDKDVKYR MDWARRCTDL FGIQHCHNID VKRLLDLFKV MFQEQNCSLQ FPPRERLLSE YCSS //