ID PLST_HUMAN STANDARD; PRT; 627 AA. AC P13797; DT 01-JAN-1990 (Rel. 13, Created) DT 01-NOV-1997 (Rel. 35, Last sequence update) DT 07-FEB-2006 (Rel. 49, Last annotation update) DE Plastin-3 (T-plastin). GN Name=PLS3; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE, AND PARTIAL PROTEIN SEQUENCE. RX MEDLINE=89096835; PubMed=3211125; RA Lin C.-S., Aebersold R.H., Kent S.B., Varma M., Leavitt J.; RT "Molecular cloning and characterization of plastin, a human leukocyte RT protein expressed in transformed human fibroblasts."; RL Mol. Cell. Biol. 8:4659-4668(1988). RN [2] RP SEQUENCE REVISION. RX MEDLINE=90205868; PubMed=2378651; RA Lin C.-S., Aebersold R.H., Leavitt J.; RT "Correction of the N-terminal sequences of the human plastin isoforms RT by using anchored polymerase chain reaction: identification of a RT potential calcium-binding domain."; RL Mol. Cell. Biol. 10:1818-1821(1990). RN [3] RP NUCLEOTIDE SEQUENCE OF 585-627. RX MEDLINE=93155095; PubMed=8428952; RA Lin C.-S., Park T., Chen Z.P., Leavitt J.; RT "Human plastin genes. Comparative gene structure, chromosome location, RT and differential expression in normal and neoplastic cells."; RL J. Biol. Chem. 268:2781-2792(1993). RN [4] RP X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 118-372. RX MEDLINE=97448672; PubMed=9302997; RA Goldsmith S.C., Pokala N., Shen W., Fedorov A.A., Matsudaira P., RA Almo S.C.; RT "The structure of an actin-crosslinking domain from human fimbrin."; RL Nat. Struct. Biol. 4:708-712(1997). CC -!- FUNCTION: Actin-bundling protein found in intestinal microvilli, CC hair cell stereocilia, and fibroblast filopodia. CC -!- SUBUNIT: Monomer. CC -!- INTERACTION: CC Q99700:ATXN2; NbExp=1; IntAct=EBI-698052, EBI-697691; CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- TISSUE SPECIFICITY: Expressed in a variety of organs, including CC muscle, brain, uterus, and esophagus. CC -!- PTM: Phosphorylated. CC -!- SIMILARITY: Contains 2 actin-binding domains. CC -!- SIMILARITY: Contains 4 CH (calponin-homology) domains. CC -!- SIMILARITY: Contains 2 EF-hand domains. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M22299; AAB02844.1; ALT_INIT; mRNA. DR EMBL; M34427; AAA36759.1; ALT_INIT; mRNA. DR EMBL; L05491; AAA61214.1; -; Genomic_DNA. DR PIR; A34789; A34789. DR PDB; 1AOA; X-ray; @=98-372. DR PDB; 1WJO; NMR; A=517-627. DR IntAct; P13797; -. DR Ensembl; ENSG00000102024; Homo sapiens. DR HGNC; HGNC:9091; PLS3. DR MIM; 300131; gene. DR LinkHub; P13797; -. DR InterPro; IPR001589; Actnin_actin_bd. DR InterPro; IPR001715; Calponin_act_bd. DR InterPro; IPR011992; EF-Hand_type. DR InterPro; IPR002048; EF_hand_Ca_bd. DR Pfam; PF00307; CH; 4. DR Pfam; PF00036; efhand; 2. DR ProDom; PD000012; EF-hand; 1. DR SMART; SM00033; CH; 4. DR SMART; SM00054; EFh; 2. DR PROSITE; PS00019; ACTININ_1; 2. DR PROSITE; PS00020; ACTININ_2; 2. DR PROSITE; PS50021; CH; 4. DR PROSITE; PS00018; EF_HAND_1; 2. DR PROSITE; PS50222; EF_HAND_2; 2. KW 3D-structure; Actin-binding; Calcium; Direct protein sequencing; KW Phosphorylation; Repeat. FT DOMAIN 9 44 EF-hand 1. FT DOMAIN 49 84 EF-hand 2. FT DOMAIN 106 379 Actin-binding 1. FT DOMAIN 120 236 CH 1. FT DOMAIN 264 375 CH 2. FT DOMAIN 380 624 Actin-binding 2. FT DOMAIN 394 503 CH 3. FT DOMAIN 515 624 CH 4. FT CA_BIND 22 33 1 (By similarity). FT CA_BIND 62 73 2 (By similarity). FT HELIX 120 133 FT TURN 134 136 FT TURN 138 139 FT TURN 141 143 FT TURN 148 151 FT HELIX 152 156 FT HELIX 157 159 FT HELIX 161 170 FT TURN 172 173 FT HELIX 177 179 FT HELIX 187 203 FT TURN 204 205 FT TURN 209 210 FT HELIX 213 217 FT TURN 218 219 FT HELIX 221 241 FT TURN 245 246 FT HELIX 258 262 FT TURN 263 263 FT HELIX 266 280 FT TURN 281 282 FT TURN 291 295 FT HELIX 297 306 FT TURN 323 326 FT HELIX 330 341 FT TURN 342 345 FT HELIX 352 356 FT TURN 357 358 FT HELIX 360 371 SQ SEQUENCE 627 AA; 70436 MW; 52AC3E044D198D19 CRC64; MATTQISKDE LDELKEAFAK VDLNSNGFIC DYELHELFKE ANMPLPGYKV REIIQKLMLD GDRNKDGKIS FDEFVYIFQE VKSSDIAKTF RKAINRKEGI CALGGTSELS SEGTQHSYSE EEKYAFVNWI NKALENDPDC RHVIPMNPNT DDLFKAVGDG IVLCKMINLS VPDTIDERAI NKKKLTPFII QENLNLALNS ASAIGCHVVN IGAEDLRAGK PHLVLGLLWQ IIKIGLFADI ELSRNEALAA LLRDGETLEE LMKLSPEELL LRWANFHLEN SGWQKINNFS ADIKDSKAYF HLLNQIAPKG QKEGEPRIDI NMSGFNETDD LKRAESMLQQ ADKLGCRQFV TPADVVSGNP KLNLAFVANL FNKYPALTKP ENQDIDWTLL EGETREERTF RNWMNSLGVN PHVNHLYADL QDALVILQLY ERIKVPVDWS KVNKPPYPKL GANMKKLENC NYAVELGKHP AKFSLVGIGG QDLNDGNQTL TLALVWQLMR RYTLNVLEDL GDGQKANDDI IVNWVNRTLS EAGKSTSIQS FKDKTISSSL AVVDLIDAIQ PGCINYDLVK SGNLTEDDKH NNAKYAVSMA RRIGARVYAL PEDLVEVKPK MVMTVFACLM GRGMKRV //