ID ESM4_DROME Reviewed; 152 AA. AC P13095; Q56L08; Q56L11; Q56L12; Q56L19; Q56L29; Q56L35; Q56L44; Q56L45; AC Q56L64; Q56L67; Q56L68; Q56L69; Q56L92; Q5S478; Q5S4B4; Q5S4J8; Q9VBI9; DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot. DT 01-JAN-1990, sequence version 1. DT 10-FEB-2021, entry version 136. DE RecName: Full=Enhancer of split m4 protein {ECO:0000303|PubMed:2540957}; DE Short=E(spl)m4 {ECO:0000303|PubMed:2540957}; GN Name=E(spl)m4-BFM {ECO:0000312|FlyBase:FBgn0002629}; GN Synonyms=m4 {ECO:0000303|PubMed:2540957}; GN ORFNames=CG6099 {ECO:0000312|FlyBase:FBgn0002629}; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=2540957; DOI=10.1002/j.1460-2075.1989.tb03365.x; RA Klaembt C., Knust E., Tietze K., Campos-Ortega J.A.; RT "Closely related transcripts encoded by the neurogenic gene complex RT enhancer of split of Drosophila melanogaster."; RL EMBO J. 8:203-210(1989). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ALA-11 AND LYS-82. RC STRAIN=NVIII-1, NVIII-18, NVIII-2, NVIII-22, NVIII-24, NVIII-28, NVIII-41, RC NVIII-42, NVIII-46, NVIII-5, NVIII-9, NVIII-m11, NVIII-m12, NVIII-m13, RC NVIII-m15, and NVIII-m19; RX PubMed=15537803; DOI=10.1093/molbev/msi046; RA Macdonald S.J., Long A.D.; RT "Identifying signatures of selection at the enhancer of split neurogenic RT gene complex in Drosophila."; RL Mol. Biol. Evol. 22:607-619(2005). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., RA Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [4] RP GENOME REANNOTATION. RC STRAIN=Berkeley; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [5] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125, AND VARIANTS ALA-11 AND PRO-63. RC STRAIN=Nv2001_m0564, Nv2001_m0565, Nv2001_m0566, Nv2001_m0567, RC Nv2001_m0568, Nv2001_m0569, Nv2001_m0570, Nv2001_m0571, Nv2001_m0572, RC Nv2001_m0573, Nv2001_m0574, Nv2001_m0575, Nv2001_m0576, Nv2001_m0577, RC Nv2001_m0578, Nv2001_m0579, Nv2001_m0580, Nv2001_m0581, Nv2001_m0582, RC Nv2001_m0583, Nv2001_m0584, Nv2001_m0585, Nv2001_m0586, Nv2001_m0587, RC Nv2001_m0588, Nv2001_m0589, Nv2001_m0590, Nv2001_m0591, Nv2001_m0592, RC Nv2001_m0593, Nv2001_m0594, Nv2001_m0595, Nv2001_m0596, Nv2001_m0597, RC Nv2001_m0598, Nv2001_m0599, Nv2001_m0600, Nv2001_m0601, Nv2001_m0602, RC Nv2001_m0603, Nv2001_m0604, Nv2001_m0605, Nv2001_m0606, Nv2001_m0607, RC Nv2001_m0608, Nv2001_m0609, Nv2001_m0610, Nv2001_m0611, Nv2001_m0612, RC Nv2001_m0613, Nv2001_m0614, Nv2001_m0615, Nv2001_m0616, Nv2001_m0617, RC Nv2001_m0618, Nv2001_m0619, Nv2001_m0620, Nv2001_m0622, Nv2001_m0623, RC Nv2001_m0624, Nv2001_m0625, Nv2001_m0626, Nv2001_m0627, Nv2001_m0628, RC Nv2001_m0629, Nv2001_m0630, Nv2001_m0631, Nv2001_m0632, Nv2001_m0633, RC Nv2001_m0634, Nv2001_m0635, Nv2001_m0636, Nv2001_m0637, Nv2001_m0638, RC Nv2001_m0639, Nv2001_m0640, Nv2001_m0641, Nv2001_m0642, Nv2001_m0643, RC Nv2001_m0644, Nv2001_m0645, Nv2001_m0646, Nv2001_m0647, Nv2001_m0648, RC Nv2001_m0649, Nv2001_m0650, Nv2001_m0651, Nv2001_m0652, Nv2001_m0653, RC Nv2001_m0654, Nv2001_m0655, Nv2001_m0656, and Nv2001_m0657; RX PubMed=16356268; DOI=10.1186/gb-2005-6-12-r105; RA Macdonald S.J., Pastinen T., Genissel A., Cornforth T.W., Long A.D.; RT "A low-cost open-source SNP genotyping platform for association mapping RT applications."; RL Genome Biol. 6:R105.1-R105.11(2005). CC -!- FUNCTION: Part of the Notch signaling pathway. CC -!- DEVELOPMENTAL STAGE: Expressed at the time when separation of neural CC and epidermal precursors cells occurs. Mesectodermal expression appears CC shortly before the onset of gastrulation. In imaginal disks, expression CC is seen primarily within presumptive proneural clusters of eye- CC antennal, wing and leg disks. CC -!- SIMILARITY: Belongs to the M4-like protein family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X16551; CAA34551.1; -; Genomic_DNA. DR EMBL; AY779906; AAV59055.1; -; Genomic_DNA. DR EMBL; AY779907; AAV59067.1; -; Genomic_DNA. DR EMBL; AY779908; AAV59079.1; -; Genomic_DNA. DR EMBL; AY779909; AAV59091.1; -; Genomic_DNA. DR EMBL; AY779910; AAV59103.1; -; Genomic_DNA. DR EMBL; AY779911; AAV59115.1; -; Genomic_DNA. DR EMBL; AY779912; AAV59127.1; -; Genomic_DNA. DR EMBL; AY779913; AAV59139.1; -; Genomic_DNA. DR EMBL; AY779914; AAV59151.1; -; Genomic_DNA. DR EMBL; AY779915; AAV59163.1; -; Genomic_DNA. DR EMBL; AY779916; AAV59175.1; -; Genomic_DNA. DR EMBL; AY779917; AAV59187.1; -; Genomic_DNA. DR EMBL; AY779918; AAV59199.1; -; Genomic_DNA. DR EMBL; AY779919; AAV59211.1; -; Genomic_DNA. DR EMBL; AY779920; AAV59223.1; -; Genomic_DNA. DR EMBL; AY779921; AAV59235.1; -; Genomic_DNA. DR EMBL; AE014297; AAF56551.1; -; Genomic_DNA. DR EMBL; AY906350; AAX60696.1; -; Genomic_DNA. DR EMBL; AY906351; AAX60697.1; -; Genomic_DNA. DR EMBL; AY906352; AAX60698.1; -; Genomic_DNA. DR EMBL; AY906353; AAX60699.1; -; Genomic_DNA. DR EMBL; AY906354; AAX60700.1; -; Genomic_DNA. DR EMBL; AY906355; AAX60701.1; -; Genomic_DNA. DR EMBL; AY906356; AAX60702.1; -; Genomic_DNA. DR EMBL; AY906357; AAX60703.1; -; Genomic_DNA. DR EMBL; AY906358; AAX60704.1; -; Genomic_DNA. DR EMBL; AY906359; AAX60705.1; -; Genomic_DNA. DR EMBL; AY906360; AAX60706.1; -; Genomic_DNA. DR EMBL; AY906361; AAX60707.1; -; Genomic_DNA. DR EMBL; AY906362; AAX60708.1; -; Genomic_DNA. DR EMBL; AY906363; AAX60709.1; -; Genomic_DNA. DR EMBL; AY906364; AAX60710.1; -; Genomic_DNA. DR EMBL; AY906365; AAX60711.1; -; Genomic_DNA. DR EMBL; AY906366; AAX60712.1; -; Genomic_DNA. DR EMBL; AY906367; AAX60713.1; -; Genomic_DNA. DR EMBL; AY906368; AAX60714.1; -; Genomic_DNA. DR EMBL; AY906369; AAX60715.1; -; Genomic_DNA. DR EMBL; AY906370; AAX60716.1; -; Genomic_DNA. DR EMBL; AY906371; AAX60717.1; -; Genomic_DNA. DR EMBL; AY906372; AAX60718.1; -; Genomic_DNA. DR EMBL; AY906373; AAX60719.1; -; Genomic_DNA. DR EMBL; AY906374; AAX60720.1; -; Genomic_DNA. DR EMBL; AY906375; AAX60721.1; -; Genomic_DNA. DR EMBL; AY906376; AAX60722.1; -; Genomic_DNA. DR EMBL; AY906377; AAX60723.1; -; Genomic_DNA. DR EMBL; AY906378; AAX60724.1; -; Genomic_DNA. DR EMBL; AY906379; AAX60725.1; -; Genomic_DNA. DR EMBL; AY906380; AAX60726.1; -; Genomic_DNA. DR EMBL; AY906381; AAX60727.1; -; Genomic_DNA. DR EMBL; AY906382; AAX60728.1; -; Genomic_DNA. DR EMBL; AY906383; AAX60729.1; -; Genomic_DNA. DR EMBL; AY906384; AAX60730.1; -; Genomic_DNA. DR EMBL; AY906385; AAX60731.1; -; Genomic_DNA. DR EMBL; AY906386; AAX60732.1; -; Genomic_DNA. DR EMBL; AY906387; AAX60733.1; -; Genomic_DNA. DR EMBL; AY906388; AAX60734.1; -; Genomic_DNA. DR EMBL; AY906389; AAX60735.1; -; Genomic_DNA. DR EMBL; AY906390; AAX60736.1; -; Genomic_DNA. DR EMBL; AY906391; AAX60737.1; -; Genomic_DNA. DR EMBL; AY906392; AAX60738.1; -; Genomic_DNA. DR EMBL; AY906393; AAX60739.1; -; Genomic_DNA. DR EMBL; AY906394; AAX60740.1; -; Genomic_DNA. DR EMBL; AY906395; AAX60741.1; -; Genomic_DNA. DR EMBL; AY906396; AAX60742.1; -; Genomic_DNA. DR EMBL; AY906397; AAX60743.1; -; Genomic_DNA. DR EMBL; AY906398; AAX60744.1; -; Genomic_DNA. DR EMBL; AY906399; AAX60745.1; -; Genomic_DNA. DR EMBL; AY906400; AAX60746.1; -; Genomic_DNA. DR EMBL; AY906401; AAX60747.1; -; Genomic_DNA. DR EMBL; AY906402; AAX60748.1; -; Genomic_DNA. DR EMBL; AY906403; AAX60749.1; -; Genomic_DNA. DR EMBL; AY906404; AAX60750.1; -; Genomic_DNA. DR EMBL; AY906405; AAX60751.1; -; Genomic_DNA. DR EMBL; AY906406; AAX60752.1; -; Genomic_DNA. DR EMBL; AY906407; AAX60753.1; -; Genomic_DNA. DR EMBL; AY906408; AAX60754.1; -; Genomic_DNA. DR EMBL; AY906409; AAX60755.1; -; Genomic_DNA. DR EMBL; AY906410; AAX60756.1; -; Genomic_DNA. DR EMBL; AY906411; AAX60757.1; -; Genomic_DNA. DR EMBL; AY906412; AAX60758.1; -; Genomic_DNA. DR EMBL; AY906413; AAX60759.1; -; Genomic_DNA. DR EMBL; AY906414; AAX60760.1; -; Genomic_DNA. DR EMBL; AY906415; AAX60761.1; -; Genomic_DNA. DR EMBL; AY906416; AAX60762.1; -; Genomic_DNA. DR EMBL; AY906417; AAX60763.1; -; Genomic_DNA. DR EMBL; AY906418; AAX60764.1; -; Genomic_DNA. DR EMBL; AY906419; AAX60765.1; -; Genomic_DNA. DR EMBL; AY906420; AAX60766.1; -; Genomic_DNA. DR EMBL; AY906421; AAX60767.1; -; Genomic_DNA. DR EMBL; AY906422; AAX60768.1; -; Genomic_DNA. DR EMBL; AY906423; AAX60769.1; -; Genomic_DNA. DR EMBL; AY906424; AAX60770.1; -; Genomic_DNA. DR EMBL; AY906425; AAX60771.1; -; Genomic_DNA. DR EMBL; AY906426; AAX60772.1; -; Genomic_DNA. DR EMBL; AY906427; AAX60773.1; -; Genomic_DNA. DR EMBL; AY906428; AAX60774.1; -; Genomic_DNA. DR EMBL; AY906429; AAX60775.1; -; Genomic_DNA. DR EMBL; AY906430; AAX60776.1; -; Genomic_DNA. DR EMBL; AY906431; AAX60777.1; -; Genomic_DNA. DR EMBL; AY906432; AAX60778.1; -; Genomic_DNA. DR EMBL; AY906433; AAX60779.1; -; Genomic_DNA. DR EMBL; AY906434; AAX60780.1; -; Genomic_DNA. DR EMBL; AY906435; AAX60781.1; -; Genomic_DNA. DR EMBL; AY906436; AAX60782.1; -; Genomic_DNA. DR EMBL; AY906437; AAX60783.1; -; Genomic_DNA. DR EMBL; AY906438; AAX60784.1; -; Genomic_DNA. DR EMBL; AY906439; AAX60785.1; -; Genomic_DNA. DR EMBL; AY906440; AAX60786.1; -; Genomic_DNA. DR EMBL; AY906441; AAX60787.1; -; Genomic_DNA. DR EMBL; AY906442; AAX60788.1; -; Genomic_DNA. DR PIR; S03630; S03630. DR RefSeq; NP_524510.1; NM_079786.3. DR BioGRID; 68057; 9. DR DIP; DIP-23216N; -. DR IntAct; P13095; 2. DR STRING; 7227.FBpp0084353; -. DR PaxDb; P13095; -. DR EnsemblMetazoa; FBtr0084980; FBpp0084353; FBgn0002629. DR GeneID; 43157; -. DR KEGG; dme:Dmel_CG6099; -. DR CTD; 43157; -. DR FlyBase; FBgn0002629; E(spl)m4-BFM. DR eggNOG; ENOG502T7ZT; Eukaryota. DR GeneTree; ENSGT00900000142304; -. DR HOGENOM; CLU_1688576_0_0_1; -. DR InParanoid; P13095; -. DR OMA; EDYEFEQ; -. DR OrthoDB; 1480488at2759; -. DR PhylomeDB; P13095; -. DR BioGRID-ORCS; 43157; 0 hits in 1 CRISPR screen. DR GenomeRNAi; 43157; -. DR PRO; PR:P13095; -. DR Proteomes; UP000000803; Chromosome 3R. DR Bgee; FBgn0002629; Expressed in embryo and 30 other tissues. DR ExpressionAtlas; P13095; baseline and differential. DR Genevisible; P13095; DM. DR GO; GO:0031625; F:ubiquitin protein ligase binding; IPI:FlyBase. DR GO; GO:0001708; P:cell fate specification; IMP:FlyBase. DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IMP:FlyBase. DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW. DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW. DR GO; GO:0007423; P:sensory organ development; IMP:FlyBase. DR InterPro; IPR029686; Malpha/m4/m2. DR PANTHER; PTHR12254; PTHR12254; 1. DR Pfam; PF15952; ESM4; 1. PE 2: Evidence at transcript level; KW Coiled coil; Developmental protein; Differentiation; Neurogenesis; KW Notch signaling pathway; Reference proteome. FT CHAIN 1..152 FT /note="Enhancer of split m4 protein" FT /id="PRO_0000087059" FT COILED 23..61 FT /evidence="ECO:0000255" FT VARIANT 11 FT /note="T -> A (in strain: Nv2001_m0595 and NVIII-m11)" FT /evidence="ECO:0000269|PubMed:15537803, FT ECO:0000269|PubMed:16356268" FT VARIANT 63 FT /note="R -> P (in strain: Nv2001_m0592)" FT /evidence="ECO:0000269|PubMed:16356268" FT VARIANT 82 FT /note="E -> K (in strain: NVIII-41)" FT /evidence="ECO:0000269|PubMed:15537803" SQ SEQUENCE 152 AA; 17250 MW; D7F642838BD2DA0C CRC64; MCQNKINTNN TMTIKSNKKL SYSVKKLLQK IFKQQQRVEE EQNLKNALKA NSLESLESME NSRNADLESA SICASLESCE NEANERLSQS CEIEDYDFEQ LPTVPVHFVR TAHGTFFWTA VSDLPADNDL VEPLYCSTSN AIAIPQDRWV QA //