ID DMA_BPT2 Reviewed; 259 AA. AC P12427; DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1989, sequence version 1. DT 20-FEB-2007, entry version 40. DE DNA adenine methylase (EC 2.1.1.72) (Deoxyadenosyl-methyltransferase) DE (M.EcoT2Dam). GN Name=DAM; OS Bacteriophage T2. OC Viruses; dsDNA viruses, no RNA stage; Caudovirales; Myoviridae; OC T4-like viruses. OX NCBI_TaxID=10664; OH NCBI_TaxID=562; Escherichia coli. RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=89033901; PubMed=3053648; RA Miner Z., Hattman S.; RT "Molecular cloning, sequencing, and mapping of the bacteriophage T2 RT dam gene."; RL J. Bacteriol. 170:5177-5184(1988). RN [2] RP MUTAGENESIS. RX MEDLINE=90045940; PubMed=2510127; DOI=10.1093/nar/17.20.8149; RA Miner Z., Schlagman S.L., Hattman S.; RT "Single amino acid changes that alter the DNA sequence specificity of RT the DNA-[N6-adenine] methyltransferase (Dam) of bacteriophage T4."; RL Nucleic Acids Res. 17:8149-8157(1989). CC -!- FUNCTION: This methylase recognizes the double-stranded sequence CC GATC, causes specific methylation on A-2 on both strands. CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA adenine = S- CC adenosyl-L-homocysteine + DNA 6-methylaminopurine. CC -!- SIMILARITY: Belongs to the N4/N6-methyltransferase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M22342; AAA32477.1; -; Genomic_DNA. DR PIR; A30195; XYBPT2. DR SMR; P12427; 1-259. DR REBASE; 2685; M.EcoT2Dam. DR InterPro; IPR002294; D12N6_mtfrase. DR InterPro; IPR012326; Dam. DR InterPro; IPR012263; M_m6A_EcoRV. DR InterPro; IPR012327; MethyltransfD12. DR InterPro; IPR002052; N6_Mtase. DR Pfam; PF02086; MethyltransfD12; 1. DR PIRSF; PIRSF000398; M_m6A_EcoRV; 1. DR PRINTS; PR00505; D12N6MTFRASE. DR TIGRFAMs; TIGR00571; dam; 1. DR PROSITE; PS00092; N6_MTASE; 1. KW DNA replication; Methyltransferase; Transferase. FT CHAIN 1 259 DNA adenine methylase. FT /FTId=PRO_0000087989. FT BINDING 7 7 S-adenosyl-L-methionine (By similarity). FT BINDING 11 11 S-adenosyl-L-methionine; via amide FT nitrogen (By similarity). FT BINDING 50 50 S-adenosyl-L-methionine (By similarity). FT BINDING 171 171 S-adenosyl-L-methionine (By similarity). SQ SEQUENCE 259 AA; 30439 MW; B3551303CFE71FD5 CRC64; MLGAIAYTGN KQSLLPELKP HFPKYDRFVD LFCGGLSVSL NVNGPVLAND IQEPIIEMYK RLINVSWDDV LKVIKQYKLS KTSKEEFLKL REDYNKTRDP LLLYVLHFHG FSNMIRINDK GNFTTPFGKR TINKNSEKRF NHFKQNCDKI IFSSLHFKDV KILDGDFVYV DPPYLITVAD YNKFWSEEEE KDLLNLLDSL NDRGIKFGLS NVLEHHGKEN TLLKEWSKKY NVKHLNKKYV FNIYHSKEKN GTDEVYIFN //